李秀敏，任國棟，王新譜

(河北大學(xué) 生命科學(xué)學(xué)院，河北 保定 071002)

藍(lán)綠齒角芫菁異型觸角的掃描電鏡觀察(鞘翅目，芫菁科)

李秀敏，任國棟，王新譜

(河北大學(xué) 生命科學(xué)學(xué)院，河北 保定 071002)

藍(lán)綠齒角芫菁；觸角；感器；超微結(jié)構(gòu)；性二型

1 材料與方法

1.1研究材料

1.2研究方法

1.2.1 樣品處理及觀察

將野外采集的新鮮標(biāo)本置于解剖鏡下取下整個(gè)觸角，用超聲波清洗儀處理5 min，然后在體積分?jǐn)?shù)為50%～100%的乙醇溶液中逐級(jí)脫水，首先將處理好的觸角樣品置于超景深三維數(shù)碼顯微鏡(VHX-100型，日本基恩氏公司)下觀察觸角完整形態(tài)并拍照；之后再用導(dǎo)電膠帶將觸角固定在樣品臺(tái)上并在干燥鍋中干燥5～7 d，然后進(jìn)行離子濺射處理，將鍍金后的樣品放在中科科儀公司KYKY-2800B型掃描電子顯微鏡下觀察并記錄.

1.2.2 感器類型鑒定

觸角感器類型的鑒定參考Schneider[2]和Zacharuk[3-5]的命名系統(tǒng).

2 結(jié)果與分析

2.1形態(tài)特征

a.♀性；性

2.2感器類型

2.2.1 毛形感器(trichodea sensilla，TS)

毛形感器為分布數(shù)量最多的1類感器，根據(jù)其形狀變化分為5種亞類型:

1)毛形感器Ⅰ(TS1)：著生于表皮凹陷的基窩內(nèi)，與觸角表面形成30～60°夾角，細(xì)長，端部尖細(xì)，基部直徑約7.0 μm，長為84.0～243.0 μm，主要分布于♀性觸角的柄節(jié)和梗節(jié)周圍(圖2c)；

2.2.2 刺形感器(chaetica sensilla，CS)

其形態(tài)、著生方式同毛形感器(TS2)，但較毛形感器剛直挺立，明顯長于其他感器，基部直徑約3.0 μm，長約34.5 μm，稀疏分布于♀性的鞭節(jié)，數(shù)量較少(圖2b).

2.2.3 指形感器(digit-like sensilla，DS)

感器直立于表皮的基窩內(nèi)，形似手指，粗短，端部鈍圓，根據(jù)其形狀變化分為2種亞類型：

2.2.4 錐形感器(basiconica sensilla，BS)

分布數(shù)量僅次于毛形感器，根據(jù)其形狀變化分為3種亞類型：

2.3兩性觸角及感器分布特點(diǎn)

a，b，c，d，e.藍(lán)綠齒角芫菁♀性的觸角掃描電鏡觀察； f，g，h，i，j，k，l，m，n.藍(lán)綠齒角芫菁性的觸角掃描電鏡觀察；TS1.毛形感器Ⅰ；TS2.毛形感器Ⅱ；TS3.毛形感器Ⅲ；TS4.毛形感器Ⅳ；TS5.毛形感器Ⅴ；CS.刺形感器；BS1.錐形感器Ⅰ；BS2.錐形感器Ⅱ；BS3.錐形感器Ⅲ；DS1.指形感器Ⅰ; DS2.指形感器Ⅱ.

3 討論

藍(lán)綠齒角芫菁♀性觸角表面感器的分布與中華豆芫菁♀性表面有相似之處，DS(中華豆芫菁Epicautachinensis為BS)均大量出現(xiàn)于第3節(jié)側(cè)緣，從該節(jié)開始漸向其他鞭節(jié)的背面蔓延，并與TS相間分布[7].其DS與松褐天牛的[6]相似，推測其具有味覺功能.其末鞭節(jié)基部的TS5較粗短，與其他部位的TS形狀明顯不同，密度也較大，推測其感受信息的能力應(yīng)與其他TS有顯著的差別.

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FinestructuresoftheantennalsensillafromsexuallydimorphicantennaofCerocomaschreberi(Colepotera:Meloidae)

LIXiumin，RENGuodong，WANGXinpu

(College of Life Sciences, Hebei University, Baoding 071002,China)

There is great difference between the shape of antenna each of male and femaleCerocomaschreberiFabricius adults. The male adults have club-like antenna, while the shape of female adult’s antenna is irregular. This paper described this sexual dimorphism of the shape of the antenna ofC.schreberiFabricius adults based on the scanning electron microscopic observation and analyses. It showed that totally 11 types of sensilia, which were grouped into 4 categories, were observed, including 5 trichiform sensilla (TS), 3 chaetiform sensilla (CS), 1 digit-like sensillum (DS), 1 basiconic sensillum (BS). The BS3, TS2 and DS1 type are present in two sexes. Most of these were trichoform sensilla and digit-like sensilla. Significant difference in types, amount and distribution of sensilla was found between two sexes.

CerocomaschreberiFabricius; antenna; sensila; ultra-structure; sexual dimorphism

10.3969/j.issn.1000-1565.2013.04.013

2012-03-02

國家自然科學(xué)基金資助項(xiàng)目(31071956)；河北省自然科學(xué)基金資助項(xiàng)目(C2012201142，C2011201086)；河北省無脊椎系統(tǒng)學(xué)與應(yīng)用重點(diǎn)實(shí)驗(yàn)室資助項(xiàng)目(HBIS09001)

李秀敏(1978-)，女，河北滄州人，河北大學(xué)講師，主要從事昆蟲系統(tǒng)學(xué)研究.E-mail:lixiumin@hbu.edu.cn

任國棟(1955-)，男，寧夏中寧人，河北大學(xué)教授，博士生導(dǎo)師，主要從事昆蟲學(xué)研究.

E-mail:gdren@hbu.edu.cn

Q964

A

1000-1565(2013)04-0408-05

(責(zé)任編輯趙藏賞)