Maurizio Salvadori,Aris Tsalouchos

Abstract

Key words:Kidney evaluation;Pre-transplant biopsies;Kidney donor evaluation;Kidney risk profile index;Omic technologies;Deceased donor score;Donor risk score

INTRODUCTION

To date,organ shortage represents one of the major limitations to the development of kidney transplantation.

To increase the donor pool many transplant programs accept kidneys from the socalled extended criteria donors (ECDs)[1,2].Kidneys from the ECD pool are known to have worse outcomes in recipients with a higher rate of delayed graft function (DGF),primary non function (PNF),and reduced function of the allograft and reduced graft survival[3].The main challenge is to evaluate such kidneys before transplantation either for a better and fair allocation or for discarding the kidney in the case of a very poor evaluation of the offered kidney.

Several factors related to the donors are known to influence the post-transplant outcomes.Figure 1 identifies which donor,procurement and graft characteristics principally influence the outcomes.They may be divided into clinical and histological factors and factors related to the donor and related to the offered kidney and to the procurement management.

Historically,the evaluation of the kidneys from ECDs has been made histologically by the so-called zero-time biopsy[4],by clinical evaluation of the donor by different kidney allocation scores or by a combination of histological and clinical parameters.

Additionally,it should be highlighted that the need of a clear evaluation of the “so called” marginal donors became a must with the increased use of such kidneys.With time the experience documented that several kidneys from ECD pool performed well,while other kidneys labeled as standard criteria donors (SCD) did not perform well.Hence,the opportunity of a safe evaluation also for SCD.De facto the recent kidney donor risk index (KDRI) automatically offers the evaluation for any kidney.

The aim of this review is to describe the aforementioned evaluation criteria of ECD kidneys and to describe how they have changed with time.

SELECTION CRITERIA OF THE ARTICLES INCLUDED IN THIS REVIEW AND THEIR DRAWBACKS

The criteria to evaluate the kidneys have been histological,clinical and mixed histological-clinical.We have searched for all the papers concerning these points.The main studies concerning the most important scoring systems are shown on Table 1.With the exception of the two single centre studies as Maryland Aggregate Pathology Index (MAPI) and the Irish nomogram,all the studies considered included a large number of patients with the limitation to be retrospective in the attempt to validate the original findings.Clearly,in this review are also included articles documenting the drawbacks of the different scoring systems and these articles may include a limited number of patients.Similarly,the studies evaluating the omics on the renal biopsies or on the donor urine have a limited number of patients.

HISTOLOGICAL EVALUATION OF DONOR KIDNEYS

Figure1 Main donor,procurement and graft related factors influencing the post-transplant outcomes.

By 1999,Karpinskiet al[5]considering that kidneys from high risk donors had worse outcomes in the recipient after transplantation tried to establish which donor or kidney variables were most relevant to these poor outcomes.For high donor risk,they considered donation after cardiac death donors,donors over 55 years of age,donors with a history of hypertension or diabetes,and donors with abnormal kidney anatomy or abnormal renal function[6].The study found that a low calculated creatinine clearance (CrCl) and donor kidney pathology were the main predictors of worse outcomes

In particular,the donor renal pathology was scored 0-3 in each of four distinct aspects：Glomerulosclerosis,interstitial fibrosis,tubular atrophy and vascular disease(Table 2).Previous studies have documented the relevance of pre-implantation histological findings on recipient outcomes[7-9].None of these studies had been concordant,and the study of Karpinskiet al[5]may be considered a pioneering study documenting the relevance of the pathology score over the transplant outcomes.

Since the study of Karpinskiet al[5],several studies have documented the relevance of the pathology score of donor kidneys over the outcomes,while other studies did not find a similar usefulness of the pathology score.

One of the most important studies in favor of the pathology score has been the study of Remuzziet al[10].According to this study,the pathology score allows transplant kidneys with a score up to 3 to be used as single kidneys,while kidneys with a score from 4 to 6 are better allocated as dual transplants and kidneys with a score of 7 or higher should be discarded.

