Bao-Chi Liu, Ming-Rong Cheng, Lin Lang, Lei Li, Yan-Hui Si, Ai-Jun Li, Qing Xu, Hui Zhang

Abstract

Key Words: Autologous bone marrow; Splenectomy; Cell therapy; Cirrhosis; Cellular immunity

INTRODUCTION

Cirrhosis is the end stage of liver fibrosis and has several causes. In the global ranking of causes of death in 2012, cirrhosis ranked 14th[1], and drug treatment was not effective. At present, liver transplantation is still the most effective treatment for advanced liver disease. However, due to the lack of donor livers and high costs, there is an urgent need to find a safe and effective alternative that can be widely used[2]. Cell therapy has achieved outstanding results in basic and clinical research and is a promising new treatment. In animal experiments, bone marrow stem cells (BMSCs) can be transformed into hepatic oval cells, hepatocytes and bile duct cells in the liver, which play an important role in the repair of liver damage[3]. In a clinical experiment, it was found that after transplantation of male bone marrow cells (BMCs) to female patients, Y-chromosome-positive hepatocytes were detected in the liver, and it was confirmed that BMSCs can be transformed into hepatocytes[4]. Liver-derived liver stem cells have a positive contribution to hepatocyte regeneration. Luet al[5] induced hepatocyte apoptosis by targeted deletion ofMdm2, and then transplanted liver progenitor cells into mouse liver. The transplanted liver progenitor cells differentiated into hepatocytes and bile duct cells, significantly improving the structure and function of the damaged liver.

Several studies have reported positive effects of BMCs transplantation for treatment of decompensated liver cirrhosis (DLC). Most studies used BMCs, peripheral blood hematopoietic stem cells and umbilical cord blood stem cells. BMCs were separated by gradient centrifugation, and the suspension of BMCs was injected into the liver through the hepatic artery by interventional methods. Liver function was repaired, but the specific mechanism is still not clear[6]. The anti-DLC effect of autologous BMSCs has been established in animal models[7]. In addition, clinical trials have shown that autologous BMSC transplantation can quickly improve liver function without obvious side effects. However, there are not many clinical trials about autologous BMSCs for cirrhosis, and there is no unified treatment plan[8,9]. In a previous study, BMSCs were used to treat human immunodeficiency virus (HIV) patients with DLC, and achieved good results. This treatment method has the following advantages: BMSCs do not need to be centrifuged, and are reproducible, nonimmunogenic, and free of graft-versus-host disease[10]. Therefore, autologous BMSCs have received much attention in basic and clinical research, and are increasingly used in clinical treatment of various diseases[11-13]. BMSCs are transplanted to the liver mainly through the hepatic artery, portal vein and peripheral vein. This has a significant effect on DLC, is safe and feasible, and can significantly improve the clinical symptoms and liver function[14-16]. It is readily accepted by DLC patients. Other transplantation routes such as intrasplenic transplantation, intraperitoneal and peripheral vein transplantation are commonly used clinically. In a study of four patients with DLC who were treated with peripheral intravenous injection of autologous mesenchymal stem cells, after 2 years of follow-up, it was found that the scores of the end-stage liver disease model in two patients were significantly improved[17], but the number of cases was too small. The mechanism by which stem cells repair liver cells through systemic blood circulation is still unclear, and no effective induction method has been found to orient stem cells to migrate and home to the target organ. This transplantation method is the most applied method in liver cirrhosis.

同時(shí)，醫(yī)院內(nèi)部各部門壓力重重，省醫(yī)院招投標(biāo)工作原分散于各部門：設(shè)備采購(gòu)歸設(shè)備材料科管、維保采購(gòu)管理歸機(jī)電維修科管，于是設(shè)備采購(gòu)管買不管修；維修科科長(zhǎng)因直面所有廠家，壓力大，且沒(méi)有維保購(gòu)買及管理的主動(dòng)權(quán)……

Due to the rich blood supply and nutrients in the portal vein, stem cells stay in the liver sinusoids for a long time, with good selectivity distribution, and the portal system contains high concentrations of hepatotropic cytokines, which are important for the survival and growth of BMCs that are returned to the liver. In previous clinical trials surgery was performed to insert an infusion port into the right omental vein (ROV), autologous BMCs were returned through the infusion port, and good results were observed in the treatment of HIV patients with DLC[18]. However, it remains unclear whether splenectomy has an effect on patient outcomes. In this study, the efficacy of autologous bone marrow infusion (ABMI) combined with splenectomy was observed in the treatment of patients with DLC.

