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        Correction to: Overexpression of PtPEPCK1 gene promotes nitrogen metabolism in poplar

        2022-11-04 09:12:20LinaWangMiaoHeSongChenKeanWangDonghaiCuiXinHuangLijieLiu
        Journal of Forestry Research 2022年6期

        Lina Wang · Miao He · Song Chen · Kean Wang ·Donghai Cui · Xin Huang · Lijie Liu

        Correction to: J. For. Res.https:// doi. org/ 10. 1007/ s11676- 019- 01042-4

        In the Original publication, the author has found that C1 and C4 in Fig. 3 have been published with errors. The corrected Fig. 3 C1 and C4 are provided below.

        Fig. 3 Regeneration of Agrobacterium- mediated transformed Populus by the dark-induced and light-induced methods; A1 co-cultivation of leaf discs with Agrobacterium on MS medium in the dark; A2- B2 callus induction in 84 K poplar from leaves cultured on MS medium treated with various concentrations and combinations of 2,4-D, KT,and TDZ hormones after 8-10 weeks at 25 ± 2 °C under dark conditions; B3-B4 shoot regeneration from well-developed callus from diff erent media; B3 elongated shoots from clusters which had attained 2-3 cm in length; B4 elongation period: transgenic plantlets forming roots within 2 weeks in kanamycin-containing rooting medium and acclimatization period; C1 and C2 co-cultivation of leaf discs with Agrobacterium on MS medium in light and leaf explants cultured on selected media with 50 mg·L - 1 kanamycin and 300 mg·L - 1 cefotaxime for 3-4 weeks after co-cultivation; C3 regenerated transformants on MS medium; C4 regenerated transformants on MS medium generated with the optimization of transformation effi ciency; kana-resistant shoots were further elongated in the medium and buds started appearing in clusters; D1- D2 regeneration of plantlets in MS medium; D3 transgenic plantlets forming roots within 2 weeks in rooting medium containing kanamycin; D4 acclimatization period

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