張桂芳 莊蘇

摘 要：選擇6只安裝門靜脈﹑肝靜脈和股動(dòng)脈慢性血管瘺的健康的泌乳中期薩能奶山羊作為試驗(yàn)動(dòng)物，采用2×2交叉實(shí)驗(yàn)設(shè)計(jì)，每組3只，試驗(yàn)日糧由精飼料和粗飼料組成，且精飼料和粗飼料比例分別為4∶6（高粗料組）和6∶4（高精料組），研究不同精粗比日糧對(duì)泌乳中期奶山羊肝臟氨基酸代謝的影響。結(jié)果表明，門靜脈血漿中高精料組異亮氨酸、亮氨酸、賴氨酸、蛋氨酸、組氨酸、天冬氨酸、精氨酸、支鏈氨基酸及總必需氨基酸濃度均高于高粗料組，且異亮氨酸達(dá)到顯著水平，賴氨酸達(dá)到極顯著水平，而生糖氨基酸和總氨基酸濃度低于高粗料組，差異不顯著；肝靜脈血漿中高精料組賴氨酸濃度顯著高于高粗料組，丙氨酸、甘氨酸、脯氨酸的濃度顯著低于高粗料組，其他氨基酸濃度差異不顯著；股動(dòng)脈血漿中高精料組賴氨酸濃度顯著高于高粗料組，其余氨基酸濃度無顯著差異，兩種日糧對(duì)股動(dòng)脈血漿中支鏈氨基酸、總必需氨基酸、生糖氨基酸及總氨基酸濃度的影響均不顯著。兩種日糧對(duì)門靜脈﹑肝靜脈﹑股動(dòng)脈血氨濃度無顯著影響；高精料組門靜脈﹑肝靜脈﹑股動(dòng)脈血漿中的尿素含量顯著高于高粗料組；高精料組肝靜脈血漿中胰島素、胰高血糖素、生長激素濃度及胰島素樣生長因子均高于高粗料組，但均達(dá)到顯著性差異。結(jié)論：不同精粗比日糧影響著肝臟內(nèi)的氨基酸代謝，高精料日糧可顯著增加血漿尿素水平，對(duì)血漿中相關(guān)激素?zé)o顯著影響。

關(guān)鍵詞：精粗比 氨基酸 肝臟 奶山羊

The Metabolic Pattern of Milk Protein Precursor in the Liver

Zhang Guifang Zhuang Su

（Nanjing Agricultural University）

Abstract：Six healthy mid-lactating Saanen dairy goats with catheters placed in the portal vein， a branch of the hepatic and femoral artery were used in a randomized crossover trial designed to evaluate the effect of concentrate to forage ratio on amino acid metabolism in liver. Diets content forage and concentrate， the concentrate to forage ratio were 4∶6（high forage group， HF） and 6∶4（high concentrate group， HC）. The results showed that the portal plasma concentrations of isoleucine， leucine， lysine， methionine， histidine， aspartate， arginine， branched chain amino acid and total essential amino acids were higher， especially isoleucine， lysine， while the concentrations of glucogenic amino acid and total amino acid were lower， but no markedly difference when goats fed HC diet than HF diet；In hepatic vein， the lysine concentration was markedly higher and the concentrations of alanine， glycine， proline were markedly lower when goats fed HC diet than HF diet， and other amino acids were not significantly difference； Except the concentrations of lysine， other amino acids concentration were no markedly change in femoral artery when goats fed HC diet compared with HF diet， and the concentrations of branched chain amino acid， total essential amino acids， glucogenic amino acid and total amino acid were not significantly effects. There were no marked effect on plasma NH3 concentration in portal vein， hepatic vein， femoral artery between two treated diets； The plasma urea concentration in portal vein， hepatic vein， femoral artery were markedly higher in goats fed HC diet than HF diet； The concentration of insulin， glucagon， GH， IGF-1 in hepatic vein plasma were not significantly improved when goats fed HC. Results： The metabolism of amino acids in liver was affected by concentrate to forage ratio， and higher concentrate diet significantly increased the plasma urea concentration， but hormones concentration was not affected.

Key Words：Concentrate to forage ratio； Amino acid； Liver； Dairy goats

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