馬雪梅，馬文兵

中華小苦荬萜類化學成分的研究

馬雪梅*，馬文兵

中北大學化工與環(huán)境學院，太原030051

從中華小苦荬全草的乙酸乙酯提取物中分離得到8個萜類化合物，通過波譜方法及文獻對照分別鑒定為β-香樹脂素(1)，3β-羥基-20(30)-蒲公英甾烯(2)，熊果-12-烯-3β-醇(3)，羽扇豆醇(4)，10-羥基艾里莫芬-7 (11)-烯-12，8α-內(nèi)酯(5)，烏蘇-12，20(30)-二烯-3β，28-二醇(6)，3β，8α-二羥基-6β-當歸?；锬?7(11)-烯-12，8β-內(nèi)酯(7)和烏蘇酸(8)，化合物1～8均首次從該植物中分離得到。

中華小苦荬;萜類;化學成分

Introduction

Ixeridium chinense，a herbaceous perennial plant belonging to the Compositae family，is widly distributed in Shanxi，Shandong and Henan provinces of China growing at230-4700 m above sea level．It is valued as delicious and nutritional potherb［1-3］．Up to now，its chemical constituents have not been investigated．In a continuing deep investigation of our studies on natural products with free-radical-screening activities and bioactive terpenoids from Ixeridium class［4］，we carried out phytochemical studies on this plant and eight terpenoid compounds were isolated from an EtOAc extract of the whole plant of I．chinense．We report herein the isolation and structural elucidation of all these compounds．

Materials and Methods

Apparatus and Materials

1H NMR，13C NMR and DEPT spectra were recorded on Varian INOVA-400 FT-NMR spectrometer．IR were recorded on Nicolet NEXUS-670 FT-IR spectrometer．Column chromatographies(CC)were performed on silica gel(200-300 mesh，Branch of Qingdao Haiyang Chemical Plant)．Melting points were recorded X-4 melting point apparatus and uncorrected．

The Ixeridium chinense were collected from Taiyuan of Shanxi province，in June 2007．It was authenticated by Professor Guo wen-ju，ShanXi Institute for Drug Control．

Extraction and Isolation

Ixeridium chinense(4．0 kg)were extracted four times with 75%EtOH at room temperature，and each time lasted three days．The combined extracts were evaporated to dryness under reduced pressure．The residue(120 g)was then suspended in H2O(1．2 L)，and extracted with petroleum ether(60-90℃)(1．2 L× 6)，ethyl acetate(1．2 L×4)and n-butanol(1．0 L ×4)，respectively．The EtOAc extraction was concentrated to a syrup(52 g)，and then subjected to chromatographic separation on a silica gel column(100-200 mesh，600 g)．The components of the mixture were chromatographyed using petroleum ether with increasing volume of acetone(v/v，from 30∶1 to 1∶1)as eluent to give seven fractions(Fr．1-Fr．7)．Rechromatography on silica gel(200-300 mesh)with petroleum ether-EtOAc(v/v，10∶1)as eluent，F(xiàn)r．1(v/v，from 30∶1 to 25∶1)yielded pure compound 1(15 mg)．Fr．2 (v/v，from 25∶1 to 20∶1)was eluted with petroleum ether-ethyl acetate(v/v=20∶1)to give crude compound 1(8 mg)and compound 2(6 mg)．Fr．3(v/v，from 20∶1 to 15∶1)was eluted with petroleum etherethyl acetate(v/v=15∶1)to give compound 3(12 mg)．Fr．4(v/v，from 15∶1 to 12∶1)gave compound 4 (9 mg)after CC on silica gel eluted with petroleum ether-ethyl acetate petroleum ether-ethyl acetate(v/v，10∶1)．Fr．5(v/v，from 12∶1 to 10∶1)was chromatographed using petroleum ether-acetone(v/v，12∶1)as eluent to afford compound 5(12 mg)．Fr．5(v/v，from 12∶1 to 10∶1)was chromatographed using petroleum ether-acetone(v/v，10∶1)as eluent to give compound 6．Fr．6(v/v，from 10∶1 to 5∶1)was chromatographed using petroleum ether-acetone(v/v，8∶1)as eluent to afford compound 7(10 mg)．Fr．7(v/v，from 5∶1 to 1∶1)afforded compound 8(19 mg)after CC on silica gel eluted with petroleum ether-acetone(v/v，2∶1)．