Additionally,the study documents the importance of the pre-transplant renal biopsy for donors over 60 years when comparing the renal outcomes with and without biopsy (Figure 2).

In a different study,Mancillaet al[11]suggested the utility of zero-time biopsy in the case of living donor kidneys,particularly for donors with borderline renal function or with a history of familial renal disorders[12,13].In a study from Kayleret al[14]a correlation of histological findings on pre-implantation biopsy with kidney graft survival was also found but was restricted to vascular lesions,while glomerulosclerosis and low-grade interstitial fibrosis did not have statistical significance.

Based on 371 pre-transplant biopsies and correlating the findings with posttransplant outcomes,Munivenkatappaet al[15]developed the MAPI.In the study,glomerulosclerosis,glomerular size and periglomerular fibrosis in addition to vascular pathology and arteriolar hyalinosis were considered in developing the MAPI score (Table 3).The authors found that the five-year actuarial graft survival rate was related to the MAPI scoring (Figure 3) and that the MAPI score at the multivariate analysis correlated with the risk of graft failure better than any other clinical parameter (Table 4).This study suddenly received several comments,which brought up several unanswered questions about the relevance of pre-transplant biopsies in predicting post-transplant outcomes.Many of these questions were raised by Nickeleit[16].

One point that is not clarified is whether wedge specimens or needle biopsies should be used.This issue is well described in a further paper[17]that considers wedge biopsies to be safer and superior to core biopsies in finding significant findings.

Another point is whether frozen or paraffinized sections should be used,even if the original MAPI score found paraffinized sections to be more reliable.

Table1 Descriptive table of selected clinical scoring system

ECD：Expanded criteria donor;KDRI：Kidney donor risk index;OPTN：The Organ Procurement and Transplantation Network;SCD：Standard criteria donor.

Additionally,it should be better defined when zero-time biopsies should be taken：before or after reperfusion.Biopsy time is relevant in detecting the complement activation that is predictive of early antibody mediated rejection[18].

An important point,not well considered by the MAPI score is how the lesions should be scored and whether the Banff criterion is appropriate[19].This point is relevant for comparing zero-time biopsies with subsequent post-transplant biopsies.Nickeleit[16]’s conclusions were that much remains to be determined about zero-time biopsies and that consensus guidelines remain to be defined.

Recommendations on these points have been given by two German workshops and described by Pisarskiet al[20]in 2016.The German recommendations advocate a detailed assessment of the findings and do not agree with the recommendations of the Interpretation Biopsy Banff Working Group[21],whose approach is adopted for a general pathologist,without specific training in the field.

The issue of an expert pathologist was addressed in 2012 in a study of the preimplantation biopsies in the Organ Procurement Organization (OPOS) that found a lack of concordance among OPOS pathologists[22].The lack of a correlation between the findings of on-call pathologists and the lack of association between their findings and the transplant outcomes is highlighted by two papers[23,24]that advocate for specific training in renal pathology to optimize the histological evaluation of donor kidneys.It could also be argued that a renal pathologist “per se” could not be expert enough in evaluating such biopsies.Probably a specific training should be the best solution.

By 2011,Muelleret al[25],reviewing several studies on histopathology-based variables at zero-time biopsies,highlighted the limitations due to sampling errors,confounding clinical variables,and inter-observer variability[26,27]and advocated for a validated approach for the analysis of pathology findings.In particular,they advocate for the use of omic technologies such as proteomics,transcriptomics and metabolomics that could have the potential to improve the significance of the histological findings.Table 5 highlights the principal studies that were conducted until 2011[28,39].

A study from Krolet al[40],documented that the apoptosis of tubular epithelial cells in pre-implantation biopsies is related to DGF.Their findings were confirmed by another study[41]that found a relationship between highBAX/BCL2expression in preimplantation biopsies and DGF,confirming that apoptosis-related gene expression levels are predictors of DGF.

A recent study[42]confirmed that zero-time biopsies in ECDs showed a significant increase in the transcripts ofMCP-1,RANTES,TGF betaandIL 10,documenting a higher gene expression of inflammatory cytokines in ECDs that could predict the post-transplant outcome.