MATERIALS AND METHODS

Patients

This was a retrospective analysis of 83 patients with DLC who received ABMI, including 52 males and 31 females, aged 27-75 years, with an average age of 47.53 ± 8.82 years from January 2016 to December 2018 from Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai Dongfang Hospital Affiliated to Tongji University, Shanghai Dongfang Hepatobiliary Surgery Hospital Affiliated to Naval Medical University, and Renji Hospital Affiliated to Shanghai Jiao Tong University. There were 60 cases of hepatitis B cirrhosis, four of alcoholic cirrhosis, 11 of hepatitis C cirrhosis and eight cases of schistosomiasis cirrhosis. Child–Pugh classification was grade B in 77 cases and grade C in six.

Inclusion was in accordance with the diagnostic criteria for DLC[19]: (1) Computed tomography (CT), color Doppler ultrasound, or liver biopsy suggested the formation of liver cirrhosis; (2) liver cirrhosis was diagnosed by liver hardness scan; (3) albumin level < 35 g in liver function; (4) gastroscopy showed signs of esophageal and gastric varices; (5) platelet count < 1011/L; (6) esophageal and gastric varices; (7) prothrombin time was longer than normal for 3 s; and (8) ascites formation. If any three items in (1)-(5) were met and any item in (6)-(8), it was possible to diagnose DLC. Exclusion criteria were: (1) Age < 18 years; (2) pregnant and breastfeeding women; (3) malignant tumors of the liver or other organs; (4) spontaneous peritonitis or active gastrointestinal bleeding; (5) patients who could not tolerate the treatment, such as severe those with heart disease and pulmonary insufficiency; (6) hormone therapy; and (7) intellectual disability or mental illness.

Informed consent was signed by all patients and the study was approved by the Ethics Committee of the Shanghai Public Health Clinical Center (2013-030).

Treatment

Conventional liver protection and diuresis were used for all patients. In addition, viral liver cirrhosis was treated with anti-hepatitis B or C drugs.

According to whether splenectomy was performed during the operation, the patients were divided into an intervention group (43 cases) and control group (40 cases). The control group was treated with ABMI through the ROV, and the intervention group underwent splenectomy in addition to ABMI.

Under general anesthesia, a midline incision was made in the upper abdomen, the ascites was removed after entering the abdominal cavity, the spleen was fully exposed and then the spleen was removed (in the intervention group). The infusion port was embedded in the ROV. During the operation, 40 mL of bone marrow was extracted from the anterior superior iliac spine by puncture, and 40 mL of ABM (without washing, filtration and concentration) was slowly injected with a syringe through the puncture window of the infusion port, and entered the portal vein through the ROV. Saline (5 mL) was injected into the infusion port to prevent coagulation. At 1 and 3 mo after the operation, 40 mL of ABM was infused once again through the infusion port. Five milliliters of cubital venous blood were drawn before surgery, at 1, 3, 6 and 12 mo after ABMI, placed in an anticoagulation tube, and allowed to stand at room temperature for 30 min. The samples were centrifuged centrifugal radius was 9 cm, centrifugal speed was 3000 rpm, 15 min (Tengying Machinery Manufacturing Co., Ltd., Zhangjiagang, China), and supernatants were collected and stored at -80 °C.

Biochemical analysis

Serum total bilirubin (TB), serum albumin and creatinine levels were detected using an AU5800 automatic biochemical analyzer (Beckman Coulter Co. Ltd., CA, United States). Prothrombin time (PT) and international normalized ratio (INR) were measured by CA-500 automatic coagulation analyzer (Sysmex Corporation, Kobe, Japan) using the coagulation index detection kit. The white blood cell (WBC) and platelet counts and hemoglobin level were detected by automatic blood analyzer (Mindray BC-5000, Shenzhen, China).

