亚洲免费av电影一区二区三区,日韩爱爱视频,51精品视频一区二区三区,91视频爱爱,日韩欧美在线播放视频,中文字幕少妇AV,亚洲电影中文字幕,久久久久亚洲av成人网址,久久综合视频网站,国产在线不卡免费播放

        ?

        不同冷凍貯藏時(shí)間對(duì)三種魚(yú)類(lèi)肌肉中孔雀石綠殘留量的影響

        2014-10-27 20:54:55高麗娜等
        河北漁業(yè) 2014年10期
        關(guān)鍵詞:肌肉殘留

        高麗娜等

        摘 要:研究鯉魚(yú)、鰻鱺、鱖魚(yú)在加入不同濃度的孔雀石綠標(biāo)準(zhǔn)樣品且在不同冷凍貯藏時(shí)間下對(duì)孔雀石綠殘留量的影響。三種基質(zhì)中,鰻鱺組織中添加有無(wú)色孔雀石綠降解速率最慢、鱖魚(yú)次之、鯉魚(yú)較快。三種基質(zhì)中添加10 μg/kg、50 μg/kg、100 μg/kg有色孔雀石綠平均降解率分別為57.9%、48.0%、23.0%;無(wú)色孔雀石綠平均降解率分別為46.6%、43.4%、19.8%,有色孔雀石綠較無(wú)色孔雀石綠降解快,低濃度較高濃度降解快。在三種基質(zhì)鯉魚(yú)、鰻鱺、鱖魚(yú)組織中有色和無(wú)色孔雀石綠降解反應(yīng)均符合準(zhǔn)一級(jí)反應(yīng)動(dòng)力學(xué)。分析可能由于鰻鱺組織脂肪較多,其結(jié)合方式影響了有色無(wú)色孔雀石綠的降解。

        關(guān)鍵詞:貯藏時(shí)間;肌肉;孔雀石綠;殘留

        食品安全是重要的民生問(wèn)題,直接關(guān)系到社會(huì)的和諧穩(wěn)定。近年來(lái),我國(guó)水產(chǎn)品質(zhì)量安全事件時(shí)有發(fā)生,對(duì)漁業(yè)的健康發(fā)展造成嚴(yán)重的影響。因藥物殘留超標(biāo)而被退貨、銷(xiāo)毀甚至中斷貿(mào)易往來(lái)事件屢見(jiàn)不鮮。2001年,歐盟檢出中國(guó)對(duì)蝦、蝦仁含有氯霉素,隨后對(duì)其實(shí)施了嚴(yán)格檢查,并提出了蝦類(lèi)氯霉素污染新規(guī)定;2003年7月15日,日本靜岡衛(wèi)生部門(mén)從產(chǎn)自中國(guó)的冷凍鰻魚(yú)中檢出恩諾沙星,對(duì)已售出的8 210 kg冷凍鰻魚(yú)發(fā)出警示并要求立即回收。2005年,日本從中國(guó)進(jìn)口的鰻魚(yú)制品中檢測(cè)出孔雀石綠,對(duì)中國(guó)進(jìn)口烤鰻制品發(fā)出禁止通令??梢?jiàn)藥物殘留問(wèn)題儼然己成為我國(guó)擴(kuò)大水產(chǎn)品國(guó)際貿(mào)易的主要障礙。水產(chǎn)品的質(zhì)量問(wèn)題,已成為制約我國(guó)水產(chǎn)養(yǎng)殖業(yè)進(jìn)一步發(fā)展的瓶頸[1]。

        我國(guó)從2002年開(kāi)始對(duì)水產(chǎn)品藥物殘留實(shí)施監(jiān)控計(jì)劃,農(nóng)業(yè)部每年都有大量經(jīng)費(fèi)用于監(jiān)控孔雀石綠的使用情況,許多國(guó)家級(jí)、部級(jí)、省(市)級(jí)實(shí)驗(yàn)室承擔(dān)其監(jiān)控及檢測(cè)任務(wù)。其結(jié)果的準(zhǔn)確性和科學(xué)性不僅關(guān)系著生產(chǎn)者的切身利益,更考驗(yàn)著水產(chǎn)品質(zhì)量安全執(zhí)法部門(mén)的公正性[2]。