In recent years,several studies,often retrospective,and several reviews and metaanalyses did not confirm the utility of zero-time biopsy in allocating or discarding ECD kidneys.Wanget al[43]reviewed 47 studies published between 1994 and 2014,where each study included pre-transplant biopsies format least 50 donors and compared the histological findings with post-transplant outcomes.Overall,15 scoring systems were proposed by the studies,but none were able to correlate with posttransplant outcomes.

Table2 Histological score according Karpinski

Naesens[44]reviewed the problems and the utility of zero-time biopsy and highlighted that the major problems were the wedgevscore needle biopsy[45,46];frozenvsparaffin-embedded tissue[47,48];pathologist’s experience[23,24];different composite histological scoring such as the Pirani score[49],Chronic Allograft Damage Index(CADI)[50],and Donor Score[23];and the lack of utilizing hard clinical end-points in evaluating graft and recipient outcomes.The author concluded that zero-time biopsies are not useful for assigning or discarding kidneys or improving dual kidney transplantation programs.The author recognizes that the molecular phenotype in pretransplant biopsies could be useful in donor selection and in peri-transplant management even if the time required could make such a procedure difficult[51-54].

Two recent Italian studies on the utility of pre-implantation biopsy in allocating ECD kidneys[4,55]concluded that histological evaluation was not superior to donor clinical evaluation in allocating ECD kidneys either as a single kidney or as a dual kidney transplant.The authors concluded that,according to their experience,the histological score poorly evaluates the donor kidney quality.Accordingly,the use of histological criteria to assign as single or dual kidneys does not seem to offer advantages over the evaluation made on clinical basis.

A Banff Pre-implantation Biopsy Working Group has been established to develop guidelines for the interpretation of pre-implantation renal biopsies[56].The last working group meeting stated that to date,histological parameters are poorly correlated with post-transplant outcomes and that remain significant limitations in understanding the role of pre-implantation biopsies.

Recently,Carpenteret al[57]from Columbia University examined their experience and compared procurement biopsies with reperfusion paraffin-embedded biopsies and with post-transplant biopsies.All the findings were then correlated with allograft failures and patient deaths.No agreement has been found between frozen procurement biopsies and paraffin-embedded biopsies,and frozen procurement biopsies were poorly correlated with post-transplant biopsies and the hard end-point considered.

COMBINED CLINICAL AND HISTOLOGICAL EVALUATION OF DONOR KIDNEYS

A different approach to evaluating ECD kidneys has been to combine histological findings with clinical donor-related parameters.The latter have been identified since the publication of the study by Portet al[58].In a study in 2001,Verranet al[59]found that the combination of abnormal biopsy findings with donor age and donor cardiovascular disease and hypertension was associated with poor outcomes.

Figure2 36 month graft survival for donors over 60 years according pre-transplant biopsy.

In an Italian study[60],donor kidneys were assigned with good results according to donor renal function [estimated glomerular filtration rate (eGFR) under or over 50 mL/min] and the previously mentioned Karpinski score.

The largest study that evaluated the predictive value of clinical and histological findings taken together was conducted by Anglicheauet al[61].The authors,evaluating 313 kidney transplants from donors aged ＞50 years,developed the so-called Anglicheau score.The best predictive parameters were a history of hypertension in the donor,serum creatinine levels under or over 1.5 mg/dL and glomerulosclerosis less than or over 10%.These parameters in the multivariate analysis significantly correlated with renal function at 1 year post-transplantation.

A different study[62]recognizes the utility of zero-time biopsy,but,as none of the histological variables and scores provided a good prediction of post-transplant outcomes,the histological findings need to be integrated with all the known donorrelated clinical parameters.

Finally,a very recent Spanish study[63]highlights the utility of evaluating the pretransplant donor biopsies in the donor with the highest kidney donor profile index(KDPI) that is based on several deceased donor variables.

CLINICAL EVALUATION OF DONOR KIDNEYS

In an attempt to improve the evaluation of the donor kidneys,principally in the US,where the donor kidney evaluation is strictly connected with their discard or their allocation to different recipients according to national programs,several clinical donor quality scoring systems have been performed.