Identification

β-amyrin(1)Colorless needles，mp.195-197℃．1H NMR(CDCl3，400 MHz)δ:5.14(1H，t，J= 4.0 Hz，H-12)，3.22(1H，m，H-3)，0.97(3H，s，H-23)，0.79(3H，s，H-24)，0.77(3H，s，H-25)，1.00 (3H，s，H-26)，1.14(3H，s，H-27)，0.83(3H，s，H-28)，0.88(6H，s，H-29，30).13C NMR(CDCl3，100 MHz)δ:38.6(C-1)，27.2(C-2)，79.1(C-3)，38.7 (C-4)，55.1(C-5)，18.4(C-6)，32.8(C-7)，39.9(C-8)，47.7(C-9)，36.8(C-10)，23.2(C-11)，121.3(C-12)，145.5(C-13)，42.0(C-14)，26.8(C-15)，26.6 (C-16)，32.7(C-17)，48.2(C-18)，46.6(C-19)，31.6(C-20)，34.2(C-21)，37.5(C-22)，28.1(C-23)，15.6(C-24)，15.6(C-25)，16.8(C-26)，26.4 (C-27)，28.2(C-28)，33.3(C-29)，23.4(C-30).The spectral data were in accordance with those of reported［5］，then the compound 1 was identified asβ-amyrin.

3β-hydroxyl-20(30)-taraxastene(2)Colorless crystals，mp.278-280℃.1H NMR(CDCl3，400 MHz) δ:3.17(1H，m，H-3)，4.64(1H，brs，H-30β)，4.60 (1H，brs，H-30β).13C NMR(CDCl3，100 MHz)δ: 38.6(C-1)，27.4(C-2)，79.0(C-3)，38.8(C-4)，55.3(C-5)，18.3(C-6)，34.2(C-7)，14.5(C-8)，50.5(C-9)，37.1(C-10)，21.6(C-11)，26.2(C-12)，39.2(C-13)，42.2(C-14)，26.7(C-15)，38.3(C-16)，34.2(C-17)，48.7(C-18)，39.4(C-19)，154.6 (C-20)，25.6(C-21)，38.9(C-22)，27.9(C-23)，15.4(C-24)，16.3(C-25)，15.9(C-26)，14.8(C-27)，19.5(C-28)，25.5(C-29)，107.1(C-30).The data was in accordance of3β-hydroxyl-20(30)-taraxastene［6］.

Olean-12-ene-3β-ol(3)Colorless crystal，mp.307-309℃.1H NMR(CDCl3，400 MHz)δ:3.63(1H，t，J =6.6 Hz，H-3)，5.18(1H，m，H-12)，0.99(3H，s，H-23)，0.80(3H，s，H-24)，0.95(3H，s，H-25)，0.96(3H，s，H-26)，1.14(3H，s，H-27)，0.84(3H，s，H-28)，0.87(3H，s，H-29)，0.88(3H，s，H-30);13C NMR(CDCl3，100 MHz)δ:38.8(C-1)，27.5(C-2)，79.2(C-3)，38.9(C-4)，55.4(C-5)，18.5(C-6)，32.9(C-7)，39.6(C-8)，47.8(C-9)，37.4(C-10)，23.2(C-11)，121.7(C-12)，144.8(C-13)，42.0(C-14)，26.4(C-15)，26.9(C-16)，33.1(C-17)，47.5 (C-18)，46.9(C-19)，31.2(C-20)，35.1(C-21)，38.1(C-22)，28.2(C-23)，15.6(C-24)，15.7(C-25)，17.1(C-26)，26.5(C-27)，29.3(C-28)，33.3 (C-29)，23.5(C-30).The data were in accordance with those of reported［6］，then the compound 3 was identified as olean-12-ene-3β-ol.

