劉　芳　 趙　鑫　 潘玉瑾　 郭惠宣　 林　琦　 胡筱敏

(東北大學(xué)資源與土木工程學(xué)院, 沈陽(yáng) 110819)

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高效異養(yǎng)硝化細(xì)菌富集與強(qiáng)化脫氮

劉芳 趙鑫 潘玉瑾 郭惠宣 林琦 胡筱敏

(東北大學(xué)資源與土木工程學(xué)院, 沈陽(yáng) 110819)

高效異養(yǎng)硝化菌;高通量測(cè)序;生物強(qiáng)化;脫氮;SBR

自然水體的氨氮污染,是引發(fā)富營(yíng)養(yǎng)化和導(dǎo)致水生生態(tài)系統(tǒng)破壞的重要元兇.水體富營(yíng)養(yǎng)化會(huì)引起某些藻類的惡性繁殖,大量藻類的死亡會(huì)耗去水中的氧氣,引起魚(yú)蝦類水產(chǎn)物的大量死亡,致使湖泊退化甚至消亡.據(jù)統(tǒng)計(jì),我國(guó)平均每年有20個(gè)天然湖泊消亡[1].

1　實(shí)驗(yàn)材料與方法

1.1實(shí)驗(yàn)裝置

高效異養(yǎng)硝化微生物的富集和強(qiáng)化脫氮實(shí)驗(yàn)均在SBR中進(jìn)行.SBR有效容積為5 L,時(shí)控開(kāi)關(guān)控制進(jìn)水、曝氣和出水時(shí)間,氣體流量計(jì)控制曝氣量.整個(gè)實(shí)驗(yàn)過(guò)程中SBR反應(yīng)周期均為進(jìn)水0.25 h, 曝氣6 h,靜置0.5 h,出水0.5 h,停滯0.75 h;水力停留時(shí)間HRT為8 h.SBR實(shí)驗(yàn)裝置系統(tǒng)如圖1所示.

1—進(jìn)水箱;2—進(jìn)水泵;3—空氣轉(zhuǎn)子流量計(jì);4—微孔曝氣頭;5—SBR反應(yīng)器;6—空氣泵;7—液體轉(zhuǎn)子流量計(jì)圖1　SBR實(shí)驗(yàn)裝置系統(tǒng)

1.2實(shí)驗(yàn)方法

1.3測(cè)定方法

微生物群落結(jié)構(gòu)分析委托上海美吉生物醫(yī)藥科技有限公司完成,實(shí)驗(yàn)流程為基因組DNA提取→設(shè)計(jì)并合成引物接頭→PCR擴(kuò)增和產(chǎn)物純化→PCR產(chǎn)物定量和均一化→MiSeq PE文庫(kù)制備→MiSeq高通量測(cè)序.

2　結(jié)果與討論

2.1異養(yǎng)硝化菌群的富集

(a) 去除率

(b) 質(zhì)量濃度

圖3　富集前后微生物群落結(jié)構(gòu)圖

2.2異養(yǎng)硝化菌群的生物強(qiáng)化

圖4　出水COD和NH+4-N質(zhì)量濃度

圖5　三氮濃度隨時(shí)間變化

圖6　進(jìn)、出水COD和NH+4-N濃度

3　結(jié)論

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Enrichment and enhanced nitrogen removal of heterotrophic nitrifying bacteria

Liu Fang Zhao Xin Pan Yujin Guo Huixuan Lin Qi Hu Xiaomin

(School of Resources and Civil Engineering, Northeastern University, Shenyang 110819, China)

heterotrophic nitrifying bacteria; high-throughput sequencing; bioaugmentation; nitrogen removal; sequencing batch reactor

10.3969/j.issn.1001-0505.2016.04.022

2015-12-01.作者簡(jiǎn)介: 劉芳(1988—)，女，博士生；胡筱敏(聯(lián)系人)，男，教授，博士生導(dǎo)師，hxmin_jj@163.com.

國(guó)家水體污染控制與治理重大專項(xiàng)資助項(xiàng)目(2013ZX07202-010).

10.3969/j.issn.1001-0505.2016.04.022.

X52

A

1001-0505(2016)04-0807-05

引用本文: 劉芳,趙鑫,潘玉瑾，等.高效異養(yǎng)硝化細(xì)菌富集與強(qiáng)化脫氮[J].東南大學(xué)學(xué)報(bào)(自然科學(xué)版),2016,46(4):807-811.