【摘要】 目的 研究雷公藤甲素對(duì)哮喘小鼠哮喘小鼠白三烯B₄、C₄表達(dá)的影響，探討其治療哮喘的機(jī)制。方法 建立小鼠卵蛋白哮喘模型，隨機(jī)分為7組，分別為對(duì)照組、地塞米松治療組及不同劑量雷公藤甲素治療組，用ELISA測(cè)定支氣管肺泡灌洗液(BALF)及肺組織勻漿中白三烯 B₄、C₄表達(dá)水平。結(jié)果 哮喘組白三烯 B₄、C₄表達(dá)明顯高于正常對(duì)照組(P<0.01)，雷公藤甲素治療組及地塞米松組明顯低于哮喘組(P<0.01)。結(jié)論 雷公藤甲素抑制哮喘氣道炎癥的機(jī)制可能與其抑制白三烯B₄、C₄表達(dá)的表達(dá)活性有關(guān)。

【關(guān)鍵詞】 雷公藤甲素;支氣管哮喘;LTB₄;LTC₄

文章編號(hào):1003-1383(2009)05-0516-02

中圖分類號(hào):R 562.25;R363文獻(xiàn)標(biāo)識(shí)碼:A

doi:10.3969/j.issn.1003-1383.2009.05.002

Effects of triptolide on the expression of TLB₄ and TLC₄ in asthmatic mice[HJ1*2][HJ]

LIU Qingyi

(Respiratory Yuebei People’s Hospital Guangdong，Shaoguan 512026)

【Abstract】 Objective To study the effect s of triptolide on the expression of TLB₄ and TLC₄ in asthmatic mouse model，and to explore the possible therapeutic mechanism of t riptolide in asthma.

Methods The asthmatic model was established by ovalbumin injection and inhalation. The mice were randomly divided into 7 groups andtreated respectively with normal saline (as control) ，dexamethasone or different dose of triptolide. The expression of TLB₄ and TLC₄ in ranchoalveolar avage fluid (BAL F) and in lung tissue was were detected by ELISA respectively.Results The expression of TLB₄ and TLC₄ in airway epithelium of asthmatic group significantly increased compared with those of controlgroup(P<0.01). The expression of TLB₄ and TLC₄ expression in mice t reated with different dose of t riptolide and dexamethson were significantly lower than those in asthmatic group(P<0.01).Con

clusion The mechanism of t riptolide inhibiting asthmatic airway inflammation may be related to its inhibiting activities of TLB₄ and TLC₄.

【Key words】 Triptolide;Asthma;LTB₄;TLC₄

支氣管哮喘是一種常見病、多發(fā)病，由于對(duì)其病因及發(fā)病機(jī)制不甚清楚，雖然采用現(xiàn)有的方法，已能有效地控制大多數(shù)病人的癥狀，但對(duì)部分病人療效欠佳，尋求和開發(fā)有效的治療哮喘的藥物對(duì)哮喘的防治具有十分重要的意義。本實(shí)驗(yàn)研究雷公藤甲素對(duì)哮喘小鼠白三烯B₄、C₄表達(dá)的影響，為開發(fā)雷公藤甲素治療哮喘在臨床上應(yīng)用提供理論依據(jù)。