Lupol(4)White needles，mp.208-210℃.1H NMR (CDCl3，400 MHz)β:3.18(1H，m，H-3)，0.85(3H，s，H-23)，0.76(3H，s，H-24)，0.83(3H，s，H-25)，1.03(3H，s，H-26)，0.91(3H，s，H-27)，0.75(3H，s，H-28)，4.64(1H，brs，H-29)，4.54(1H，brs，H-29')，1.12(3H，s，H-30).13C NMR(CDCl3，100 MHz)δ:36.9(C-1)，27.9(C-2)，78.9(C-3)，38.8 (C-4)，55.1(C-5)，18.2(C-6)，34.1(C-7)，40.8(C-8)，50.3(C-9)，37.25(C-10)，20.76(C-11)，25.17 (C-12)，37.23(C-13)，42.1(C-14)，27.3(C-15)，35.2(C-16)，42.5(C-17)，48.6(C-18)，47.7(C-19)，150.4(C-20)，30.5(C-21)，38.8(C-22)，27.9 (C-23)，15.3(C-24)，16.1(C-25)，15.9(C-26)，14.6(C-27)，17.3(C-28)，109.5(C-29)，20.6(C-30).The data were in accordance with those of reported［7，8］，then the compound 4 was identified as lupol.

10-hydroxyeremophil-7(11)-en-12，8α-olide(5) Colorless crystals，mp.170-172℃.IRνmarcm-1:3501，1772，675，1445;1H NMR(CDCl3，400 MHz)δ:4.85 (1H，dd，J=6.8，11.2 Hz，H-8)，2.60(1H，s，H-6α)，2.40(1H，m，H-6β)，2.12(1H，dd，J=6.8，14.2 Hz，H-9α)，1.86(1H，dd，J=11.2，14.2 Hz，H-9β)，1.72(3H，s，CH3-13)，1.75(1H，m，H-1α)，1.77(1H，m，H-1β)，1.42(1H，m，H-2α)，1.37(1H，m，H-2β)，1.43(1H，m，H-3α)，1.36(1H，m，H-3β)，1.21(1H，m，H-4)，0.96(3H，s，CH3-14)，0.80 (3H，d，J=8.4 Hz，CH3-15);13C NMR(CDCl3，100 MHz)δ:36.8(C-1)，22.5(C-2)，28.2(C-3)，32.9 (C-4)，45.4(C-5)，31.5(C-6)，160.9(C-7)，79.6 (C-8)，41.8(C-9)，76.4(C-10)，121.5(C-11)，174.7(C-12)，8.9(C-1)，15.4(C-14)，16.4(C-15)．The NMR data were identical to those of literature，the compound 5 was identified as 10-hydroxyeremophil-7(11)-en-12，8α-olide［9］.

Ursan-12，20(30)-dien-3β，28-diol(6)Colorless crystals，mp.230-231℃.1H NMR(CDCl3，400 MHz) δ:3.64(t，J=6.6 Hz，H-3)，5.14(m，H-12)，4.68 (brs，H-30)，4.58(brs，H-30')，0.75-1.60(CH3or CH2);13C NMR(CDCl3，100 MHz)δ:38.8(C-1)，27.5(C-2)，79.2(C-3)，38.9(C-4)，55.4(C-5)，18.5(C-6)，32.9(C-7)，39.6(C-8)，47.8(C-9)，37.4(C-10)，23.5(C-11)，124.7(C-12)，139.8(C-13)，43.0(C-14)，29.4(C-15)，22.9(C-16)，37.1 (C-17)，55.5(C-18)，38.9(C-19)，151.2(C-20)，32.1(C-21)，38.8(C-22)，28.2(C-23)，15.6(C-24)，15.7(C-25)，17.0(C-26)，23.5(C-27)，63.3 (C-28)，16.3(C-29)，109.5(C-30).The data were equal to those reported［10］，then the compoud 6 was identified ursan-12，20(30)-dien-3β，28-diol.

