亚洲免费av电影一区二区三区,日韩爱爱视频,51精品视频一区二区三区,91视频爱爱,日韩欧美在线播放视频,中文字幕少妇AV,亚洲电影中文字幕,久久久久亚洲av成人网址,久久综合视频网站,国产在线不卡免费播放

        ?

        Optimization of Extraction Program of Sea Buckthorn Seeds Proanthocyanidins by Combining Uniform Design and Microsoft Excel

        2012-10-28 08:17:00TIANYaxinHANXiaoyunWANGYunlaiKANGChuanhong
        食品科學(xué) 2012年16期
        關(guān)鍵詞:優(yōu)化實(shí)驗(yàn)方法

        TIAN Ya-xin,HAN Xiao-yun,WANG Yun-lai,KANG Chuan-hong*

        (1. College of Life Science, Heilongjiang University, Harbin 150080, China;

        2. College of Chemistry and Materials Science, Heilongjiang University, Harbin 150080, China)

        Optimization of Extraction Program of Sea Buckthorn Seeds Proanthocyanidins by Combining Uniform Design and Microsoft Excel

        TIAN Ya-xin1,HAN Xiao-yun1,WANG Yun-lai1,KANG Chuan-hong2,*

        (1. College of Life Science, Heilongjiang University, Harbin 150080, China;

        2. College of Chemistry and Materials Science, Heilongjiang University, Harbin 150080, China)

        A novel optimization method based on uniform design and function LINEST of Microsoft Excel was proposed to explore the extraction of proanthocyanidins from sea buckthorn seeds. The proposed multiple regression equation was calculated with the function LINEST. This method was not only a flexible method, but also could avoid high expenditure and fixed algorithm while using other professional software for data processing. The validation experiments showed that the extraction rate of proanthocyanidins from sea buckthorn seeds revealed an increase by 16.43%. This method is easy to extend and may hold promise for other scientific applications.

        optimization;uniform design;LINEST;regression equation;proanthocyanidins

        For a multi-factor, multi-level experiment, there were several statistical experiment design methods, for example factorial design, response surface design, orthogonal design and uniform design and so on. The uniform design (UD) was one of space filling designs and it sought experimental points to be uniformly scattered on the domain[1-2]. The interrelationship among the factors could be uncovered by the UD in a few experimental runs. In recent years, the UD was used more and more widely in many fields because of its cost-efficiency, robustness and flexibility[2-3], whereas the treatment of the UD experimental results had always been a problem. There was much data processing software for the UD, but they were very difficult to adapt to different actual experiments, and the results calculated by using their fixed algorithms may not be satisfied.

        The Excel spreadsheet function “LINEST” was a complete least square curve fitting routine that produces uncertainty estimates for the fit values[4]. Considering that the least square was an ideal method on the calculation of regression equation at present, the LINEST could be used to deal with the experimental results of the UD. The calculation of the multiple regression equation by the LINEST was the emphasis in this paper.

        Sea buckthorn (Hippophae rhamnoides L.) was rich invitamins, flavonoids, polyphenols, organic acids and other nutritional and bioactive compounds[5-7], and had much physiological and pharmacological activities, for example antioxidation properties[8], antibacterial activities[9], protective effects on cardiovascular system[10], anti-inflammatory actions[11]and so on. People overmuch paid attention to research into sea buckthorn flavonoids and oil but ignored a group of vital compositions—proanthocyanidins. Those were polyhydric phenols compounds[12]which had application value greater than others. So far, the research on sea buckthorn proanthocyanidins had just begun. Taking industrial production costs and requirements into consideration, the ethanol extraction method was used to study extraction process of sea buckthorn proanthocyanidins by some researchers. The extraction yield was considered only by every single factor test by them, without considered by combinable several factors simultaneously. At present, the UD was one of the best methods of multi-factor combination analysis. In this paper extraction program of sea buckthorn seeds proanthocyanidins was designed by the UD, and then multiple regression equation was calculated by the LINEST. After validated, the extraction yield of sea buckthorn seeds proanthocyanidins was up to 14.46%, increased by 16.43%.

        1 Materials and Methods

        1.1 Materials, reagents and instruments

        The sea buckthorn seeds were from Sunwu County Forestry Administration, Heilongjiang Province, China. The standard samples of grape seeds proanthocyanidins (purity was above 99.5%) was from Jianfeng Natural Products Research and Development Co. Ltd. Tianjin City, China. Petroleum ether, ethanol, n-butanol, hydrochloric acid and methanol were all analytical-reagent grade.