The first one was the characterization and a better definition of ECDs.According to the report of the Kidney Working Group[1],kidneys belonging to the ECD were kidneys with a relative risk of graft failure of 1.7 with respect to standard kidneys.These kidneys are characterized by a donor age older than 59 years with two of the following characteristics：cerebrovascular accident as cause of death,history of hypertension or creatinine over 1.5 mg/dL[2].

Nyberget al[64]evaluated 241 consecutive cadaveric renal transplants and gave a score based on recognized clinical factors responsible for DGF.These factors were age,cause of death,history of hypertension,diabetes mellitus,creatinine clearance and presence in the donor of renal artery stenosis.A scoring system was developed from these seven donor variables,allowing stratification of cadaver kidneys into four classes (grades A,B,C,D).Univariate and multivariate analyses were performed,and a significant decline in early renal function was observed with an increase in the score.Additionally,the multivariate analysis had a better prognostic value with respect to each single variable considered in the univariate analysis.

Later,Nyberget al[65],in an attempt to validate his scoring system,applied the analysis to a wider population,including 34324 transplant patients from the UNOS registry in the period between 1994 and 1999.This study allowed us to evaluate the feasibility of the score on a larger follow-up.The study allowed the recognition of five clinical variables as predictive of a poorer outcome [age,cause of death,history of hypertension,creatinine clearance and human leukocyte antigen (HLA) mismatch].This score was called the Deceased Donor Score or Nyberg score and was able to predict renal function at 12 mo and graft survival at 6 years (Figure 4).

A further study by the same author[66]also confirmed these data for kidneysreceiving machine reperfusion.

Table3 Maryland Aggregate Pathology Index scoring system for pre-transplant kidney biopsies

To further improve clinical factors able to evaluate kidney status and to predict outcomes after transplantation,Scholdet al[67]studied different clinical variables that were applied to transplants included in the National Scientific Transplant Registry from 1996 to 2002.

The variables were age,race,and history of hypertension,diabetes mellitus,and cause of death,cold ischemia time,HLA mismatch,and immunological status and CMV status.This was called the Donor Risk Score and allowed for the calculation of the multivariate estimates for graft loss by donor grade (Figure 5).

A further study[68]compared the different clinical risk scores and documented that the Donor Risk Score was better associated with subsequent allograft function.

ECD-KDRI-KDPI

As already mentioned,by 2002,in an attempt to improve the utilization of marginal deceased donor kidneys,the concept of ECDvsSCD was introduced[1,2].With time this dichotomy (SCD/ECD) demonstrated several drawbacks.Indeed,the experience documented that several kidneys labeled as ECD performed well,while other kidneys labeled as SCD did not perform well[69].To improve these limitations other different scoring systems have been attempted.The donor score of Nyberg and the donor risk score of Schold have been described.Additionally,Irishet al[70]applied a nomogram aimed at predicting the risk of DGF based on 16 donor and recipient risk factors.Mooreet al[68]documented that Schold’s donor risk score is the scoring system that best predicts graft outcomes,but the need still remains for a simple and validated system that applies to the entire donor population viewed as a continuum and not in a dichotomous fashion.

In 2009,Raoet al[71]analyzed 69440 deceased donor adult transplants registered in the Scientific Registry of Transplant Recipients (SRTR) and proposed a new continuous KDRI for deceased donor kidneys combining donor and transplant variables.

Rao’s KDRI included 14 donor and transplant factors,each associated with shorter graft survival.Table 6 shows the mentioned risk factors.

The KDRI is a continuous spectrum for any kind of donor (ECD and SCD) and allows for dividing the donor population into quintiles based on their KDRI.By the end of 2014,the KDRI was implemented by the OPTN[72].Indeed,as some transplant factors are not known at the time of transplant,the donor-only KDRI based on 10 donor factors has been implemented.

All the mentioned donor scoring systems are shown in Table 1[73].Woodsideet al[74]examined the SRTR data from 2002 to 2010,and applying the KDRI,they found that kidneys belonging to the same KDRI quintile had similar outcomes independently of their belonging to ECD or SCD.However,ECD kidneys had a higher discard rate.

