董海波 倪迎冬

摘 要：選擇8頭安裝瘤胃瘺管的泌乳中期山羊隨機分為2組分別飼喂高精料日糧（60%干物質(zhì)，HC組）和低精料日糧（40%干物質(zhì)，LC組），實驗周期9周，期間采集瘤胃液，血液及肝臟組織。研究長期飼喂高精料日糧后肝臟中代謝底物的變化規(guī)律。實驗結(jié)果顯示：飼喂9周后，高精料日糧導(dǎo)致瘤胃液pH顯著下降；乳脂，乳蛋白也顯著下降；血漿中瘦素leptin（P=0.05），非酯化脂肪酸NEFA（P=0.07），葡萄糖glucose（P=0.014）均顯著上升；血漿皮質(zhì)醇cortisol（P<0.05）水平顯著上升。肝臟中糖皮質(zhì)激素受體GR蛋白與基因表達一致均顯著顯著下調(diào)（P<0.05）；IL-1β，SAA，CRP及HP基因表達均顯著上升（P<0.05）。高通量芯片分析顯示與炎癥反應(yīng)，脂代謝，蛋白代謝以及糖代謝的關(guān)鍵基因均發(fā)生顯著上調(diào)或下調(diào)，代謝調(diào)控網(wǎng)絡(luò)發(fā)生變化。結(jié)論 急性期反應(yīng)與炎癥反應(yīng)可能促進肝臟中能量重分配并最終導(dǎo)致乳品質(zhì)下降。

關(guān)鍵詞：乳品質(zhì) 急性期反應(yīng) 炎癥反應(yīng) 代謝 基因表達 肝臟 泌乳山羊

Long-term Effects of SARA on Milk Quality and Hepatic Gene Expression in Lactating Goats Fed a High-concentrate Diet

Dong Haibo Ni Yingdong

（Nanjing Agricultural University）

Abstract： Eight mid-lactating goats with rumen cannulas were randomly assigned to two groups. For 9 weeks， the treatment group was fed a high-concentrate diet （60% concentrate of dry matter， HC） and the control group was fed a low-concentrate diet （40% concentrate of dry matter， LC）. Ruminal fluid， plasma， and liver tissues were sampled， microarray techniques and real-time polymerase chain reaction were used to evaluate metabolic parameters and gene expression in liver. Feeding a 60%-concentrate diet for 9 weeks resulted in a significant decrease in rumen pH. Changes in fat and protein content also occurred， which negatively affected milk quality. Plasma levels of leptin （P=0.058）， non-esterified fatty acid （P=0.071）， and glucose （P=0.014） increased markedly in HC group. Plasma cortisol concentration was significantly elevated in the treatment group （P<0.05）. Expression of the glucocorticoid receptor protein gene was significantly down-regulated （P<0.05） in the liver. The expression of genes for interleukin 1b， serum amyloid A， C-reactive protein， and haptoglobin mRNA was significantly increased （P<0.05） in the HC group. GeneRelNet analysis showed that gene expression involved in inflammatory responses and the metabolism of lipids， protein， and carbohydrate were significantly altered by feeding a high-concentrate diet for 9 weeks.

Key Words： Milk quality； Acute phase response； Inflammatory response； Metabolism； Gene expression； Liver； lactating goats

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