Flow cytometry

FACS Calibur flow cytometer, CD4-FITC/CD8-PE, TruCOUNT absolute counting tube, four-color fluorescent standard microspheres, and FACS hemolysin (10 ×) were the products of BD Company in the United States. Multiset tri-color reagent (20 μL) and 50 μL whole blood were added to a TruCOUNT absolute counting tube, mixed thoroughly, and placed in the dark for 15 min. Then, 450 μL of FACS hemolysin (10 ×) was added, mixed well, and placed in the dark for 15 min. After sample preparation, the Multiset program was run on the computer immediately for detection, and the absolute counts of CD4+and CD8+T cells in the total T cells were analyzed.

Determination of ascites

Inadomiet al[20] reported a method of measuring the volume of ascites with ultrasound in 1996. Two variables were observed: Abdominal circumference and maximum ascites depth. Specific operation: Instruct the patient to lie on his back and lie on the stomach, and we measured the abdominal circumference (C) around the umbilicus, and then the patient changed to the prone position. The ultrasonic probe probed the maximum depth of ascites at the umbilical circumference (d), that is, the maximum vertical distance from the interface of the floating intestinal loop to the probe. We used the following formula to calculate the amount of ascites: r = C/2π; V (volume of ascites) = 1/3 [πd2(3r-d)] (Note: r is radius, π is constant).

Model for end-stage liver disease

Model for end-stage liver disease (MELD) score is often used as an indicator of liver function. The formula was MELD score[21] = 3.78 × ln[TB (μmol/L)] + 11.2 × ln(INR) + 9.6 × ln[creatinine (mmol/L)] + 6.4 × (etiology: Biliary or alcoholic was 0, and other diseases were 1).

Statistical analysis

SPSS 19.0 was used for statistical analysis. The normal distribution was tested using the Shapiro-Wilk test. The measurement data conforming to the normal distribution were expressed as mean ± SD. Before and after treatment, the pairedttest was used for comparison of the intervention group and control group. The data with non-normal distribution were expressed in M (P25, P75), using the Mann-Whitney test. Numerical data were used to describe the percent, using theχ2test.P< 0.05 was considered statistically significant. The test standard was α = 0.05.

RESULTS

Comparison of general baseline data between control and intervention groups

There was no significant difference in gender, age, etiology of liver cirrhosis and Child–Pugh grading between the intervention group and control group (P> 0.05), and the baseline data of the two groups were comparable (Table 1).

Comparison of postoperative complications

In the intervention group, 43 patients underwent splenectomy and had an infusion port placed in the right gastroepiploic vein. Three patients (all Child-Pugh grade B before surgery) had liver failure due to oozing blood in the splenectomy wound, and died within 3 d after surgery. The surgery-related fatality rate was 6.98%. In the control group, 40 patients had an infusion port placed in the right gastroepiploic vein, and two patients (1 Child-Pugh grade B and 1 grade C before surgery) died of liver failure caused by gastrointestinal bleeding after surgery, and the fatality rate was 5.0%. There was no significant difference in the fatality rate between the two groups after surgery (χ2= 0.007,P> 0.05). In the intervention group, two cases were Child-Pugh grade C. Due to emergency surgery for gastrointestinal bleeding, the portal vein pressure was high. If splenectomy is not performed, it may recur to bleed after surgery, so splenectomy was performed. After 1-year follow-up, both patients showed good improvement in liver function, similar to that in the Child–Pugh grade B patients.

Liver synthetic and secretory functions after surgery

Figure 1A and Table 2 show that there was no significant difference in serum PT levels between the control and intervention groups before ABMI (P> 0.05). After ABMI, serum PT in both groups was significantly lower than before ABMI (P< 0.01), but there was no significant difference between the two groups at each time point (P> 0.05).

Figure 1B and Table 3 show that there was no significant difference in serum albumin levels between the two groups before ABMI (P> 0.05). After ABMI, albumin level in both groups was significantly higher than before ABMI (P< 0.01), but there was no significant difference between the two groups at each time point (P> 0.05).

Figure 1C and Table 4 show that there was no significant difference in serum TB level between the two groups before ABMI (P> 0.05). After ABMI, serum TB level in both groups was significantly lower than before ABMI (P< 0.01), but there was no significant difference between the two groups at each time point (P> 0.05).