        Tissue triturator was from Suzhou Ou-Bei Scientific Instrument Co. Ltd., China. Rotary evaporator was from Suzhou Da-long Instrument Co. Ltd., China. TU1800 UV-VIS Spectrophotometer was from Beijing Purkinje General Instrument Co. Ltd. China. pH meter was from Shanghai Jing-ke Industry Co. Ltd., China.

        1.2 Extraction processes

        A certain amount of freeze-dried sea buckthorn seeds were shattered by using tissue triturator, and filtrated by 80-mesh filter. The seed powder was put into circumfluent equipment in which there was an amount of petroleum ether. After degreasing for definite time at temperature of 30 ℃, and then removing petroleum ether and evaporating it, defatted powder of sea buckthorn seeds was gained. 10 g of defatted powder of sea buckthorn seeds was weighed up, and then the proanthocyandins were extracted on condition that definite volume of aqueous ethanol, pH value, water bath temperature and time. After extracted it three times, combined extract were concentrated on a rotatory evaporator. In this way, the raw proanthocyanidins extract was gain.

        1.3 Determination of contents of proanthocyanidins

        By calculating standard curve function, the content of proanthocyanidins was determined by butanol/HCl assay[13-14]. 0.010 g of grape seed proanthocyanidins standard was weigh up accurately, and then dissolved in methanol, the volume to 20 mL. Respectively, 0, 0.2, 0.4, 0.6, 0.8 mL and 1.0 mL of solution above was suctioned accurately into 25 mL cuvette, in which 6 mL of n-butanol/HCl (V/V is 95:5) was added, and then 70% methanol was used to fill up to 7 mL. The mixture was heated in a boiling water bath for 40 min, placed in cold water to cool 15 min. The absorptance value was measured at 546 nm wavelength by reagent blank as reference. The standard curve function was calculated by function LINEST of Microsoft Excel software.

        According to process of calculating standard curve function, the relationship of concentration of grape seed proanthocyanidins standard and UV absorbance value (Table 1) was determined. The standard curve function y=14.1799x +0.0069 was gained by function LINEST of Excel and R2of the function was 0.9994. F statistics value was 7111, and F-critical value was 22 while confidence level was 0.01 by using the function FINV of Excel.

        Table 1 Relationship between the content of proanthocyanidins from sea buckthorn seeds and UV absorbance

        0.050 g of extract products was weigh up accurately, and then dissolved in methanol, the volume to 20 mL. 1 mL of solution was suctioned accurately into 25 mL cuvette, subsequent experimental procedures as above. The content of proanthocyanidins of extract products was calculated by standard curve function gainded above.

        After gained the content of proanthocyanidins of extraction products, the extraction yield could be calculated.

        2 Results and Analysis

        2.1 Uniform design

        This experimental program was designed by using the UD table U11(1110)[3,15](Table 2, Table3). Degreasing time (DT), ethanol concentration (EC), the ratio of ethanol to materials (E/ S), pH value (pH), extraction temperature (ETE) and extraction time (ETI) were selected as optimization factors. The extraction yield (EY) of proanthocyanidins was 12.42%, as selected 10 hours of DT, 70% of EC, 7:1 of E/S, 6 of pH, 40 ℃ of ETE and 120 minutes of ETI before optimizing. According to the result of single-factor experiments, the experimental program was designed and result was showed in Table 4, in which each experimental factor was fixed on the reasonable scope.

        Table 2 Uniform design table

        Table 3 Guidance for using uniform design table

        Table 4 Uniform design experimental results

        2.2 Calculation of the multiple regression equation by the Excel

        This study calculated the multiple regression equation by using the LINEST, which calculated the statistics for a line by using the least squares,, method to calculate a straight line that best fits experimental data, and then returned an array that describes the line[16-17]. The syntax of the LINEST was LINEST (known_y,s, known_x,s, const, stats). It returned the m-values which were coefficients corresponding to each x-value, the b-value which was the value of y at the point where the line crosses the y-axis and additional regression statistics. Among the additional regression statistics, value R2, the coefficient of determination, was a very important statistic. It was an indicator of how well the equation resulting from the regression analysis explained the relationship among the variables. Since the LINEST returns an array, function index of Excel could be used for returning the parameter required.

