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        Isolation, Crystal Structure and Antitussive Activity of 9S,9aS-neotuberostemonine①

        2018-05-11 11:20:40WUYiYEQingMeiLIUJingXUWeiZHUZiRongJIANGRenWng
        結(jié)構(gòu)化學 2018年4期

        WU Yi YE Qing-Mei LIU Jing XU Wei ZHU Zi-Rong JIANG Ren-Wng③

        a (Zhongshan Torch Polytechnic, Zhongshan 528436, China)

        b (Department of Pharmacy, Hainan General Hospital, Haikou 570311, China)

        c (Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, College of Pharmacy,Jinan University, Guangzhou 510632, China)

        1 INTRODUCTION

        The root of three species of Stemona has long been used in Chinese traditional medicine as antitussive agents and insecticides[1-2].Plants in this genus have attracted many phytochemical[3-5]and pharmacological interests[6-8]and over eighty alkaloids have been isolated[9].These alkaloids could be classified into six structural groups according to the structural skeleton[10].

        We have been engaged in the identification of structurally unique alkaloid from Stemona species[11-15], assessment of their antiussive effects[16],and investigation of the in depth mechanism[17].Our current phytochemical study on the chemical constituents of Stemona tuberosa collected in Guangxi province led to the isolation of 9S,9aS-neotuberostemonine (1, scheme 1), which was a new isomer of our previously reported tuberostemonine (2), tuberostemonine D (3), neotuberostemonine (4) and tuberostemonine K(5)[14].Compound 1 was found to show antitussive activity in a dose-dependent manner.Compound 1 has ten chiral centers, and a precise understanding of their three-dimensional structures would be important for understanding the bioactivities.We report herein the isolation, crystal structure and antitussive activity of compound 1.

        Scheme 1. Structure of compound 1, tuberostemonine (2), tuberostemonine D (3),neotubersostemonine (4) and tubersostemonine K(5)

        2 EXPERIMENTAL

        2.1 Materials and instrumentations

        Optical rotations were recorded on a Perkin-Elmer 341 Polarimeter in MeOH solution.Melting points were determined using a Fisher scientific and uncorrected.The UV spectra were obtained on a Beckman Du650 spectrophotometer in MeOH.IR spectra were recorded on a Nicolet impact 420 FT-IR spectrometer.The NMR spectra were obtained on a Bruker 300 spectrometer with chemical shift reported in δ (ppm) using TMS as the internal stand.ESIMS was recorded on a Finnigan MAT TSQ 7000 instrument.X-ray diffraction of compound 1 was conducted on a Bruker SMART1000 CCD diffractometer.

        2.2 Plant material

        Roots of Stemona tuberosa were collected in Guangxi province, and were identified by Prof.Guangzhou Zhou in College of Pharmacy, Jinan University.A voucher specimen (ST-GX1) is deposited in College of Pharmacy, Jinan University.

        2.3 Extraction and isolation

        Dry ground roots of Stemona tuberosa (1 kg) were refluxed with 95% ethanol.After the evaporation of ethanol, the residue was acidified with dilute HCl(4%) and centrifuged at 5 °C, 3500RPM for 30 min.The supernatant was basified with aqueous amonia to pH = 9 and extracted with Et2O to afford the total alkaloid (6.3 g).The total alkaloids were subjected to silica gel chromatography; eluted with gradient solvent of CHCl3:MeOH:NH4OH to give compound 1, which was further purified by re-crystallization from hexane and ethyl acetate mixture (2:1) at room temperature.

        2.4 Spectral structure determination

        Compound 1: C22H33NO4, m.p.: 183~185 ℃,+77.6° (c 0.1 in CH3OH); IR (KBr) vmax2945,1769, 1454, 1174 and 1016 cm-1.ESI-MS 375[M]+,276[M-C5H7O2]+.1H NMR (CD3OD, 300 MHz) δ:1.79 [m, H(1)], 1.57 [m, H2(2)], 3.20 [dd, J = 6.5,11.8 Hz, H(3)], 2.61 [m, H(5α)], 2.83 [m, H(5β)],1.42 [m, H(6α)], 1.60 [m, H(6β)], 1.43 [m, H(7α)],1.82 [m, H(7β)], 1.63 [m, H(8α)], 1.93 [m,H(8β],1.85 [m, H(9)], 3.01 [dd, J = 6.6, 7.2 Hz,H(9a)], 1.71 [m, H(10)], 4.57 [dd, J = 4.3, 3.6 Hz,H(11)], 1.97 [m, H(12)], 2.75 [dq, J = 6.5, 12.3 Hz,H(13)], 1.18 [d, J = 6.5 Hz, H3(15)], 1.38 [m,H(16α)], 1.67 [m, H(16β)], 1.02 [t, J = 7.2 Hz,H3(17)], 4.37 [ddd, J = 4.6, 6.5, 11.8 Hz, H(18)],1.43 [m, H(19α)], 2.35 [ddd, J = 4.1, 6.5, 14.5 Hz,H(19β)], 2.46 [ddd, J = 5.2, 7.2, 10.8 Hz, H(20)],1.22 [d, J = 7.2 Hz, H3(22)].13C NMR [CD3OD, 75 MHz] δ: 41.9 [C(1)], 31.2[C(2)], 78.0[C(3)],54.7[C(5)], 27.3[C(6)], 24.1[C(7)], 27.1[C(8)],41.1[C(9)], 67.5[C(9a)], 35.3[C(10)], 80.7[C(11)],44.1[C(12)], 47.2[C(13)], 179.5[C(14)], 11.6[C(15)],21.2[C(16)], 11.9[C(17)], 79.2[C(18)], 33.4[C(19)],44.8[C(20)], 179.1[C(21)], 15.1[C(22)].

