Guisheng GAO，Qiumei SHl*，Muxiang GE，Juan CHEN，Yongmei SU，Yuling DONG，Hongsheng HAN，Qingchun SONG

1.Hebei Provincial Key Laboratory of Preventive Veterinary Medicine/Hebei Normal University，Qinhuangdao 066600，China；

2.Qinhuangdao Liangfeng Marine Ecological Technology Development Co.，Ltd.，Qinhuangdao 066600，China；

3.Hebei Haohai Biotechnology Co.，Ltd.，Qinhuangdao 066004，China

Effects of Salinity and Freezing on Freshness of Crucian

Guisheng GAO1，Qiumei SHl1*，Muxiang GE1，Juan CHEN1，Yongmei SU1，Yuling DONG1，Hongsheng HAN2，Qingchun SONG3

1.Hebei Provincial Key Laboratory of Preventive Veterinary Medicine/Hebei Normal University，Qinhuangdao 066600，China；

2.Qinhuangdao Liangfeng Marine Ecological Technology Development Co.，Ltd.，Qinhuangdao 066600，China；

3.Hebei Haohai Biotechnology Co.，Ltd.，Qinhuangdao 066004，China

Salt tolerance and freeze preservation of crucian are always important concerns of people.There are rare reports on effect of different salinity on freshness and quality.However，water salinity will directly affect osmotic pressure in fish，thereby affecting meat quality.In this study，the tolerance of crucian to different salinities （1‰，2‰，5‰，8‰，10‰ and 15‰）was investigated.In addition，the effect of different salinity on freezing freshness and quality of crucian was studied. The results showed that the tolerance threshold of crucian to salinity was about 8‰，and the freezing quality of crucian in water with salinity of 8‰ was significantly better than that in fresh water.

Crucian；Salinity；Salt tolerance；Freshness

M ost freshwater fishes have certain salt tolerance.Water salinity is an important environment factor affecting fish quality，and itchangesbodycomponents through regulating osmotic pressure of fish，leading to changes in fish quality.Salt has certain hardness （2-2.5 grade），so it has certain massaging effect.Moreover，salt will not scratch the skin，and it will make the skin exquisite and smooth instead.Sodium chloride has functions of sterilizing，diminishing inflammation and relieving itching［1］.Freeze preservation can maintain food freshness，nutritional value and original flavor to the greatest extent.Compared with that of refrigerated preservation，the storability of freeze preservation is 1.4 times higher［2］.Foreign scholars have also conducted researches on refrigerated and freeze preservation of fishes［3］. With the steady and rapid development of freshwater fishery， fish preservation becomes more and more important to its quality.However，there are no systematic and in-depth researches on effect of freeze preservation on fish quality.Therefore，it is necessary to conduct scientific research on freeze preservation of freshwater fishes.

In this study，the tolerance of crucian to several salinities，as well as the effect of freeze preservation on crucian quality，was investigated.The growth status of crucian in differentsalinity waters，and its quality variation under the condition of freeze preservation，was explored，providing some reference for low-temperature storage and processing and quality control of freshwater fishes and development of relevant equipment.

Materials and Methods

Materials

The live crucians with average length of 20 cm and average weight of 250 g were purchased from an aquatic product market in Jieshi Mountain，Changli County，Qinhuangdao City，Hebei Province.The used instrumentsand equipment included pH meter，high pressure steam sterilizer，incubator，nutrient agar，etc.

Salt tolerance test

In the aquariums，different-salinity（1‰，2‰，5‰，8‰，10‰ and 15‰）waters were prepared.After the aeration，crucians were placed in the waters.There were two replicates for each salinity treatment.The swimming and performance of crucians were observed closely and recorded.Before the transplanting into salt waters，there were small bleeding spots，caused by little traumas，and little scale loss in the pectoral fins and pelvic fins，and tissue loss in caudal fins of crucians due to various seasons，such as salvage and collision.During the test，the performance of crucians，as well as the recovery conditions and recovery times of traumas，were observed.