Figure 1D and Table 5 showed that there was no significant difference in ascites volume before ABMI between the two groups (P> 0.05). After ABMI, ascites volume in both groups was significantly lower than before ABMI (P< 0.01), but there was no significant difference between the two groups at each time point (P> 0.05).

Figure 1E and Table 6 showed that there was no significant difference in MELD score before ABMI between the two groups (P> 0.05). After ABMI, MELD score in both groups was significantly lower than before ABMI (P< 0.01), but there was no significant difference between the two groups at each time point (P> 0.05).

Hematopoietic function before and after ABMI

Figure 2A and Table 7 show that there was no significant difference in WBC count between the control and intervention groups before ABMI (P> 0.05). After ABMI, WBC count in both groups was significantly higher than before ABMI (P< 0.01). The increase in the intervention group at each time point was significantly higher than in the control group (P< 0.01).

Table 1 Comparison of general baseline data between control and intervention groups

Table 2 Serum prothrombin time levels in control and intervention groups before and after autologous bone marrow infusion

Table 3 Serum albumin levels in control and intervention groups before and after autologous bone marrow infusion (mean ± SD, g/L)

Table 4 The serum total bilirubin levels in control and intervention groups before and after autologous bone marrow infusion

Figure 2B and Table 8 show that there was no significant difference in serum platelet count between the two groups before ABMI (P> 0.05). After ABMI, platelet count in both groups was significantly higher than before ABMI (P< 0.01). The increase in the intervention group at each time point was significantly higher than in the control group (P< 0.01).

Table 5 Ascites volume in control and intervention groups before and after autologous bone marrow infusion

Table 6 Comparison of model for end-stage liver disease scores in two groups before and after autologous bone marrow infusion

Table 7 Comparison of white blood cell in control and intervention groups before and after autologous bone marrow infusion

Table 8 Comparison of platelet counts in control and intervention groups before and after autologous bone marrow infusion

Figure 2C and Table 9 showed that there was no significant difference in serum hemoglobin level between the two groups before ABMI (P> 0.05). Serum hemoglobin level in both groups at 3, 6 and 12 mo after ABMI was significantly higher than before ABMI (P< 0.01). The increase at 6 and 12 mo in the intervention group was significantly higher than in the control group (P< 0.05 andP< 0.01).

Comparison of CD4+ and CD8+ T cell counts before and after ABMI

Figure 3A and Table 10 show that there was no significant difference in CD4+T cell count before ABMI between the control and intervention groups (P> 0.05). After ABMI, CD4+T cell count in the intervention group at 1, 3, 6 and 12 mo, and in the control group at 3, 6 and 12 mo was significantly higher than before ABMI (P< 0.01), but there was not significant difference between the two groups at each time point (P> 0.05).

Table 9 Comparison of hemoglobin levels in control and intervention groups before and after autologous bone marrow infusion

Table 10 Comparison of CD4+ T cell count in control and intervention groups before and after autologous bone marrow infusion

Figure 3B and Table 11 showed that there was no significant difference in CD8+T cell count before ABMI between the two groups (P> 0.05). After ABMI, CD8+T cell count in the intervention group at 1, 3, 6 and 12 mo, and in the control group at 1, 3 and 6 mo were significantly higher than before ABMI (P< 0.01), but there was no significant difference between the two groups at each time point (P> 0.05).