        2.5 X-ray structure determination

        The crystals suitable for X-ray structure determination were obtained by slow evaporation of hexane and ethyl acetate mixture (2:1) at room temperature.A colorless prism-like crystal of the title compound with dimensions of 0.38mm × 0.36mm ×0.24mm was selected and mounted on a thin glass fiber.Intensity data were collected at room temperature (298 K) on a Smart1000 CCD diffractometer using MoKα radiation λ = 0.71079 ?.The data frames with a maximum 2θ value of ~52owere processed using the program SAINT.The data were corrected for absorption and beam corrections based on the multi-scan technique as implemented in SADABS.

        A total of 3109 reflections were collected in the range of 1.85≤θ≤26.00° (index ranges: –1≤h≤11,–1≤k≤13, –1≤l≤27) by using an ω scan mode, of which 2902 independent reflections (Rint= 0.0133)with I > 2σ(I) were considered as observed and used in the succeeding refinements.The structure was solved by direct methods with SHELXS-97 and expanded by using Fourier difference techniques.The non-hydrogen atoms were refined anisotropically,and the hydrogen atoms were added according to theoretical models.The structure was refined by full-matrix least-squares techniques on F2with SHELXL-97.The final refinement gave R = 0.0579,wR = 0.1358 (I > 4σ(I)), w = 1/[σ2(Fo2) + (0.0743P)2+ 0.5547P], where P = (Fo2+ 2Fc2)/3.(Δ/σ)max=0.000, S = 1.019, (Δρ)max= 0.457 and (Δρ)min=–0.235 e/?3.

        2.6 Antitussive assay

        Antitussive assay was carried out as previously reported[18].Briefly, unrestrained conscious guinea pigs were individually placed in a transparent Perspex airtight chamber and exposed to 0.5 M citric acid aerosol produced by an ultrasonic nebulizer(NEU12, Omron, Tokyo, Japan) for 10 min with a flow rate of 0.6 mL/min.During the 10 min observation period, the animals were continuously observed.Animals producing cough more than 18 times but less than 30 in the first challenge were selected for further antitussive tests.Cough episodes during the first challenge were recorded as the control data.After 72 h recovery, the selected sensitive animals were randomly divided into several groups with six animals in each group.Compound 1 was suspended in 0.1% CMC.The samples were intragastric ally administered at 60 min before the second challenge.

        Table 1. Selected Bond Lengths (?) and Bond Angles (°)

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        Table 2. Hydrogen Bond Lengths (?) and Bond Angles (°)

        3 RESULTS AND DISCUSSION

        Compound 1 was obtained by silica gel column chromatography of the 95% ethanol extract followed by open silica gel column chromatography, and recrystallized as colorless prism-like crystals from hexane-ethyl acetate mixture (2:1).High resolution ESIMS analysis of 1 showed a quasi-molecular ion peak at [M+H]+376.2476, corresponding to a molecular formula C22H33NO4(calculated 376.2482).Its IR spectrum (KBr) showed the presence of a γ-lactone ring (1763 cm-1).The ESI mass spectrum showed [M+H]+at m/z 376 and the base peak at m/z 276 [M-C5H7O2] indicated the presence of the typical β-methyl-γ-lactone ring annexed to C(3) of the azepine ring.

        The1H-NMR spectrum of compound 1 showed a triplet (3H) at δ 1.02 for the C(17) methyl group and two doublets (3H each) at δ 1.18 and 1.22, corresponding to two secondary methyl groups at C(13)and C(20).The13C-NMR spectrum showed two lactone carbonyls at δ179.10 and 179.45, corresponding to C(21) and C(14).These signals indicated that compound 1 belongs to the tuberostemonine-type of alkaloids[14].

        The complete structure and stereochemistry were determined unambiguously by X-ray diffraction analysis.Selected bond lengths and bond angles of compound 1 are given in Table 1.Fig.1 shows the molecular structure of the title compound, and Fig.2 depicts the packing diagram.