        Firstly, the quadratic equation was calculated for well reflected the impact of each factors on result. For example, the value A of the quadratic equation y=Ax2+Bx+C could be returned by using “{=INDEX(LINEST($B$2: $B$11,(A$2:A$11)^{1,2}, TRUE,TRUE),1,1)}” in Excel, and the value B, C and R2as well as. As a result, the equation y=-0.00215546x52+0.040870222x5+11.71676825(y represents EY, x5represents ETE) was got, which reflected the impact of ETE on EY (Fig.1).

        Fig.1 Calculation of R2, A, B and C for the quadratic equation y =Ax52 +Bx5+C by using LINEST of Microsoft Excel

        Secondly, for finding combinable different factors affecting the results, multiple regression equations were also calculated by using the LINEST. For example, the equation y=-0.000278456x2x4x5+12.40214326(x2represents EX, x4represents pH and x5represents ETE), calculated by using the LINEST, reflected the impact of combining EC, pH and ETE on EY (Fig.2).

        Thirdly, it was very important that to construct the much better multiple regression equation by combining the regression equation that be calculated before. The way was still using the LINEST. For example, the equations y1=-0.00215546x52+0.040870222x5+ 11.71676825 and y2=-0.000278456x2x4x5+12.40214326 were taken as known quantity, and then calculated the regression equation y= My1+Ny2+S by using the LINEST. As a result, after the parameter M, N and S of the equation was got, the new equation y=-0.001460597x52+0.027694752x5+0.000115495x2x4x5+12.202454951 was constructed (Fig.3).

        Fig. 2 Calculation of R2, E and F for the multiple regression equation y=Ex2x4x5 +F by using LINEST of Microsoft Excel

        Fig.3 Calculation of M, N and S for the multiple regression equation y=M(Ax52+Bx5+C)+N( Ex2X4X5+F)+S by using LINEST of Microsoft Excel

        It was challenge attention herein that the value R2in Fig.3 was not of the new equation but of the equation y=My1+Ny2+S. The R2of new equation could be calculated artificially by using following formulae:

        Lastly, in accordance with the above methods, different regression equations were calculated. By examining the value R2(more closer to one, more better) and F-statistic (more greater than the F-critical value(Fc), more better), whether or not to use the function was decided. The F-statistic was an even better statistical test of the goodness of fit. The F-statistic could be calculated by below formula, and Fcvalue could be calculated by function FINV of Excel.

        The formula was used in Excel, 0.01 was confidence level which could be replaced by 0.05 or other values.

        At last, the best optimized multiple regression equation was gain.

        In the above formula, x1was representative of the DT; x2was representative of the EC; x3was representative of the E/S; x4was representative of the pH; x5was representative of the ETE; x6was representative of the ETI; y was representative of the EY.

        After calculated, the R2was 0.998 and F-statistic was 225.692 of the equation. When confidence level was 0.01, the F-critical value was 27.911. It meaned the multiple regression equation calculated was the goodness of fit that R2was very close to one and F-statistic was much greater than F-critical value.

        Calculated by using Qbasic program compiled myself and multiple regression equation got above, when DT was 5 h, EC was 60%, E/S was 8, pH was 3, ETE was 20℃ and ETI was 60 min, the estimative EY 14.77% was best. Did experiment in the light of the program, the actual EY was 14.46%, which was increased by16.43% than unoptimizable program.

        3 Conclusions

        In this paper, the UD table (1110) was developed to design experiment program, and function LINEST of Excel was used to calculated multiple regression equation to optimize extraction yield of sea buckhorn seeds pranthocyanidins. After calculated and judged the values R2and F-statistic, the equation was affirmed to the goodness of fit. The whole optimized program was accepted as valid by doing experiment according to the program optimized, and the extraction yield of sea buckhorn seeds proanthocyanidins reached 14.46% which was increased by16.43% than unoptimizable program.

        If response surface design method was taken to optimize extraction program of sea buckthorn seeds proanthocyanidins by Box-Behnken design, there would be 54 runs for the experiment with 6 factors and 5 levels. It would outweigh more workload and expense than UD, and data processing would be rather complicated. For testing and verifying reliableness of the method combining UD and Excel, orthogonal design was taken by table L25(56). According to traditional data processing method that combine analysis of intuitiveness and analysis of variance (ANOVA), it was fixed on the best program that DT was 6 h, EC was 65%, E/S was 7, pH was 3, ETE was 20 ℃ and ETI was 70 min. By the best program, EY reached 14.31, which was lower than 14.46%. The reason was not shortage of orthogonal design but of data processing method. By the Excel method noted above, the regression equation and optimized result were close to the foregoing data.

