張軼　王寅　吳劍　李龍江

[摘要] 目的 通過口腔鱗癌細胞中過表達microRNA-200b，檢測口腔鱗癌細胞E-鈣黏著蛋白表達是否有所上調;并檢測E-鈣黏著蛋白表達上調對口腔鱗癌細胞生物學特性的影響。 方法 通過目的基因過表達慢病毒載體構建（has-miR 200b-LV），轉染舌癌細胞株Tca8113，檢測轉染后舌癌細胞株Tca8113的E-鈣黏著蛋白的表達及轉染后舌癌細胞株Tca8113的侵襲和轉移情況。 結果 實驗組通過Real-time PCR數值分析顯示miR-200b基因表達定量分析平均值比較，組間差異有統(tǒng)計學意義（P<0.05）;轉染后的Tca8113細胞，通過Real-time PCR數值分析顯示實驗組中E-cadherin基因表達定量分析平均值比較，組間差異有統(tǒng)計學意義（P<0.05）;Transwell侵襲實驗中，空白組和對照組穿膜細胞數分別為（29.3±4.5）和（20.2±3.3），與實驗組（4.3±2.1）比較，差異有統(tǒng)計學意義（P<0.05）;生長曲線顯示microRNA-200b過表達病毒感染的腫瘤細胞生長速度明顯減慢。 結論 microRNA-200b的表達可以調控E-鈣黏著蛋白的表達，E-鈣黏著蛋白的高表達可以抑制腫瘤的侵襲和轉移。

[關鍵詞] microRNA-200b;E-鈣黏著蛋白;侵襲;轉移

[中圖分類號] R739.85 ? ? ? ? ?[文獻標識碼] A ? ? ? ? ?[文章編號] 1674-0742（2020）10（a）-0033-03

microRNA-200b Regulating E-cadherin in Oral Squamous Cell Carcinoma

ZHANG Yi1，2， WANG Yin2， WU Jian2， LI Long-jiang2

1.Department of Stomatology， Zhongshan Hospital， Xiamen University， Xiamen， Fujian Province， 361004 China; 2.Surgery of Head and Neck Oncology， West China Hospital of Stomatology， Sichuan University， Chengdu， Sichuan Province， 610041 China

[Abstract] Objective To detect whether the expression of E-cadherin in oral squamous cell carcinoma cells is up-regulated by overexpressing microRNA-200b in oral squamous cell carcinoma cells; and to detect the effect of up-regulation of E-cadherin expression on the biological characteristics of oral squamous cell carcinoma cells influences. Methods The target gene overexpression lentiviral vector （has-miR 200b-LV） was constructed and transfected into the tongue cancer cell line Tca8113. The expression of E-cadherin in the tongue cancer cell line Tca8113 after transfection and the tongue cancer cell line after transfection were detected. Results The real-time PCR numerical analysis of the experimental group showed that the average value of miR-200b gene expression quantitative was compared， and the difference between the groups was statistically significant （P<0.05）; the transfected Tca8113 cells passed Real-time Time PCR numerical analysis showed that the average value of E-cadherin gene expression quantitative analysis in the experimental group was （compared）， and the difference between the groups was statistically significant （P<0.05）; in the Transwell invasion experiment， the blank group and the control group had permeabilized cells numbers were （29.3±4.5） and （20.2±3.3）， which were statistically different from the experimental group （4.3±2.1） （P<0.05）; the growth curve showed the growth rate of microRNA-200b overexpression virus-infected tumor cells was significantly slowed down. Conclusion The expression of microRNA-200b can regulate the expression of E-cadherin， and the high expression of E-cadherin can inhibit tumor invasion and metastasis.

綜上所述，microRNA-200b的表達可以明顯上調E-鈣黏著蛋白的表達， E-鈣黏著蛋白的高表達可以抑制腫瘤的侵襲和轉移。

[參考文獻]

[1] ?李加麗.癌癥患者生命質量的影響因素及護理干預的研究進展[J].醫(yī)療裝備， 2017，30（14）：187-188.

[2] ?王清富. 溶血磷脂酸通過調控PI3K/AKT信號通路對骨肉瘤細胞黏附和遷移的作用[J]. 腫瘤學雜志，2019，25（4）：320-324.

[3] ?Gan W J ， Wang J R ， Xiao-Li Zhu.et al. RARγ-induced E-cadherin downregulation promotes hepatocellular carcin oma invasion and metastasis[J].Journal of Experimental & Clinical Cancer Research， 2016， 35（1）：164.

[4] ?Kurata A， Yamada M， Ohno SI，et al. Expression level of microRNA-200c is associated with cell morphology in vitro and histological differentiation through regulation of ZEB1/2 and E-cadherin in gastric carcinoma[J].Oncology Reports， 2018.39（1）： 91-100.

[5] ?陸旭， 周闖， 李仁鋒， 等. Kindlin-2誘導膽囊癌細胞上皮間質轉化促進其侵襲轉移的實驗研究[J].中華外科雜志，2018，56（8）：617-622.

[6] ?劉莉，楊永姣，陳業(yè)剛，等.細胞粘附分子-1抑制膀胱癌細胞侵襲轉移的機制研究[J].重慶醫(yī)科大學學報，2018，43（4）：40-43.

[7] ?Yue Hu，Yayuan Zheng，Mingrui Dai，et al. G9a and HDAC s are crucial for Snail2﹎ediated Eadherin repression and metastasis in hepatocellular carcinoma[J].Cancer Science， 2019，110（11）： 3442-3452.

[8] ?Li C， Liu J， Zhang Q， et al. Upregulation of E-cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder cancer cells by inhibiting β-catenin/TCF target genes[J].International Journal of Oncol ogy，2018，52（6）：1815-1826.

（收稿日期：2020-07-05）