        孔雀石綠(MalachiteGreen oxalate),(以下簡(jiǎn)寫(xiě)MG),又稱中國(guó)綠、苯胺綠、堿性孔雀石綠、品綠、鹽基塊綠,是一種合成的N-甲基二氨基三苯基甲烷類(lèi)染料(N-methylated diamino tripheny methane)。在自然界中,MG以多種形式存在,但主要是以草酸鹽或鹽酸鹽的形式存在于醋酸鹽和鹽酸鹽的混合液中??兹甘G進(jìn)入機(jī)體后,最初主要在魚(yú)的受精卵或魚(yú)苗的血清、肝、腎、肌肉、皮膚、內(nèi)臟等組織中蓄積,而后經(jīng)過(guò)生物轉(zhuǎn)化,代謝成為無(wú)色形式的脂溶性代謝產(chǎn)物無(wú)色孔雀石綠( Leucomalachite green,以下簡(jiǎn)稱LMG),被長(zhǎng)期滯留在組織中,因此在水產(chǎn)品組織中檢測(cè)到的殘留物主要是無(wú)色孔雀石綠[3]。LMG的結(jié)構(gòu)類(lèi)似于具有致癌作用的典型的芳香胺化合物,是MG染料合成的前體物,也是工業(yè)生產(chǎn)中最嚴(yán)重的污染物。

        目前,國(guó)內(nèi)外對(duì)孔雀石綠代謝方面的研究總體不多,主要是對(duì)實(shí)驗(yàn)動(dòng)物代謝的研究。Poe等研究發(fā)現(xiàn)孔雀石綠主要在受精卵和魚(yú)苗的血清、肝、腎、肌肉和其它組織中蓄積[4]。Royal等將鯰魚(yú)(Ictalurus punctatus)放在孔雀石綠藥浴中,7 d內(nèi)孔雀石綠從707 μg/kg降至17 μg/kg,無(wú)色孔雀石綠則從834 μg/kg降至451 μg/kg[5]。Alborali 等的試驗(yàn)結(jié)果表明:在魚(yú)體的肌肉皮膚中,孔雀石綠的總含量的殘留時(shí)間較長(zhǎng),不易消去[6]。

        但這些研究多集中在活體實(shí)驗(yàn)動(dòng)物的代謝上,對(duì)于冷凍樣品肌肉中的孔雀石綠殘留量影響的研究還未見(jiàn)相關(guān)報(bào)道。本實(shí)驗(yàn)將研究鯉魚(yú)、鰻鱺、鱖魚(yú)在加入不同濃度的孔雀石綠標(biāo)準(zhǔn)樣品后,在不同冷凍貯藏時(shí)間下對(duì)孔雀石綠殘留量的影響,確定影響藥物降解的關(guān)鍵因子。為農(nóng)業(yè)部實(shí)施城市例行監(jiān)督檢測(cè)、產(chǎn)地地監(jiān)督抽查、苗種監(jiān)督檢測(cè)、農(nóng)業(yè)部考核方案提供技術(shù)支持。

        1 實(shí)驗(yàn)材料

        1.1 實(shí)驗(yàn)樣品

        冷凍空白鯉魚(yú)魚(yú)糜、冷凍空白鰻鱺魚(yú)糜、冷凍空白鱖魚(yú)魚(yú)糜,由農(nóng)業(yè)部漁業(yè)環(huán)境及水產(chǎn)品質(zhì)量監(jiān)督檢驗(yàn)測(cè)試中心(天津)業(yè)務(wù)室提供。

