程茜南 李東寧

[摘要] 目的 探討慢性自發(fā)性蕁麻疹（CSU）患者外周血調(diào)節(jié)性T細(xì)胞（Treg）及輔助性T細(xì)胞1（Th1）相關(guān)細(xì)胞因子白細(xì)胞介素35（IL-35）及白細(xì)胞介素18（IL-18）的水平變化。 方法 選取2019年6月至2020年10月就診于本院皮膚科的慢性自發(fā)性蕁麻疹患者50例和同時(shí)期體檢中心健康者30名，采用酶聯(lián)免疫吸附法（ELISA）檢測(cè)外周血IL-18和IL-35表達(dá)水平，分析其水平差異及與臨床癥狀、體征積分（UAS）之間的相關(guān)性。 結(jié)果 CSU患者外周血IL-18表達(dá)水平為（125.75±19.10）pg/mL，較健康對(duì)照組的（68.96±10.65）pg/mL顯著升高，與臨床癥狀、體征積分呈正相關(guān)，差異有統(tǒng)計(jì)學(xué)意義（P<0.05）;CSU患者外周血IL-35表達(dá)水平為（41.86±6.13）pg/mL，較健康對(duì)照組的（77.38±13.19）pg/mL顯著降低，與臨床癥狀、體征積分呈負(fù)相關(guān)，差異有統(tǒng)計(jì)學(xué)意義（P<0.05）。 結(jié)論 IL-35及IL-18在CSU發(fā)病中可能發(fā)揮一定作用，可能為CSU的臨床評(píng)價(jià)和治療帶來(lái)新思路。

[關(guān)鍵詞] CSU;IL-18;IL-35;相關(guān)性

[中圖分類號(hào)] R758.2? ? ? ? ? [文獻(xiàn)標(biāo)識(shí)碼] A? ? ? ? ? [文章編號(hào)] 1673-9701（2021）24-0010-04

Expression and significance of IL-18 and IL-35 in peripheral blood of patients with chronic spontaneous urticaria

CHENG Qiannan? ?LI Dongning

Department of Dermatology， the First Affiliated Hospital of Jinzhou Medical University， Jinzhou? ?121000， China

[Abstract] Objective To explore the changes of the peripheral blood regulatory T cells （Treg） and helper T cell 1 （Th1） related cytokine interleukin-35 （IL-35） and interleukin-18 （IL-18） in patients with chronic spontaneous urticaria （CSU）. Methods Fifty patients with chronic spontaneous urticaria in the dermatology department of our hospital and 30 healthy patients in the physical examination center from June 2019 to October 2020 were selected. Enzyme-linked immunosorbent assay （ELISA） was used to detect the expression levels of IL-18 and IL-35 in peripheral blood. The differences in IL-18 and IL-35 levels and their correlation with clinical symptoms and physical signs （UAS） were analyzed. Results The expression level of IL-18 in peripheral blood of CSU patients was （125.75±19.10）pg/mL，which was significantly higher than （68.96±10.65）pg/mL of the healthy control group， which was positively correlated with clinical symptoms and signs， and the difference was statistically significant（P<0.05）. The expression level of IL-35 in peripheral blood of CSU patients was （41.86±6.13）pg/mL， significantly lower than （77.38±13.19） pg/mL of the healthy control group， which was negatively correlated with clinical symptoms and physical signs， and the difference was statistically significant（P<0.05）. Conclusion IL-35 and IL-18 may play a role in the pathogenesis of CSU， which may bring new ideas for the clinical evaluation and treatment of CSU

[Key words] CSU; IL-18; IL-35; Correlation

慢性自發(fā)性蕁麻疹（Chronic spontaneous urticaria，CSU）是皮膚科門診高度流行的一種變態(tài)反應(yīng)性疾病，由多種炎癥細(xì)胞及因子參與并累及到皮膚黏膜。有研究表明，細(xì)胞失衡與CSU的發(fā)生發(fā)展有密切關(guān)系。特別CD4+ T淋巴細(xì)胞。其中包含輔助性T淋巴細(xì)胞（Th細(xì)胞）和調(diào)節(jié)性T淋巴細(xì)胞（Treg細(xì)胞），而Th細(xì)胞包括（Th1淋巴細(xì)胞亞群、Th2淋巴細(xì)胞亞群、Th17淋巴細(xì)胞亞群），并將研究熱點(diǎn)集中于Th1與Th2細(xì)胞、Treg細(xì)胞和Th17細(xì)胞間的失衡。白細(xì)胞介素-35（Interleukin，IL-35）屬于IL-12家族，于2007年由Collison等發(fā)現(xiàn)并命名，其表達(dá)與分泌的方式卻僅限于Treg細(xì)胞，且有免疫抑制功能。白細(xì)胞介素-18（Interleukin，IL-18）是一種Th1細(xì)胞相關(guān)的IL-1家族細(xì)胞因子，是一種多向性免疫調(diào)節(jié)因子。IL-18通過(guò)誘導(dǎo)干擾素（IFN-γ）而發(fā)揮較強(qiáng)的促炎作用。目前，關(guān)于CSU與IL-35及IL-18的相關(guān)性研究甚少。本研究通過(guò)對(duì)CSU患者外周血IL-35及IL-18表達(dá)水平的檢測(cè)，分析IL-35及IL-18在CSU發(fā)生發(fā)展的可能機(jī)制，現(xiàn)報(bào)道如下。