DISCUSSION

Stem cells are not highly differentiated and have the potential to regenerate various tissues and organs in the human body. They are called universal cells in the medical field. Under certain conditions, they can differentiate into multiple functional cells. According to different differentiation potentials, they can be divided into four categories[22]: (1) Totipotent stem cells that can develop into independent individuals; (2) multipotent stem cells that are the descendants of universal stem cells; they cannot develop into individuals, but they can develop into multiple tissues; (3) pluripotent stem cells that can only differentiate into cells of specific groups, such as specific tissues or organs; and (4) unipotent stem cells that can only produce one cell type and have a self-updating property. BMSCs are a type of stem cells with multidirectional differentiation potential and self-renewal. They can differentiate into specific tissues under special circumstances, including liver cells and cardiomyocytes. BMSC transplantation, as a new technology for repairing regenerated damaged organs, has become a research hotspot for stem cell transplantation due to the advantages of convenient collection, low rejection, safety and reliability, and low cost[23,24]. BMSCs can replace damaged hepatocytes by inducing differentiation into hepatocytes in severe liver disease, improve function of the damaged liver, and bring new hope for the treatment of cirrhosis[25,26]. In our previous study, ABMI for patients with DLC and acquired immunodeficiency syndrome significantly prolonged survival. ABMI has the following advantages over traditional treatment: (1) It is simple and easy to collect autologous bone marrow, and there is no shortage of donors; (2) there is usually no immune rejection; (3) it is safe, with almost no adverse reactions, exogenous pollution, and disease transmission; and (4) it has remarkable efficacy at a low cost. Whether splenectomy has an impact on the efficacy of patients with DLC remains unclear. In this study, regardless of whether the spleen was removed, serum PT, TB, and ascites volume after ABMI were significantly lower than before ABMI, and albumin levels were significantly higher. TB is an index for evaluating liver reserve function, albumin is an important index for evaluating liver synthetic function, and PT is an index for evaluating the degree of liver cell necrosis. If these indexes increase or decrease, it indicates that liver function is damaged. The possible mechanism is that after ABMI through the ROV, BMSCs can secrete a large number of different growth factors themselves, stimulate damaged liver cells, promote production of hepatocyte growth factors, promote BMSC differentiation, and exert antiapoptosis, so that the fibrotic liver regenerates and repairs liver function[27,28]. Liver function improvement also improves coagulation function and reduces the risk of spontaneous bleeding. Most DLC patients still have bleeding in the digestive tract after endoscopic ligation and compression of the three-lumen two-balloon tube in other hospitals, and it is often difficult to undergo further treatment. For patients with gastrointestinal bleeding, conservative treatment such as blood transfusion, hemostasis, and hepatoprotective diuresis can alleviate the condition. We adopt elective splenectomy + infusion port placement. For patients who fail conservative treatment, emergency surgery is required. If the liver function is above Child-Pugh grade B, splenectomy and infusion port placement can be selected. Child-Pugh grade C increases the risk of postoperative complications. It is necessary to consider the comprehensive conditions and the pros and cons of splenectomy in patients with DLC. If conditions permit, splenectomy can be considered. In the intervention group, there were two DLC patients with acute gastrointestinal hemorrhage with Child-Pugh grade C. If splenectomy is not performed, postoperative bleeding may occur. Therefore, splenectomy was still selected during the operation. After 1-year follow-up, the Child-Pugh grade C patients achieved the same curative effect as the patients with grade B, so liver function classification is not a barrier for choosing splenectomy, and we will expand the number of DLC patients in our future clinical work. In DLC patients, splenectomy can be selected if one of the following three indications was met: (1) Giant spleen, which affects the daily life of the patient; (2) the hypersplenism is serious; and (3) preventing bleeding caused by portal vein pressure. This study showed that splenectomy does not increase the complications caused by surgery, and can improve the symptoms caused by hypersplenism. We found that there was no significant difference between the surgical mortality of the intervention and control groups. The intervention group mainly suffered from bleeding on the wound surface due to splenectomy, while the control group mainly suffered from gastrointestinal bleeding after surgery. There was no obvious relationship between gastrointestinal bleeding and ABMI. The infusion volume in this group was approximately 40 mL, the infusion speed was slow, and the possibility of gastrointestinal bleeding was small. So the bleeding is mainly related to the stress of surgical trauma.

Table 11 Comparison of CD8+ T cell count in control and intervention groups before and after autologous bone marrow infusion

In this study, the surgical complications in both groups had some relationship with coagulation dysfunction, and after ABMI, the coagulation function gradually improved. Ascites is an important indicator of liver dysfunction. After ABMI, the levels of ascites in both groups were significantly less than before ABMI, indicating that both treatments had a significant effect on improving liver function. There was no significant difference in PT, albumin, ascites and MELD score between the two groups at each time point after ABMI, indicating that ABMI improved liver synthetic and secretory functions and this was not related to splenectomy. The MELD score estimated liver disease severity according to the three parameters of INR, TB and creatinine reflecting not only liver injury but also kidney function. Funget al[21] studied patients with acute onset of chronic hepatitis B and found that MELD score accurately predicted short-term mortality of patients. When MELD score was ≥ 10.51, the risk of death increased 3.057 times, which has clinical significance for guiding assessment of later follow-up and prognosis.

