梁 歡， 尚慶茂

(農(nóng)業(yè)部園藝作物生物學(xué)與種質(zhì)創(chuàng)制重點實驗室，中國農(nóng)業(yè)科學(xué)院蔬菜花卉研究所，北京 100081)

梁 歡， 尚慶茂*

(農(nóng)業(yè)部園藝作物生物學(xué)與種質(zhì)創(chuàng)制重點實驗室，中國農(nóng)業(yè)科學(xué)院蔬菜花卉研究所，北京 100081)

多孔一體式穴盤育苗具有節(jié)能、省力、適合機械化操作、生產(chǎn)效率高等顯著特點，是我國蔬菜集約化育苗的主要形式。與傳統(tǒng)營養(yǎng)缽育苗相比，穴盤育苗采用的人工混配輕型基質(zhì)，通氣孔隙度增大，單株基質(zhì)容量減小，管理不當(dāng)極易導(dǎo)致幼苗礦質(zhì)養(yǎng)分盈虧，實時準(zhǔn)確判斷蔬菜穴盤苗養(yǎng)分狀況對壯苗育成至關(guān)重要。目前，蔬菜礦質(zhì)營養(yǎng)診斷方法主要分為培養(yǎng)介質(zhì)(包括土壤和人工混配基質(zhì))分析法和組織分析法[1-2]，介質(zhì)分析僅能反映根系可從介質(zhì)獲得的潛在營養(yǎng)水平，分析結(jié)果常因取樣方法、貯存條件和養(yǎng)分提取技術(shù)而產(chǎn)生偏差。組織分析剔除了介質(zhì)特性、環(huán)境因素對礦質(zhì)養(yǎng)分吸收的影響，更能直接反映植株礦質(zhì)營養(yǎng)狀況，研究與應(yīng)用也更加廣泛。

1 材料與方法

1.1 試驗材料

共采集了番茄17個品種，‘中雜9號’(Zhongza 9)、‘中雜15號’(Zhongza 15)、‘中雜101號’(Zhongza 101)、‘中雜105號’(Zhongza 105)、‘中雜106號’(Zhongza 106)、‘中雜108號’(Zhongza 108)、‘中雜109號’(Zhongza 109)、‘美櫻2號’(Meiying 2)、‘北京櫻桃番茄’(Beijing cherry tomato)來自中國農(nóng)業(yè)科學(xué)院蔬菜花卉研究所。‘佳紅4號’(Jiahong 9)、‘佳紅5號’(Jiahong 5)、‘佳粉18號’(Jiafen 18)、‘硬粉8號’(Yingfen 8)、‘仙客1號’(Xianke 1)、‘仙客5號’(Xianke 5)、‘京丹紅小果’(Jingdan red cherry tomato)、‘京丹黃小果’(Jingdan yellow cherry tomato)來自北京京研益農(nóng)科技發(fā)展中心。

化學(xué)測定所用試劑均購自國藥集團化學(xué)試劑北京有限公司，AR級。

1.2 試驗設(shè)計

1.3 測定方法

每重復(fù)隨機選取番茄穴盤苗25株，將下胚軸、莖(子葉節(jié)至頂端生長點)、各葉位葉柄和葉片分別取樣，剪成1 cm小段(下胚軸、莖、葉柄)或1 cm×1 cm小塊(葉片)，壓蒜器榨取汁液，收集至1.5 mL離心管，4℃冰箱保存?zhèn)錅y。

采用Microsoft Excel 2003和SAS 9.2進行統(tǒng)計分析。

2 結(jié)果與分析

3 討論

4 結(jié)論

表4 番茄穴盤苗不同組織-N閾值

[1] Prado R D M, Caione G. Plant analysis[J]. Soil Fertility, 2012, 115-134.

[2] Hartz T K, Smith R F, LeStrange Metal. On-farm monitoring of soil and crop nitrogen status by nitrate-selective electrode[J]. Communications in Soil Science and Plant Analysis, 1993, 24(19-20): 2607-2615.

[3] Peng S, García F V, Laza R Cetal. Adjustment for specific leaf weight improves chlorophyll meter's estimate of rice leaf nitrogen concentration[J]. Agronomy Journal, 1993, 85(5): 987-990.

[4] Gianquinto G, Sambo P, Borsato D. Determination of SPAD threshold values for the optimisation of nitrogen supply in processing tomato[J]. Acta Horticulturae, 2004, 700： 159-166.

[5] Sandoval-Villa M, Wood C W, Guertal E A. Tomato leaf chlorophyll meter readings as affected by variety, nitrogen form, and nighttime nutrient solution strength[J]. Journal of Plant Nutrition, 2002, 25(10): 2129-2142.

[6] Fontes P C R, Araujo C. Use of a chlorophyll meter and plant visual aspect for nitrogen management in tomato fertigation[J]. Journal of Applied Horticulture, 2006, 8(1): 8-11.