Freeze preservation test

According to the results of salt tolerance test，three salinities（5‰，8‰and 10‰）with higher crucian survival rate were selected for the conduction of freeze（-20℃）preservation test.In addition，one freshwatertreatment was designed as the control.The sensory indexes，pH value，moisture content and bacterial colony number of crucian were measured［4］.

Sensory measurementAfter the ice around the crucians melt naturally，the crucians were cleaned with distilled water.The back muscles of crucians were collected.Under the normal conditions，the smell and elasticity of crucian back muscle were observed with eyes［8］.The sensory evaluation standards were shown in Table 1.

Table 1 Sensory evaluation standards

pH measurement A certain amount（2.0 g）of back muscle of each crucian was ground，and then dissolved in a certain volume（20 ml）of cooled boiling water.The mixtures were stood for 30 min and filtered.The collected filtrates were directly used for pH measurement［6］.

Table 2 Mortality rate of crucians under different salinity

Results and Analysis

Results of salt tolerance test

When crucians were first placed into the waters with salinities of 10‰and 15‰，they were uneasy，swam back and forth，floated their heads，and even jumped.However，in the other waters，the crucians appeared normal.After 12 h，the bleeding spots in dorsal fins and anal fins of crucians in the low-salinity （1‰，2‰and 5‰）waters began to fester.After 24 h，the waters at various salinities all turned clear.The crucians in various aquariums swam normally，and their breathing and other functions were all normal by visual inspection.The crucian body injury in waters with salinities of 8‰and 10‰was lighter，and the bleeder area was also smaller.After 48 h，the crucians in the waters with salinities of 2‰and 5‰swam slowly，and their response to touching also became slow. Even worse，the bleeders in their heads，pectoral fins and pelvic fins were aggravated.In the water with salinity of 8‰，the lips swelling of crucians was reduced，and their bleeding spots were also reduced.In the water with salinity of 10‰ ，the crucians swam slowly，and their eyes were dimmed.After 72 h，in the water with salinity of 2‰，the crucian skin became pale and ulcerated，and more bleeding spots were shown in the mouths and tail fins of crucians.In the water with salinity of 8‰，the injury in crucian head was relatively light，but high-density bleeding spots appeared below crucian operculum.

Results of freeze preservation test Sensory measurement After the crucians were frozen，their bodies became harder，and the quality inspection also became more difficult.Therefore，during the inspection，only crucian eyeballs，skin color and hardness were inspected.Otherwise，the crucian body could be cut apart，thus the internal quality could be inspected. The sensory evaluation results of freeze-preserved crucians under different salinities were shown in Table 3. As shown in Table 3，the crucian’s eyes were intact in all the waters.With the increased salinity，crucian’s eyes became bright and glossy.In freshwater，crucian gill filaments had more mucus，and with the increased salinity，the mucus was still normal and bright red.In the freshwater，crucian muscle was relaxed to some extent.But with the increased salinity，crucian muscle became tight and elastic.There was little mucus on crucian body surface.Inthe freshwater，crucian tail fin was intact，and easy to be broken；the anus was not jutting.With the increased salinity，crucian anus contracted，but was not swelling or jutting.

Table 3 Sensory evaluation of freeze-preserved crucian under different salinity

Table 4 pH value of freeze-preserved crucian muscle under different salinity

Table 6 Total bacterial colony number in freeze-preserved crucian muscle under different salinity

pH measurement Raw meat quality will directly determine the quality of meat products，and pH value is one of the important indicators.If pH value is relatively low，meat will has longer shelf life and better flavor.As shown in Table 4，crucian muscle pH value changed greatly after the melt.The post-melt muscle pH value of crucian in the water with salinity of 10‰ was closest to the initially measured value（6.88），while the post-melt muscle pH value of crucian in the freshwater was lower than that of fresh crucian.

Moisture content determination

Moisture content will not only affect color，aroma，taste，nutritional components，juiciness，tenderness and edible quality，but also directly affect economic value of meat.As shown in Table 5，when water salinity was higher than 8‰ ，moisture contentin freeze-preserved crucian muscle was reduced，but the dry matter content in crucian muscle was trended to be increased with the increase of water salinity.