There are multiple reasons for anemia in patients with liver cirrhosis. The deposition of fibrous tissue in the liver leads to impaired portal vein blood flow, thereby limiting liver hyperplasia[29,30]. Long-term anorexia leads to malnutrition, which makes the intake of iron inadequate, and patients generally have portal hypertension gastrointestinal disease, which reduces iron absorption. Chronic blood loss in turn causes the loss of iron to exceed the amount of iron supplementation, and the stored iron decreases. The possible mechanisms are: (1) Hepatitis B virus causes bone marrow hematopoietic stem cells and hematopoietic regulatory factors to fail to function normally[31]; (2) hematopoietic stem cells proliferate, maintain stemness, or are blocked for differentiated into myeloid stem cells and lymphoid stem cells[32]; (3) hepatitis B virus infection causes immune damage, resulting in hematopoietic stem cell apoptosis, leading to bone marrow hematopoietic failure[33]; and (4) virus-mediated autoimmune abnormalities cause liver dysfunction, reduce degradation of toxic metabolites, resulting in ischemia and necrosis of pluripotent stem cells, proliferation of hematopoietic stem cells is inhibited, and peripheral blood cell production is reduced from the source[34]. Cirrhosis causes portal hypertension, increased splenic vein pressure, splenic congestion and hypersplenism, leading to increased destruction of peripheral blood cells. In this study, after ABMI through the ROV in two groups, liver function was improved, and the levels of WBC count, hemoglobin, and platelet count were higher than before ABMI, but the increases in the intervention group were more obvious than in the control group, indicating that splenectomy can relieve hypersplenism caused by liver cirrhosis. Peripheral blood T cell subsets mediate the adaptive cellular immune response, which is often regarded as an indicator of immune function in clinical practice. In particular, the immune response of virusspecific T cells has an important effect and regulation in liver pathology and virus clearance[35]. CD4+T cells are helper T cells, CD8+T cells are cytotoxic T cells, and they secrete different cytokines to exert immune effects[36]. In a previous study, after ABMI through the ROV in the treatment of HIV patients with DLC[18], the CD8+and CD4+T cells showed obvious changes, and we found that CD8+and CD4+also changed in non-HIV patients with DLC, so we found that ABMI had an impact on immune function, so we retained these two indicators in the present study. We found that after ABMI, the CD4+T cell count at each time point in the control and intervention groups was significantly higher than before ABMI, but there was no significant difference between the two groups. The CD8+T cell count in the control group showed a significant increase after ABMI at 1 and 6 mo, but decreased at 12 mo, which was not significantly different from that before ABMI. In the intervention group, the CD8+T cell count continued to increase after ABMI at 3, 6 and 12 mo, and there was no obvious decrease in CD8+T cell count, indicating that splenectomy can promote continuous increase of CD8+T cell count. Without removing the spleen, there was a transient increase in CD8+T cell count, and after 12 mo of ABMI the CD8+T cell count was continuously decreased in the spleen because of hypersplenism.

Figure 1 Scatter plots of serum prothrombin time, albumin, total bilirubin, ascites volume and model for end-stage liver disease score before and after autologous bone marrow infusion in control and intervention groups. A: Scatter plot of serum prothrombin time levels before and after autologous bone marrow infusion (ABMI) in both groups; B: Scatter plot of serum albumin levels before and after ABMI in both groups; C: Scatter plot of serum total bilirubin levels before and after ABMI in both groups; D: Scatter plot of ascites volume before and after ABMI in both groups; E: Scatter plot of model for endstage liver disease score before and after ABMI in both groups. Compared with before ABMI, aP < 0.01. PT: Prothrombin time; TB: Total bilirubin; MELD: Model for end-stage liver disease; ALB: Albumin.

