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Fig. 1 The 4th generation HASMCs (Inverted microscope ×400)

Fig . 2 Immunohistochemistry for α-SM-actin of the 4th generation HASMCs (Immunohistochemistry ×400)

Fig. 3 HASMCs expressed TLR4 of each group (Immunocytochemistry ×200)A：Normal control group expressed less TLR 4；B：Asthma group expressed less TLR 4；C：TNF-α group expressed more TLR 4；D：TLR4 antibody group express less TLR 4；HASMCs：Human airway smooth muscle cells；TLR4：Toll like receptor 4；TNF-α：Tumor necrosis factor-α