The use of the KDRI was further validated by several studies.Junet al[75]examined the use of the KDRI in donors with acute kidney injury (AKI) and found a good correlation between KDRI quintiles and graft outcomes.

A different study[76]documented that the KDRI was a good prognostic tool for graft outcomes in deceased donor kidney transplantation with a short cold ischemia time.In this study,the KDRI correlated with renal function at 1 year,and a high KDRI was associated with a high risk of graft failure.

Figure3 Five years graft survival for the study population according low,intermediate and high Maryland Aggregate Pathology Index score ranges.

Recently,a Spanish study validated the usefulness of the KDRI in a European population[77].The study evaluated 144 renal transplants.All kidneys transplanted were evaluated by the KDRI and biopsied.The aims of the study were to verify the concordance between the KDRI and the histological findings and to validate the prognostic value of the KDRI for transplant outcomes.The study concluded that there was a poor concordance between the KDRI and histological score and that the KDRI had a good prognostic value.

Strictly connected with the KDRI is the KDPI.The KDPI represents the relative risk of graft failure in the case of a particular deceased donor compared to a reference donor.The KDPI was introduced in 2014 in the US[78]and is derived by ranking the KDRI on a scale of 0-100% with reference to a donor cohort in the OPTN.It is useful and is represented by a number that helps in deciding the allocation of a specific organ[79].The KDRI and KDPI are strictly related.

These scoring systems have advantages over the ECD system because they represent a continuum,are based on 10 donor factors and represent a measure of donor quality.

Limitations of the KDRI and KDPI are represented by the fact that they do not include all of the donors’ factors that could impact the graft outcome.Additionally,the KDPI is a measure of the donor and is not specific for each kidney taken individually.

The KDPI is useful for introducing the concept of the so-called longevity matching.The concept consists of allocating kidneys with a higher KDPI to patients on dialysis with a lower life expectancy.A retrospective study[80]documented those patients older than 50 years or with a long waiting list time who were transplanted with kidneys with a high KDPI had a better survival than similar patients remaining on dialysis.This is particularly evident for patients older than 70 years[81].Notwithstanding,a German study[82]reporting the experience of transplanting kidneys with a high KDPI observed that poor kidney quality,even when matching donors and recipients is the main factor responsible for poor outcomes.Several studies have evaluated the utility of the KDPI even outside of the US.

In a retrospective study,Lehneret al[83]evaluated the utility of the KDPI in almost 1000 European kidney transplants.The study found rather good outcomes in the case of donors with a very high KDPI.A Spanish study[84]evaluated the KDPI score on 389 transplants.The study documented that only the KDPI correlated with the risk of graft failure.This study also documented the utility of the KDPI measure in a cohort of European patients.

To further improve the KDPI,a retrospective study[85]was conducted in the US.The study evaluated the KDPI in adult transplant recipients in the OPTN/UNOS database from 2000 to 2015.This study,while validating the usefulness of the KDPI,found that terminal serum creatinine of the donor (one of the components of the KDPI) is not a useful variable.

Another European study[86]analyzed 1,305 kidney transplants.The study retrospectively applied the KDPI in 889 deceased donors and the living donor kidney profile index (LKDPI) in 416 living donors using the LKDPI realized by a US study for living donation[87].The European study was able to validate both the KDPI and LDKPI.

A major concern is what to do with donor kidneys with very a high KDPI (＞80%).

In the US,the discard rate of these kidneys is approximately 50%.However,the allocation of kidneys with a KDPI higher than 80% in patients older than 60 years results in a lower patient mortality compared to patients who remain on the waiting list[88].Indeed,several kidneys with a KDPI higher than 80% are viable.A recentstudy[89]evaluated the 1-year eGFR and graft failure for kidneys transplanted with a KDPI higher than 80%.The discard of such kidneys had been decided with the help of a pre-Tx kidney biopsy,renal resistance and kidney injury biomarker levels.The 1-year eGFR was low but satisfying.The authors request the use of new biological tools for a proper evaluation of these kidneys.