This study had some limitations. The follow-up time was too short to analyze the 5-year survival of patients. The specific mechanism of nucleated cells in treatment of DLC is still unclear. In order to address the above shortcomings, we will further follow up the patients, extend the follow-up time to > 5 years. We will also carry out animal experiments to further explore the mechanism of nucleated cells in treatment of DLC.

CONCLUSION

ABMI through the ROV in patients with DLC can significantly improve liver synthetic and secretory functions but cannot relieve hypersplenism. ABMI combined with splenectomy can improve liver function and alleviate hypersplenism. However, splenectomy in patients with DLC has a higher risk of surgery-related complications.

Figure 2 Scatter plots of white blood cell, platelet and hemoglobin levels before and after autologous bone marrow infusion in control and intervention groups. A: Scatter plot of white blood cell count before and after autologous bone marrow infusion (ABMI) in both groups; B: Scatter plot of platelet count before and after ABMI in both groups; C: Scatter plot of hemoglobin level before and after ABMI in both groups. Compared with before ABMI, aP < 0.01;compared with control group, bP < 0.05, cP < 0.01. WBC: White blood cell; PLT: Platelet; Hgb: Hemoglobin.

Figure 3 Scatter plot of CD4+ T and CD8+ T counts in control and intervention groups before and after autologous bone marrow infusion. A:Scatter plot of CD4+ T cell count in both groups before and after autologous bone marrow infusion (ABMI); B: Scatter plot of CD8+ T cell count in both groups before and after ABMI. Compared with before ABMI, aP < 0.01.

ARTICLE HIGHLIGHTS

Research motivation

The main purpose of this study was to determine the efficacy of ABMI combined with splenectomy in the treatment of DLC, to clarify the impact of splenectomy on liver and bone marrow function, and to provide a basis for routine splenectomy in patients with DLC.

Research objectives

To clarify the efficacy of ABMI combined with splenectomy in the treatment of DLC, and the impact of splenectomy on liver and bone marrow function, so as to provide basis for rational treatment of DLC.

Research methods

In this study, ABMI combined with splenectomy was used to treat DLC, and the impact of splenectomy on liver and bone marrow function was observed. These common clinical indicators (such as the prothrombin time, serum total bilirubin, ascites volume, white blood cell and platelets counts and so on.) were used to evaluate liver and bone marrow function, which were easy to be popularized in clinic.

Research results

This study shows that ABMI combined with splenectomy is effective in the treatment of DLC, which can help to recover liver and bone marrow function, and alleviate hypersplenism. However, the sample size of this study is small and the follow-up time is short, which needs to be further improved in future studies.

Research conclusions

ABMI combined with splenectomy is a new method for the treatment of DLC, which provides a theoretical basis for the treatment of other chronic diseases.

Research perspectives

Whether ABMI is suitable for other diseases such as osteoarthropathy, cerebral infarction sequelae, diabetes and other chronic diseases, they still need further study.

ACKNOWLEDGEMENTS

We would like to thank Chinese Academy of Sciences Experimental Platform for providing technological support.

FOOTNOTES

Author contributions:Liu BC outlined the content and reviewed the manuscript; Cheng MR reviewed the literature and wrote the manuscript; Lang L, Li L and Si YH performed this experimental work and analyzed the data; Li AJ, Xu Q and Zhang H participated in the experiments; Liu BC and Cheng MR contributed equally as joint first authors.

Supported byShenkang Hospital Development Center of Shanghai, China, No. SHDC12016129.

Institutional review board statement:This study was approved by the Ethics Committee of the Shanghai Public Health Clinical Center (2013-030).

Informed consent statement:All study participants, or their legal guardian, provided informed written consent prior to study enrollment.

Conflict-of-interest statement:The authors declare that they have no competing interests.

Data sharing statement:No additional data are available.

Open-Access:This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/

Country/Territory of origin:China

ORCID number:Bao-Chi Liu 0000-0001-9051-1223; Lin Lang 0000-0002-2891-408X; Ai-Jun Li 0000-0002-2891-4079; Qing Xu 0000-0012-2891-407X; Hui Zhang 0000-0092-2891-407X.

S-Editor:Yan JP

L-Editor:A

P-Editor:Yu HG