        4 Discussion

        The results obtained in this study showed that the UD was a powerful tool to perform experiments with a large number of factors and a large number of levels for each factor. Simultaneously, the uniform design comprehensively takeed all of factors affected result into account, and avoided uncertainty of analyzing single factor,s effect on experimental results. As a result, the difference between the optimized estimative value and actual value was diminutive.

        However, there were a few difficulties in data processing of UD, especially calculation of multiple regression equation. A good many professional softwares of data processing, such as SPSS, SAS, were used to calculate multiple regression equation, whereas were difficulty to calculate the equation of UD. This paper gave a case study to how the LINEST was used for calculating multiple regression equation, which was an impactful and flexible method. This could be easily extended and applied to other situations.

        [1] FANG Kaitai. The uniform design: application of number-theoretic methods in experimental design[J]. Acta Math Appl Sinica, 1980, 3(4): 367-372.

        [2] FANG Kaitai. Theory, method and applications of the uniform design [J]. International Journal of Reliability, Quality & Safety Engineering, 2002, 9(4): 305-315.

        [3] LIANG Yizeng, FANG Kaitai, XU Qingsong. Uniform design and its applications in chemistry and chemical engineering[J]. Chemometr Intell Lab, 2001, 58(1): 43-57.

        [4] Microsoft. LINEST[EB/OL]. (2011-08-10). http://office.microsoft.com/ en-us/excel/HP052091551033.aspx?pid=CH062528311033

        [5] TIAN Chunjie, NAN Peng, CHEN Jiakuan, et al. Volatile composition of Chinese Hippophae rhamnoides and its chemotaxonomic implications [J]. Biochem Syst Ecol, 2004, 32(4): 431-441.

        [6] TANG Xurong, TIGERSTEDT P M A. Variation of physical and chemical characters within an elite sea buckthorn (Hippophae rhamnoides L.) breeding population[J]. Sci Hortic, 2001, 88(3): 203-214.

        [7] RANJITH A, KUMAR K S, VENUGOPALAN V V, et al. Fatty acids, tocols, and carotenoids in pulp oil of three sea buckthorn species (Hippophae rhamnoides, H.salicifolia, and H.tibetana) grown in the Indian Himalayas[J]. J Am Oil Chem Soc, 2006, 83(4): 359-364.

        [8] GAO Xiangqun, OHLANDER M, JEPPSSON N, et al. Changes in antioxidant effects and their relationship to phytonutrients in fruits of sea buckthorn (Hippophae rhamnoides L.) during maturation[J]. J Agr Food Chem, 2000, 48(5): 1485-1490.

        [9] NEGI P S, CHAUHAN A S, SADIA G A, et al. Antioxidant and antibacterial activities of various seabuckthorn (Hippophae rhamnoides L.) seed extracts[J]. Food Chem, 2005, 92(1): 119-124.

        [10] SUOMELA J P, AHOTUPA M, YANG Baoru, et al. Absorption of flavonols derived from sea buckthorn (Hippophae rhamnoides L.) and their effect on emerging risk factors for cardiovascular disease in humans [J]. J Agr Food Chem, 2006, 54(19): 7364-7369.

        [11] GANJU L, PADWAD Y, SINGH R, et al. Anti-inflammatory activity of Seabuckthorn (Hippophae rhamnoides) leaves[J]. Int Immunopharmacol, 2005, 5(12): 1675-1684.

        [12] PORTER L J, HRSTICH L N, CHAN B G. The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin[J]. Phytochemistry, 1985, 25(1): 223-230.

        [13] DALZELL S A, KERVEN G L. A rapid method for the measurement of Leucaena spp proanthocyanidins by the proanthocyanidin (butanol/ HCl) assay[J]. J Sci Food Agric, 1998, 78(3): 405-416.

        [14 ] Ministry of Health P R China. Health food testing and evaluative technical criterion[S].

        [15] FANG Kaitai. Uniform design and uniform design tables[M]. Beijing: Science Press, 1994.

        [16] KAGAN A, MARKHAM E. A better approach to designing an RTD interface with a spread sheet[J]. EDN, 2008, 15(12): 56-57.

        [17] LI Yunbo, SHEN Qiping, LI Heng. Design of spatial decision support systems for property professionals using Map Objects and Excel[J]. Automation in construction, 2004, 13(5): 565-573.