[7] 張延麗, 田吉林, 翟丙年, 諸海濤. 不同施氮水平下黃瓜葉片 SPAD 值與硝態(tài)氮含量及硝酸還原酶活性的關(guān)系[J]. 西北農(nóng)林科技大學(xué)學(xué)報(自然科學(xué)版), 2009, 37(1): 189-193. Zhang Y L, Tian J L, Zhai B N, Zhu H T. Relationship between leaf SPAD values and the nitrate content and nitrate reductase activity in cucumber at different nitrogen rates[J]. Journal of Northwest A&F University(Natural Science Edition), 2009, 37(1): 189-193.

[8] 范燕萍, 余讓才, 陳建勛,楊瑞陶. 氮素營養(yǎng)脅迫對匙葉天南星生長及光合特性的影響[J]. 園藝學(xué)報, 2000, 27(4): 297-299. Fan Y P, Yu R C, Chen J X, Yang R T. Effects of nitrogen nutrition stress on the growth and photosynthetic characteristics of spathiphyllum palls hort[J]. Acta Horticulturae Sinica, 2000, 27(4): 297-299.

[9] 毛罕平, 徐貴力, 李萍萍. 番茄缺素葉片的圖像特征提取和優(yōu)化選擇研究[J]. 農(nóng)業(yè)工程學(xué)報, 2003, 19(2): 133-136. Mao H P, Xu G L, Li P P. Extracting and selecting features of leaf images for diagnosis nutrient deficiency diseases in tomatoes[J]. Transactions of the Chinese Society of Agricultural Engineering, 2003, 19(2)：133-136.

[10] 柴阿麗, 李寶聚, 王倩, 等. 基于計算機視覺技術(shù)的番茄葉片葉綠素含量的檢測[J]．園藝學(xué)報, 2009, 36(1): 45-52. Chai A L, Li B J, Wang Qetal. Detecting chlorophyll content of tomato leaves with technology of computer vision[J]. Acta Horticulturae Sinica, 2009, 36(1): 45-52.

[11] Leigh R A, Johnston A E. Nitrogen concentrations in field-grown spring barley: an examination of the usefulness of expressing concentrations on the basis of tissue water[J]. Journal of Agricultural Science, 1985, 105(2): 397-406.

[12] Huett D, Rose G. Diagnostic nitrogen concentrations for tomatoes grown in sand culture[J]. Australian Journal of Experimental Agriculture, 1988, 28(3): 401-409.

[13] Hochmuth G J. Efficiency ranges for nitrate-nitrogen and potassium for vegetable petiole sap quick tests[J]. Horttechnology, 1994, 4(3): 218-222.

[14] 張衛(wèi)建, 黃丕生, 陸士龍. 單季中稻體內(nèi)氮營養(yǎng)水平診斷研究[J]. 江蘇農(nóng)業(yè)學(xué)報, 1996, 12(3): 15-19. Zhang W J, Huang P S, Lu S L. Studies on rapid determination of plant nitrogen status in single crop midseason rice[J]. Jiangsu Journal of Agricultural Science, 1996, 12(3): 15-19.

[15] 李文才, 林振武, 湯玉瑋. 硝酸還原酶的研究-Ⅴ. 棉花硝酸還原酶活力與NO3-N含量的關(guān)系[J]. 作物學(xué)報, 1983, 9(2): 93-97. Li W C, Lin Z W, Tang Y W. Studies on nitrate reductase Ⅴ.Relation of nitrate reductase activity to nitrate content in cotton[J]. Acta Agronomica Sinica, 1983, 9(2): 93-97.

[16] Olsen J K, Lyons D J. Petiole sap nitrate is better than total nitrogen in dried leaf for indicating nitrogen status and yield responsiveness of capsicum in subtropical Australia[J]. Australian Journal of Experimental Agriculture, 1994, 34(6): 835-843.

[17] 周阮寶, 谷麗萍, 茆敦俊, 周嘉槐. 介紹兩種簡易測定硝態(tài)氮的比色方法[J]. 植物生理學(xué)通訊, 1991, 27(4): 299-301. Zhou R B, Gu L P, Mao D J, Zhou J H. Two simple colorimetric methods for NO3-N[J]. Plant Physiology Communications, 1991, 27(4): 299-301.

[18] 王西娜, 王朝輝, 陳寶明, 李生秀. 不同品種菠菜葉柄和葉片的硝態(tài)氮含量及其與植株生長的關(guān)系[J]. 植物營養(yǎng)與肥料學(xué)報, 2005, 11(5): 675-681. Wang X N, Wang C H, Chen B M, Li S X. Nitrate accumulation in petiole and blade of different spinach cultivars and its relation to plant growth[J]. Plant Nutrition and Fertilizer Science, 2005, 11(5): 675-681.

[19] 姚建武, 艾紹英, 柯玉詩, 等. 幾個菜心品種硝酸鹽累積差異的研究[J]. 土壤與環(huán)境, 2002, 11(3): 255-257. Yao J W, Ai S Y, Ke Y Setal. Differences of nitrate accumulation among some Chinese flowering cabbage varieties[J]. Soil and Environmental Sciences, 2002, 11(3): 255-257.