        1.2 實(shí)驗(yàn)儀器設(shè)備

        參考文獻(xiàn):

        [1]

        鄭宗林,向磊.我國(guó)水產(chǎn)藥物使用現(xiàn)狀分析[J].水產(chǎn)養(yǎng)殖,2002(4):363-39

        [2] 穆迎春,邢麗紅,等 不同貯藏條件下孔雀石綠加標(biāo)樣品降解研究[J].中國(guó)農(nóng)學(xué)通報(bào),2012 (28):83-89

        [3] Culp SJ, et al. Metabolic changes occurring i nmice and rats fed LMG [A]. Proc 88th Ann Meeting of Am Soc for Cancer Research [C] .San Diego, 1997. 121-125

        [4] PoeW E, Wilshon R P. Absorption of malachite green by channe l catfish [J] . Prog Fish Cult, 1983 (45) : 228-229

        [5] Roybal J E, Denver C O, Pfenning A P, e t a.l Determination of malachite green and itsme tabolite, leucom a lachite green, in catfish ( Ictalurus punctatus ) tissue by liquid chrom atography with visible detection [J].J AOAC,1995( 78): 453-457

        [6] Alborali L, Sangiorgi E, Leali M. The persistence of malachite green in the edible tissue of rain bow trout (Oncorhynchus my kiss) [J]. Aquaculture,1997,32:45-60

        Different frozen storage times effect on malachite green residues in three kindy of fish muscles

        GAO Lina1,CHEN Yongping2,MA Dan2,LI Chunqing2

        (1.Tianjin Vniversity of science&Technology,Tianjin 300547;2.Quality of fishery Environment and Aquatic Products Supervision and Testing center,Ministry of Agriculture,Tianjin 300221,china

        Abstract:Study of carp, eel, mandarin fish residues of malachite green in the effects of malachite green standard samples with different concentrations and at different frozen storage time. Three kinds of matrix, eel tissues are added in the carp leucomalachite green degradation rate is the slowest, mandarin fish, three times faster, matrix added with 10 μg/kg, 50 μg/kg,100 μg/kg, colored malachite green average degradation rate were 57.9%, 48%, 23% leucomalachite green average degradation rate were 46.6%, 43.4%, 19.8%, colored malachite green is colorless malachite green fast degradation of low concentration, high concentration fast degradation. In three kinds of matrix carp, eel, mandarin fish tissues colored and colorless malachite green degradation reaction are in line with the pseudo first order reaction kinetics. Analysis may be due to eel tissue more fat, the binding mode of the degradation of colored leucomalachite green.

        Key words:Storage time; muscle; malachite green; residue

        (收稿日期:2014-06-28)

        Different frozen storage times effect on malachite green residues in three kindy of fish muscles

        GAO Lina1,CHEN Yongping2,MA Dan2,LI Chunqing2

        (1.Tianjin Vniversity of science&Technology,Tianjin 300547;2.Quality of fishery Environment and Aquatic Products Supervision and Testing center,Ministry of Agriculture,Tianjin 300221,china

        Abstract:Study of carp, eel, mandarin fish residues of malachite green in the effects of malachite green standard samples with different concentrations and at different frozen storage time. Three kinds of matrix, eel tissues are added in the carp leucomalachite green degradation rate is the slowest, mandarin fish, three times faster, matrix added with 10 μg/kg, 50 μg/kg,100 μg/kg, colored malachite green average degradation rate were 57.9%, 48%, 23% leucomalachite green average degradation rate were 46.6%, 43.4%, 19.8%, colored malachite green is colorless malachite green fast degradation of low concentration, high concentration fast degradation. In three kinds of matrix carp, eel, mandarin fish tissues colored and colorless malachite green degradation reaction are in line with the pseudo first order reaction kinetics. Analysis may be due to eel tissue more fat, the binding mode of the degradation of colored leucomalachite green.