        聯(lián)合均勻設(shè)計(jì)法和Microsoft Excel優(yōu)化沙棘籽原花色素提取工藝

        田亞新1,韓曉云1,王運(yùn)來1,康傳紅2,*

        (1.黑龍江大學(xué)生命科學(xué)學(xué)院,黑龍江 哈爾濱 150080;
        2.黑龍江大學(xué)化學(xué)化工與材料學(xué)院,黑龍江 哈爾濱 150080)

        提出一個(gè)基于均勻設(shè)計(jì)方法和Microsoft Excel的LINEST函數(shù)進(jìn)行試驗(yàn)設(shè)計(jì)優(yōu)化的方法。本實(shí)驗(yàn)通過均勻設(shè)計(jì)方法設(shè)計(jì)沙棘籽原花色素提取工藝,然后通過LINEST函數(shù)計(jì)算多元回歸方程,該方法可避免因?yàn)槭褂闷渌麑I(yè)數(shù)據(jù)處理軟件的花費(fèi)和固定算法限制。驗(yàn)證實(shí)驗(yàn)表明:沙棘籽原花色素的提取率提高了16.43%,該方法可應(yīng)用到其他實(shí)驗(yàn)方面。

        優(yōu)化;均勻設(shè)計(jì);LINEST;回歸方程;原花色素

        O212.6;S788.1

        A

        1002-6630(2012)16-0091-05

        2011-09-15

        黑龍江省教育廳科學(xué)技術(shù)研究項(xiàng)目(11551376);黑龍江大學(xué)青年科學(xué)基金項(xiàng)目(QL200908)

        田亞新(1980—),男,講師,碩士,研究方向?yàn)樘烊凰幬锘瘜W(xué)。E-mail:tianyaxin@gmail.com

        *通信作者:康傳紅(1966—),女,教授,博士,研究方向?yàn)樘烊凰幬锘瘜W(xué)。E-mail:kangchh@163.com

        猜你喜歡
        優(yōu)化實(shí)驗(yàn)方法
        記一次有趣的實(shí)驗(yàn)
        超限高層建筑結(jié)構(gòu)設(shè)計(jì)與優(yōu)化思考
        民用建筑防煙排煙設(shè)計(jì)優(yōu)化探討
        關(guān)于優(yōu)化消防安全告知承諾的一些思考
        一道優(yōu)化題的幾何解法
        做個(gè)怪怪長(zhǎng)實(shí)驗(yàn)
        可能是方法不對(duì)
        NO與NO2相互轉(zhuǎn)化實(shí)驗(yàn)的改進(jìn)
        實(shí)踐十號(hào)上的19項(xiàng)實(shí)驗(yàn)
        太空探索(2016年5期)2016-07-12 15:17:55
        用對(duì)方法才能瘦
        Coco薇(2016年2期)2016-03-22 02:42:52
        久久精品国产亚洲av无码偷窥| 亚洲高清在线观看免费视频| 亚洲精品av一区二区日韩| 草逼短视频免费看m3u8| 性高朝大尺度少妇大屁股| 93精91精品国产综合久久香蕉| 蜜桃成人永久免费av大| 日韩精品一二三区乱码| av无码av天天av天天爽| 欧美做受视频播放| 东风日产系列全部车型| 国产亚洲精品视频一区二区三区| 久久久久亚洲av成人网人人网站| 就去吻亚洲精品欧美日韩在线| 中文字幕精品一区二区日本| 国产一区二区精品亚洲| 国语对白做受xxxxx在线| 精品国产群3p在线观看| 亚洲一区二区三区新视频| 少妇被猛烈进入到喷白浆| 艳妇臀荡乳欲伦交换在线播放| 欧美三级超在线视频| 少妇精品偷拍高潮少妇在线观看| 一本久久综合亚洲鲁鲁五月天| 亚洲中久无码永久在线观看同| 国产精品国产三级国产AvkTV| 日韩精品视频免费在线观看网站| 中文字幕在线日亚洲9| 在线观看国产高清免费不卡黄| 人妻熟女中文字幕在线视频| 国产自拍精品一区在线观看| 亚洲成人av在线蜜桃| 少妇厨房愉情理伦bd在线观看| 美女啪啪国产| 少妇又色又爽又刺激的视频| 在线无码中文字幕一区| 婷婷成人基地| 日本女优一区二区在线免费观看| 日本三区在线观看视频| 亚洲欧洲国产成人综合在线| 岛国av无码免费无禁网站下载|