3β，8α-dihydroxyl-6β-angloxyeremophil-7(11)-en-12，8β-olide(7)Colorless crystals，mp.196-199℃.1H NMR(CDCl3，400 MHz)δ:3.78(1H，dd，J= 6.0，3.0 Hz，H-3)，6.24(1H，brs，H-6)，2.36(1H，m，H-9α)，2.09(1H，dd，J=14.2，11.2 Hz，H-9β)，1.70(3H，d，J=1.2 Hz，CH3-13)，1.26(3H，s，CH3-14)，0.94(3H，d，J=5.2 Hz，CH3-15)，6.35(1H，qq，J=6.6，1.2 Hz，H-3')，2.03(3H，dq，J=7.5，1.2 Hz，CH3-4')，1.92(3H，dq，J=6.0，1.3 Hz，CH3-5');13C NMR(CDCl3，100 MHz)δ:27.8(C-1)，29.5(C-2)，68.2(C-3)，39.9(C-4)，47.4(C-5)，71.5(C-6)，157.8(C-7)，105.6(C-8)，39.8(C-9)，37.4(C-10)，127.5(C-11)，172.7(C-12)，8.8(C-13)，20.4(C-14)，167.4(C-15)，168.2(C-1')，128.5(C-2')，140.2(C-3')，21.9(C-4')，15.4(C-5').The data were in accordance with those of literature［9］，then the compound 7 was identified as 3β，8αdihydroxyl-6β-angloxyeremophil-7(11)-en-12，8β-olide.

Ursolic acid(8)White powder，240-242℃．1H NMR(DMSO-d6，400 MHz)δ:8．34(1H，s，COOH)，5．16(2H，t，H-12)，3.18(2H，m，H-3);13C NMR (DMSO-d6，100 MHz)δ:38.8(C-1)，27.4(C-2)，77.8(C-3)，38.3(C-4)，54.7(C-5)，17.9(C-6)，32.8(C-7)，39.1(C-8)，37.9(C-9)，36.4(C-10)，23.2(C-11)，124.5(C-12)，138.1(C-13)，41.5(C-14)，28.1(C-15)，23.8(C-16)，46.7(C-17)，52.2 (C-18)，38.4(C-19)，38.4(C-20)，30.2(C-21)，36.2(C-22)，28.2(C-23)，15.9(C-24)，15.2(C-25)，16.9(C-26)，23.2(C-27)，178.1(C-28)，22.9 (C-29)，19.9(C-30)．The data were consistent with those of ursolic acid［11］，the compound 8 was identified as ursolic acid．

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Terpenoids from Ixeridium chinense(Thunb．)Tzvel．

MA Xue-mei*，MA Wen-bing
College of Chemical Engineering and Environment，North University of China，Taiyuan 030051，China

Eight terpenoids have been isolated from the ethyl acetate extract of Ixeridium chinense(Thunb．)Tzvel by using column chromatography and preparative thin layer chromatography．Structures for allthese compounds were proposed on the basis of spectroscopic data，together with comparing their spectral data with those of the corresponding compounds reported in the literature．They were identified asβ-amyrin(1)，3β-hydroxyl-20(30)-taraxastene(2)，olean-12-ene-3βol(3)，lupol(4)，10-hydroxyeremophil-7(11)-en-12，8α-olide(5)，ursan-12，20(30)-dien-3β，28-diol(6)，3β，8αdihydroxyl-6β-angloxyeremophil-7(11)-en-12，8β-olide(7)and ursolic acid(8)．Compounds 1-8 were obtained from this plant for the first time．

Ixeridium chinense;terpenoids;chemical constituents

October 9，2009;Accepted January 15，2010

book=2011,ebook=1

R284．2;Q946．91

A

1001-6880(2011)03-0440-04

*Corresponding author Tel:86-351-3629665;E-mail:maxuemei@live．nuc．edu．cn