Table4 Cox Multivariate analysis showing association of Maryland Aggregate Pathology Index score and clinical parameters to risk of graft failure

An Italian multicenter study tried to reduce the discard rate of kidneys with a KDPI higher than 80% using pre-transplant kidney biopsy for these kidneys[90].The discard rate was reduced from 50% to 15%-37% according to the KDPI.The 1-year eGFR was lower for these marginal kidneys,but the graft survival was similar to that of standard kidneys.The study highlighted the utility of pre-transplant biopsy for kidneys with a very high KDPI.

Finally,a recently raised relevant question is whether the KDPI may be universally applied in allocating marginal kidneys or whether it is UNOS specific.A recent study from Ruggenentiet al[91]documented the allocation and good graft survival of 37 renal transplants with donors with a KDPI between 96% and 100% after a pre-transplant biopsy.These kidneys should have been discarded according to the UNOS criteria[92].Similar findings have come from a previous study by Ekseret al[93].The 5-year graft survival was 91%,and the mean KDPI was 97%.More than 80% of these kidneys should have been discarded according to the UNOS[94].

The question of UNOS specificity of the KDPI is examined in a recent study by Ruggenentiet al[95].According to the author,the difference in ethnicity may only partially explain the different results and the different discard rates of UNOS and several European studies[96].The author highlights the usefulness of pre-transplant biopsy for kidneys of donors with a very high KDPI.

In conclusion,the KDRI/KDPI represents an easy scoring system that could facilitate the decision to discard organs or allocate them in the best way.

According to several studies,the KDPI may also be applicable to European patients,even though this point is to date debated.

Based on the KDPI,the UNOS is implementing new allocation systems such as“l(fā)ongevity matching”.Each candidate willing to participate in the “l(fā)ongevity matching” will receive an “estimated post-transplant survival score” (EPTS) and will receive a graft according to the matching KDPI/EPTS.

The allocation of kidneys with the highest KDPI is debated.Often,these kidneys are discarded[97],but the use of pre-transplant biopsy may allow allocation of many of these kidneys,thus reducing the discard rate[98].

MACHINE PERFUSION AND PERFUSATE BIOMARKERS

Hypothermic machine perfusion is increasingly used in deceased donor kidney transplantation,but the question still remains on how efficient are MP in assessing the quality of an organ?

One study evaluating the reasons for discarding 12536 ECD kidneys found that 15% of perfused kidneys were discarded partly based on high renovascular resistance(RR)[99].In a prospective study by Jochmanset al[100]RR values of 302 MP kidneys were evaluated.The study conclusions were that RR as a standalone quality assessment tool cannot be used to predict the graft outcomes.

More recently,Parikhet al[101]in a prospective observational cohort study examined the association between pump parameters and graft outcomes.They found an association between 1 h perfusate flow and DGF but with a border line value.

In conclusion,according the currently available data,there is a weak correlationbetween perfusion parameters and graft outcomes and additional studies are needed.

Table5 Studies on molecular markers measured in 0-h biopsies (up to 2011)

FUTURE PERSPECTIVES AND EMERGING TECHNOLOGIES

All the scoring systems,either histological or clinical,need to be improved with the help of new tools.Indeed,several cited studies advocate for newest approach in the evaluation of donor kidneys.Nickeleit[16]stated that new consensus guidelines remain to be defined on zero-time biopsies.Muelleret al[25]highlighting the confounding variables,advocate for the use of omic technologies in the evaluation of kidney biopsies.This point is also highlighted by the Banff Pre-Implantation Biopsy Working Group[56].The usefulness of biomarkers in the evaluation of donor kidneys has also been highlighted by another recent study[90].

Figure4 Grade of deceased donor kidney score significantly influenced graft survival at 6 years after transplantation.

There are a number of emerging technologies to examine an organ at molecular level ranging from proteomics to metabolomics to transcription studies.