Measurement of bacterial colony number in frozen fish As shown in Table 6，before the freezing，the total bacterial colony numbers（diluted 100 times）in crucian muscle under different salinities were 40×104（freshwater），32×104（salinity of 5‰），27×104（salinity of 8‰）and 25×104（salinity of 10‰），respectively.After the freeze preservation，the total bacterial colony numbers in different-salinity waters were significantly reduced，and the total bacterial colony number in post-melt crucian muscle was reduced gradually with the increase of water salinity.

Conclusions

Crucian flesh has higher moisture content，so it is easy to be rotted.If the rotted crucian flesh is not handled in a timely manner，the proteins and amino acids inside the flesh will be decomposed under the action of microorganisms and a series of enzymes，seriously affecting the quality of crucian flesh.In order to ensure the quality of crucian flesh，refrigerated preservation or freeze preservation is an important way.Crucian has certain salt tolerance.The salinity in freshwater is generally lower than 0.5‰.In general，freshwater fish can live a normal life in the water with salinity lower than 5‰. The excessively high salinity in water will produce certain effects on growth and reproduction of freshwater fish.

Salt tolerance of crucian

Zang et al.［7］reported that different-specification fish ofthe same species has different-degree salt tolerance.There are chemical receptors in the mouth of fish.Thus，when water salinity is changed，the fish will feel it. Once water salinity is changed，the phenomenon of swallowing water will appear in fish.Ion concentration in fish body fluid directly affects environmental homeostasis in fish body.To ensure the balance of osmotic pressure，fish needs to consume the energy of the body.Therefore，salinity change，as a result of energy change，will also directly affect the growth of fish.From the results above，it can be concluded that freshwater crucian can adapt to a certain range of salinity，but not too high salinity.The water salinity cannot exceed 5‰.Otherwise，the growth status of crucian will be greatly aggra-vated，and death of crucian will even occur.However，the water with appropriate salinity will produce certain positive influence on physiological functions and meat quality of crucian.For example，the sensory quality of freezepreserved crucian in the water with salinity of 8‰ is better than those in the other waters.In freshwater，would in crucian is easy to be infected by pathogenic microorganisms.However，in the water with salinity of 8‰，the infection rate of crucian is smaller.However，the survival rate of crucian in high-salinity water is lower than that in freshwater.Therefore，crucian cannot be farmed in high-salinity water for a long time.Within the tolerance range，crucian can only dunked in high-salinity water for a short time for disinfection.The high-salinity water bath cannot be used as a recipe.Li et al.［8］found that the salt tolerance of fish can be improved after appropriate domestication.Although the crucians were not farmed in high-salinity water for a long time in this study，it could be expected that the salt tolerance of crucians would be improved.

Freezing effect under different salinity

The biochemical changes after the death have important influence on decreased freshness and quality change of freshwater fish.Although freeze preservation cannot stop biochemical changes，it can slow the changes.After the death of crucian，the glycogen in its organizations will be converted into lactic acid through anaerobic decomposition.At the same time，phosphocreatine is decomposed into inorganic phosphate and creatine. The exothermic reactions above produce a lot of heat，increasing body temperature of fish.If the fish is not refrigerated timely，the biochemical reactions will be sped up，affecting freshness of fish flesh.After the test in this study，crucian freshness differed greatly among different salinities of waters.Live crucian had blight color and firm and elastic body，and its cut surface was shiny.The freshness of freeze-preserved crucians in the salt waters was significantly better than that in the freshwater.In addition，in the water with salinity of 8‰，crucians had bright and lustrous eyeballs，firm scales and elastic and solid muscle，and their sensory quality was significantly higher than that of crucians in the other waters.In conclusion，crucians should be better preserved in water with salinity of 8‰ before their freeze preservation.