[20] 劉麗, 甘志軍, 王憲澤. 植物氮代謝硝酸還原酶水平調(diào)控機制的研究進展[J]. 西北植物學(xué)報, 2004, 24(7): 1355-1361. Liu L, Gan Z J, Wang X Z. Advances of studies on the regulation of nitrate metabolism of plants at nitrate reductase level[J]. Acta Botanica Boreali-Occidentalia Sinica, 2004, 24(7): 1355-1361.

[21] Farneselli M, Tei F, Simonne E. Reliability of petiole sap test for N nutritional status assessing in processing tomato[J]. Journal of Plant Nutrition, 2014, 37(2): 270-278.

[23] 柳勇, 何江華, 杜應(yīng)瓊, 等. 施氮, 鉀和鉬對菠菜不同生長階段硝態(tài)氮積累影響研究初報[J]. 植物營養(yǎng)與肥料學(xué)報, 2005, 11(3): 363-368. Liu Y, He J H, Du Y Qetal. Effects of N, K and Mo application on the nitrate accumulation at different growth stages of spinach[J]. Plant Nutrition and Fertilizer Science, 2005, 11(3): 363-368.

[24] 周根娣, 盧善玲. 磷鉀肥、 光照、 貯藏加工對蔬菜硝酸鹽含量的影響[J]. 上海農(nóng)業(yè)學(xué)報, 1991, 7(2): 53-56. Zhou G D, Lu S L. Effects of phosphorus and potassium fertilizers, shining, storage and processing on nitrate content in vegetable[J]. Acta Agriculturae Shanghai, 1991, 7(2): 53-56.

[25] 楊暹, 關(guān)佩聰. 氮鉀營養(yǎng)與花椰菜氮素代謝和產(chǎn)量的初步研究[J]. 華南農(nóng)業(yè)大學(xué)學(xué)報, 1994, 15(1): 85-90. Yang X, Guan P C. The preliminaru study on the relationship between N K nutrition and nitrogen metabolism, curd yied in cauliflower(Brassicaoleraceavar.botrytisL.)[J]. Journal of South China Agriculture University, 1994, 15(1): 85-90.

[26] 齊炳林, 曹翠玲, 王菲, 等. 磷脅迫對豇豆幼苗硝酸還原酶活性和硝態(tài)氮含量的影響[J]. 干旱地區(qū)農(nóng)業(yè)研究, 2010, 28(1): 147-151. Qi B L, Cao C L, Wang Fetal. Influence of low phosphorus on nitrate reductive activity and NO3-N content in cowpa seedling[J]. Agriculture Research in the Arid Areas, 2010, 28(1): 147-151.

[27] 馬光恕, 廉華, 王彥宏, 等. 磷素不同用量對萵苣硝酸鹽積累的影響[J]. 中國土壤與肥料, 2009,(4): 68-70. Ma G S, Lian H, Wang Y Hetal. Effects of different amount phosphorus elements on the nitrate accumulation of lettuce[J]. Soil and Fertilizer Sciences in China, 2009,(4): 68-70.

[28] Proietti S, Moscatello S, Leccese Aetal. The effect of growing spinach(SpinaciaoleraceaL.) at two light intensities on the amounts of oxalate, ascorbate and nitrate in their leaves[J]. Journal of Horticultural Science and Biotechnology, 2004, 79(4): 606-609.

[29] Liu W K, Yang Q C. Effects of short-term treatment with various light intensities and hydroponic solutions on nitrate concentration of lettuce[J]. Acta Agriculturae Scandinavica(Section B-Soil and Plant Science), 2012, 62(2): 109-113.

[30] 許振柱, 周廣勝. 植物氮代謝及其環(huán)境調(diào)節(jié)研究進展[J]. 應(yīng)用生態(tài)學(xué)報, 2004, 15(3): 511-516. Xu Z Z, Zhou G S. Research advance in nitrogen metabolism of plant and its environmental regulation[J]. Chinese Journal of Applied Ecology, 2004, 15(3): 511-516.

[32] Zhao C, Liang J, He Jetal. Effects of elevated temperature and nitrogen fertilization on nitrogen metabolism and nutrient status of two coniferous species[J]. Soil Science and Plant Nutrition, 2012, 58(6): 772-782.

LIANG Huan, SHANG Qing-mao*

(KeyLaboratoryofBiologyandGeneticImprovementofHorticulturalCrops,MinistryofAgriculture;InstituteofVegetablesandFlowers,ChineseAcademyofAgriculturalSciences,Beijing100081,China)

2014-02-17 接受日期： 2014-10-16 網(wǎng)絡(luò)出版日期: 2015-02-04

國家自然科學(xué)基金項目(31172001)； 現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項資金(CARS-25)； 公益性行業(yè)科研專項(201303014)； 中國農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程(CAAS-ASTIP-IVFCAAS)資助。

梁歡(1990—)，女，山西臨汾人，碩士研究生，主要從事蔬菜苗期發(fā)育調(diào)控方面的研究。E-mail：lianghuanconf@126.com * 通信作者 E-mail：shangqingmao@caas.cn

S625.5+4； S641.2

A

1008-505X(2015)03-0709-10