The most important study on proteomics is the study of Reeseet al[102]who examined the association between four different biomarkers and the post-transplant renal function.All the urine injury biomarkers strongly associated with donor AKI,but resulted of limited value in predicting DGF or early graft function

By using transcription analysis,Scianet al[103]validated a set of three genes (CCL5,CXCR4andITGB2) that was up regulated in kidneys with a low eGFR posttransplantation.

Gustafsonet al[104]still by transcription analysis found a set of 13 genes (Table 7)associated with allograft loss at two or three years after transplantation.

By metabolomics studies,Guyet al[105]found in the perfusate of the hypothermic machine significant lower levels of gluconate,glucose,inosine and leucine in kidneys with DGF.

Finally,a novel technique able to recondition the kidney and to restore normal function prior to transplantation is theex vivonormothermic perfusion.Phase I studies in ECD documented its safety and feasibility in clinical practice[106].

Some studies are ongoing,but their results are to date unknown.

An important study aims to evaluate the relevance of molecular biomarkers of aging in the blood of donors.This study (Senesce Test) has been completed,but no results are available yet (NCT02335333)[107].Another NIH study coordinated by Yale University is testing biomarkers characteristic of renal injury in the urine of the donor and in the perfusion media (NCT01848249)[108].

The PREDICTION study aims to evaluate the improvement in viability of marginal kidneys treated by pulsatile perfusion[109].

CONCLUSION

The increase in the demand of kidneys for transplantation may only be satisfied with the increase in the use of marginal donors as kidneys from aged donors or with the use of donation after cardiac death donors.

Such kidneys need to be carefully evaluated either to be discarded or for a fair allocation.

The histological evaluation met several drawbacks as the time of the biopsy (pre or post reperfusion,the type of biopsy (wedge versus core biopsy),the pathologist involved in the evaluation (pathologist on-call or trained pathologist in this field).

Additionally,the difficulty of obtaining adequate histological analysis from pre implantation biopsies and the risk/benefit considerations to prolong cold ischemia time waiting for chronic histological abnormalities that often show poor correlation with clinical outcomes represents the most relevant drawback.All these drawbacks led to give more importance to the clinical evaluation of the donor.The KDRI/KDPI is an easily applicable scoring system,but this system also has its drawbacks especially in the evaluation of donors with the highest KDPI.

In the US,the use of KDPI led to a very high discard rate of the marginal donor kidneys,while other studies documented that several of these kidneys might be usefully transplanted.

Figure5 Multivariate estimates for graft loss by donor grade (Hazard ratio expressed as mean +/- confidence interval.

Overall,is not easy to establish how many centers have taken part to the different scoring system as many of them are retrospective studies.

The elaboration of the Port scoring of standard criteria donors versus expanded criteria donors has been done comparing retrospectively 24756 SCD versus 4312 ECD from almost all the UNOS centers.

The MAPI has been done in a single center considering 371 transplants.

The Nyberg deceased donor score was made in three steps.In a first step 241 transplants were enrolled in two centers.Then in the attempt to give more strength to the scoring system,this was evaluated retrospectively on 34324 UNOS kidney transplants and in a third phase on 48952 UNOS kidney transplants.

The Donor risk score of Schold was evaluated retrospectively on 45850 data from SRTR.

The DGF nomogram of Irish was evaluated in a single center in UK on 217 prospective transplant patients.

Finally the KDRI of Rao was retrospectively evaluated on 69440 patients from SRTR.Subsequently the scoring was evaluated prospectively in different countries.

A hope for the future seems to come from the use of biomarkers.However,to date the use of urine biomarkers offers discordant results and does not provide sufficient power to be used in the kidney evaluation.

According recent studies,the use of pre-implantation biopsy has been shown to have its major utility in the evaluation of kidneys with a very high KDPI.

A very recent study from Moeckliet al[110]helps in clarifying what’s new in the current and emerging techniques of kidney evaluation.In particular the study concerns the use of omics and states that the most promising is transcriptome profile,also according the already cited studies.

Waiting for the advent of omics it seems that the best strategy in evaluating kidneys for transplantation is the clinical one.In the case of a very high KDRI pretransplant biopsy may be useful in allocating or not the kidneys

Table7 Genes included in the study