        Key words:Storage time; muscle; malachite green; residue

        (收稿日期:2014-06-28)

        Different frozen storage times effect on malachite green residues in three kindy of fish muscles

        GAO Lina1,CHEN Yongping2,MA Dan2,LI Chunqing2

        (1.Tianjin Vniversity of science&Technology,Tianjin 300547;2.Quality of fishery Environment and Aquatic Products Supervision and Testing center,Ministry of Agriculture,Tianjin 300221,china

        Abstract:Study of carp, eel, mandarin fish residues of malachite green in the effects of malachite green standard samples with different concentrations and at different frozen storage time. Three kinds of matrix, eel tissues are added in the carp leucomalachite green degradation rate is the slowest, mandarin fish, three times faster, matrix added with 10 μg/kg, 50 μg/kg,100 μg/kg, colored malachite green average degradation rate were 57.9%, 48%, 23% leucomalachite green average degradation rate were 46.6%, 43.4%, 19.8%, colored malachite green is colorless malachite green fast degradation of low concentration, high concentration fast degradation. In three kinds of matrix carp, eel, mandarin fish tissues colored and colorless malachite green degradation reaction are in line with the pseudo first order reaction kinetics. Analysis may be due to eel tissue more fat, the binding mode of the degradation of colored leucomalachite green.

        Key words:Storage time; muscle; malachite green; residue

        (收稿日期:2014-06-28)

        猜你喜歡
        肌肉殘留
        普安銀鯽雌魚(yú)肌肉營(yíng)養(yǎng)成分分析
        關(guān)于舞蹈力量素質(zhì)訓(xùn)練的科學(xué)性研究
        戲劇之家(2017年1期)2017-02-05 13:32:48
        一種平整機(jī)濕平整吹掃裝置簡(jiǎn)析
        水產(chǎn)品中孔雀石綠殘留的毒性作用及風(fēng)險(xiǎn)評(píng)估
        磺酰脲類(lèi)除草劑分析方法的探討
        法制博覽(2016年11期)2016-11-14 10:28:42
        新疆野家雜豬肌肉組織學(xué)測(cè)定
        固相萃取—?dú)庀嗌V法檢測(cè)水體中11種農(nóng)藥殘留
        液相色譜串聯(lián)質(zhì)譜法快速測(cè)定魚(yú)肉中四聚乙醛殘留量
        国产丶欧美丶日本不卡视频| 在线亚洲国产一区二区三区| 深夜一区二区三区视频在线观看 | 国产一区二区黄色录像| 欧美人与动牲交a精品| h在线国产| 国产成人自拍视频在线观看网站 | 东京热久久综合久久88| 日本午夜免费福利视频| 国产在线欧美日韩精品一区二区| 日韩一区二区av伦理| 国产成人亚洲精品无码青| 成人性做爰aaa片免费看| 亚洲成a人片在线观看无码3d| 人妻精品无码一区二区三区| 欧美日韩国产高清| 亚洲一区二区三区在线视频| 97在线视频免费人妻| 熟妇五十路六十路息与子| 国产香蕉尹人在线视频你懂的| 中文字幕女同人妖熟女| 亚洲精品tv久久久久久久久久| 国产av日韩a∨亚洲av电影| 国产又粗又猛又黄色呦呦| 亚洲国产中文字幕一区| 韩日午夜在线资源一区二区| 国产无线乱码一区二三区| 亚洲一区二区三区久久久| 国产女人好紧好爽| 国产成人精品日本亚洲| 美女裸体无遮挡免费视频国产| 亚洲福利二区三区四区| 国产精品99精品无码视亚| 国产精品理人伦国色天香一区二区| 国产精品日本一区二区三区| 亚洲成熟女人毛毛耸耸多| 国产午夜精品久久久久免费视| 人人爽亚洲aⅴ人人爽av人人片| 日韩精品一二三区乱码| 无码少妇一区二区性色av| 无码不卡一区二区三区在线观看|