Effects of freeze preservation on pH value and moisture content of crucian

Previous studies have shown that pH value of samples is reduced first and then decreased under the conditions of refrigeration and freezing.In the early stage，liver glycogen is degraded into creatin under anaerobic condition，leading to decreased pH value of muscle.At the same time，the energy released by ATP decomposition in muscle increases body temperature，leading to protein acidic coagulation and muscle rigidity.However，muscle turns soft gradually due to ATP decomposition.Proteins are decomposed into a series of intermediate products，amino acids and soluble nitrogen-containing substances，as well as alkaline substances，increasing muscle pH value［9］.Raw meat quality directly determines the quality of meat products，and the pH is one of the important indicators.If pH value is relatively low，meat will has longer shelf life and better flavor［10］.If pH value is relatively high，meat will has better color.The quality of normal meat is within the two standards above.If the measured pH value shows great difference with the normal level，the meat quality is generally considered to be poor.However，if the measured pH value is close to the normal level，the meat is considered to be qualified and to have good hygienic quality.In this study，the crucian muscle pH value changed after the melt，and the postmelt muscle pH value of crucian in the water with salinity of 10‰was closest to the initially measured pH value（6.88）.It suggests that certain salinity has more positive influence on freshness of freeze-preserved crucian. Moisture content in muscle not only affects the flesh color，aroma，taste，nutrition，juiciness，tenderness and edible quality，but also directly affects the economic value.When the water salinity was higher than 8‰，the moisture content in freeze-preserved crucian muscle was reduced with the increase of salinity，but the dry matter content in crucian was trended to be increased. This phenomenon shows that when the fish is placed in hypertonic water，it will be dehydrated，and under the severe condition，the fish will die.The total bacterial colony number in postmeltcrucianmusclewasreduced gradually with the increase of salinity，which was because that sodium chloride has inhibitory effect on several common bacteria.

Discussion

Crucian can adapt the salinity lower than 8‰.The quality of freeze-preserved crucian in the water with salinity of 8‰ is significantly higher than that in freshwater.

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Responsible editor：Tingting XU

Responsible proofreader：Xiaoyan WU

鹽度及冰凍對(duì)鯽魚(yú)保鮮效果的影響

高桂生1，史秋梅1*，葛慕湘1，陳娟1，蘇詠梅1，董玉玲1，韓紅升2，宋青春3（1.河北科技師范學(xué)院/河北省預(yù)防獸醫(yī)學(xué)重點(diǎn)實(shí)驗(yàn)室，河北秦皇島066600；2.秦皇島糧豐海洋生態(tài)科技股份有限公司，河北秦皇島066600；3.河北皓海生物科技有限公司，河北秦皇島066004）

淡水鯽魚(yú)的耐鹽性及冰凍保鮮一直是人們關(guān)注的一個(gè)重要問(wèn)題。而不同鹽度對(duì)保鮮品質(zhì)的影響很少報(bào)道。但水體鹽度直接影響?hù)~(yú)體滲透壓進(jìn)而影響肉質(zhì)品質(zhì)。該試驗(yàn)研究了鯽魚(yú)對(duì)鹽度1‰、2‰、5‰、8‰、10‰、15‰的耐鹽性，以及不同鹽度的冷凍保鮮效果。研究結(jié)果表明，鯽魚(yú)的耐鹽性為8‰以下，在8‰的水體中進(jìn)行冷凍保存效果明顯高于淡水保存的鯽魚(yú)。

鯽魚(yú)；鹽度；耐鹽性；保鮮

河北省科技廳獎(jiǎng)勵(lì)性后補(bǔ)助項(xiàng)目（15926620H）；秦皇島市科技局科技支撐計(jì)劃（201401A067，201402B043）。

高桂生（1964-），男，河北玉田人，副教授，碩士，從事水產(chǎn)養(yǎng)殖動(dòng)物疾病防治方面的研究，E-mail：Ggs1964@163.com。*通訊作者，教授，博士，從事動(dòng)物傳染病防治方面的研究，E-mail：shiqiumei@126.com。

2015-09-08

Supported by Incentive Program Subsidized by Department of Science and Technology of Hebei Province（15926620H）；Key Technology Research and Development Program of Science and Technology Bureau of Qinhuangdao City（201401A067，201402B043）.

*Corresponding author.E-mail:shiqiumei@126.com

Received:September 8，2015 Accepted:November 21，2015

修回日期 2015-11-21