蔣潔 阿米拉?阿布拉提 姚藍(lán)

摘要：目的? 觀察電針聯(lián)合兩色金雞菊提取物對(duì)糖尿病大鼠腎臟波形蛋白（Vimentin）、α-平滑肌肌動(dòng)蛋白（α-SMA）、轉(zhuǎn)化生長因子-β1（TGF-β1）及p-smad2表達(dá)的影響，探討其作用機(jī)制。方法? 將36只SD大鼠隨機(jī)分為正常組、模型組、電針組、兩色金雞菊組、電針+兩色金雞菊組和二甲雙胍組，每組6只。采用高糖高脂喂養(yǎng)聯(lián)合腹腔注射鏈脲佐菌素建立2型糖尿病模型。正常組、模型組不進(jìn)行干預(yù);電針組針刺大鼠雙側(cè)“腎俞”“脾俞”，連接電針，留針30 min;兩色金雞菊組予兩色金雞菊乙酸乙酯提取物（300 mg/kg）灌胃;電針+兩色金雞菊組予電針聯(lián)合兩色金雞菊乙酸乙酯提取物灌胃;二甲雙胍組予二甲雙胍（200 mg/kg）藥液灌胃。每日1次，治療4周。HE染色觀察腎組織形態(tài)學(xué)及纖維化程度，Western blot檢測腎組織Vimentin、α-SMA、TGF-β1及p-smad2蛋白表達(dá)。結(jié)果? HE染色結(jié)果顯示，電針組、兩色金雞菊組、電針+兩色金雞菊組和二甲雙胍組均可緩解模型大鼠腎小管擴(kuò)張，減輕腎小球皺縮現(xiàn)象，改善炎性細(xì)胞浸潤，減少基底膜增生和融合，且以電針+兩色金雞菊組改善更明顯;Western blot檢測結(jié)果顯示，電針和兩色金雞菊提取物均可降低模型大鼠腎組織Vimentin、α-SMA、TGF-β1及p-smad2蛋白表達(dá)，且以兩者聯(lián)合效果最佳。結(jié)論? 電針聯(lián)合兩色金雞菊提取物可明顯改善糖尿病大鼠腎臟纖維化，其作用機(jī)制可能與抑制TGF-β1/Smad2信號(hào)通路有關(guān)。

關(guān)鍵詞：電針;兩色金雞菊提取物;糖尿病腎病;波形蛋白;α-平滑肌肌動(dòng)蛋白;轉(zhuǎn)化生長因子-β1;Smad2;大鼠

中圖分類號(hào)：R245;285.5??? 文獻(xiàn)標(biāo)識(shí)碼：A??? 文章編號(hào)：1005-5304（2019）06-0055-04

Abstract： Objective To observe the effects of electroacupuncture combined with coreopsis tinctoria extract for expressions of Vimentin， α-SMA， TGF-β1 and p-smad2 in renal tissue of diabetic rats; To discuss its mechanism of action. Methods Totally thirty-six SD rats were randomly divided into normal group， model group， electroacupuncture group， coreopsis tinctoria group， electroacupuncture combined with coreopsis tinctoria group and metformin group， with 6 rats in each group. Type 2 DM model was established by high-fat and high-glucose diet and STZ injection intraperitoneally. Normal group and model group were given no treatment; Electroacupuncture was at the acupoints of “Pishu” and “Shenshu” once daily in electroacupuncture group for 30 min; coreopsis tinctoria group was given intragastric administration of 300 mg/kg coreopsis tinctoria extract for gavage; electroacupuncture combined with coreopsis tinctoria group was given electroacupuncture and intragastric administration of coreopsis tinctoria extract for gavage; metformin group was given intragastric administration of 200 mg/kg metformin for gavage. Histological changes and fibrosis degree of kidneys were observed by HE staining and expressions of Vimentin， α-SMA， TGF-β1 and p-smad2 were detected by Western blot. Results HE staining results indicated that

糖尿病腎?。╠iabetic nephropathy，DN）已成為終末期腎病的第2位致病因素[1]。終末期腎病一旦形成，治療極為棘手。因此，及時(shí)治療DN，防止其向終末期腎病轉(zhuǎn)化意義重大。腎臟纖維化是DN主要的病理特征之一，前期研究發(fā)現(xiàn)，兩色金雞菊提取物對(duì)高糖高脂誘導(dǎo)的大鼠腎小球系膜纖維化有明顯抑制作用[2-3]，而電針也可改善DN腎功能和超微結(jié)構(gòu)[4]。本實(shí)驗(yàn)擬將電針聯(lián)合兩色金雞菊提取物干預(yù)DN大鼠，觀察其對(duì)DN大鼠腎臟波形蛋白（Vimentin）、α-平滑肌肌動(dòng)蛋白（α-SMA）、轉(zhuǎn)化生長因子-β1（TGF-β1）及p-smad2表達(dá)的影響，明確兩者是否存在協(xié)同作用，為臨床治療DN提供依據(jù)。

1? 材料與方法