        The crystal belongs to orthorhombic system with space group P212121.The crystal data are listed Table 1.The skeleton consists of two γ-lactone rings, a pyrrolidine, a cyclohexane, and an azepine ring.The lactone rings D and E adopt an envelope conformation with C(12) and C(19) displaced by 0.607 and 0.350 ? from the corresponding least-squares plane of the remaining four atoms, respectively.The pyrrolidine ring A fused to the cyclohexane and azepine rings has a twist envelope conformation.The cyclohexane ring has a distorted chair conformation as indicated by the smaller torsion angle C(10)–C(11)–C(12)–C(1) 39.3°.The azepine ring adopts a chair conformation.The groups of atoms C(5), N(4),C(8) and C(9) form a plane with mean deviation of 0.0035 ?.The deviations of C(9A), C(6) and C(7)from this plane are –0.6403, 1.1532 and 1.0563 ?,respectively.The stereochemisty of ring juncture is A/B trans, B/C trans, A/C trans and C/D cis.The configurations at the ten chiral centers are determined as follows: H(1), H(9), H(18) and H(20) are β-oriented.H(3), H(9a), H(10), H(11), H(12) and H(13) are α-oriented.

        Accordingly, the relative configurations of the chiral centers C(1), C(3), C(9), C(9a), C(10), C(11),C(12), C(13), C(18) and C(20) were established to be rel-(S, S, S, S, R, S, S, S, S and S), respectively.Compound 1 is an isomer of neotuberostemonine[14]at C(9) and C(9a).It is here named 9S,9aS-neotuberostemonine.Compound 1 is also an isomer of tuberostemonine[14]at C(1), C(9), C(9a), C(11) and C(12), and an isomer of tuberstemonine K[14]at C(1),C(9), and C(9a).Due to the absence of heavy atom in the molecule, the final refinement resulted in a non-significant Flack parameter 0(3); however, con-sidering the conserved C(13) β- and C(20) α-orientated methyl groups in Stemona alkaloids[9,10], the absolute configuration of compound 1 could be assigned as shown in Fig.1.

        Fig.1. Molecular structure of 1 showing 30% probability displacement ellipsoids and the atom-numbering scheme

        Normally, drug molecules exert pharmacological effects in solution state.It is necessary to compare the conformations in the solution and solid state.The1H-NMR spectrum of 1 showed that the coupling constants between H(11) and H(12) and H(10) are 4.3 and 3.6 Hz, respectively.These coupling constants are consistent with the torsion angles H(11)–C(11)–C(12)–H(12) of 42.3° and H(10)–C(10)–C(11)–H(11) of 54.5° in the crystal structure.Thus,the conformation of 1 in methanol might be consistent with that in crystalline state, which is similar to 1β-hydroxydigitoxigenin[19].

        In solid state, the molecules were linked into a chain along the a-axis through weak hydrogen bond C(11)-H(11A)…O(2) (3.482(4) ?, symmetry code:x+0.5, 0.5–y, –z), as shown in Fig.2.

        Fig.2. A packing diagram for compound 1.Hydrogen-bonding network of 1 viewed roughly down the b-axis.Selected hydrogen atoms highlight the scheme of hydrogen bonding

        The antitussive property of 1 was studied using a citric acid induced guinea pig cough model.Compound 1 showed significant inhibition of cough by 24%, 44% and 65% at doses of 50, 100 and 150 mg/kg, respectively, which is comparable to the positive control codeine at 15 mg/kg (68% cough reduction).The potency of 1 was lower than the reported value of neotuberostemonine (4, 85%inhibition at 50 mg/kg)[20], but much stronger than epi-bisdehydrotuberostemonine J (no significant antitussive activity)[19], indicating that the configurations at C(9) and C(9a) and the presence of double bonds in the pyrrole ring influence the bioactivity.It is noteworthy that codeine is commonly used as positive control in antitussive studies.Though it has potent antitussive effect, its application in clinics is limited due to the strong addiction side effect.Discovery of the antitussive effect of 9S,9aS-neotuberostemonine (1) partially accounts for the application of Stemona species in Chinese traditional medicine; however, structural modification is warranted to further improve the potency.

        REFERENCES

        (1) Pharmacopoeia Commission of People’s Republic of China.The Pharmacopoeia of the People’s Republic of China, Part 1.Chemical Industry Publishing House, Beijing, China 2005, 100.

        (2) Jiangsu New Medical College.Dictionary of Chinese Traditional Medicine.Shanghai People’s Publishing House, China 1977, 858–861.

        (3) Lin, L.G.; Leung, H.P.H.; Zhu, J.Y.; Tang, C.P.; Ke, C.Q.; Rudd, J.A.; Lin, G.; Ye, Y.Croomine- and tuberostemonine-type alkaloids from roots ofStemona tuberosaand their antitussive activity.Tetrahedron2008, 64, 10155–10161.

        (4) Ye, Y.; Qin, G.W.; Xu, R.S.Alkaloids of Stemona japonica.J.Nat.Prod.1994, 57, 665–669.

        (5) Yang, X.Z.; Zhu, J.Y.; Tang, C.P.; Ke, C.Q.; Lin, G.; Cheng, T.Y.; Rudd, J.A.; Ye, Y.Alkaloids from roots ofStemona sessilifoliaand their antitussive activities.Planta Med.2009, 75, 174–177.

        (6) Wu, Y.X.; He, H.Q.; Nie, Y.J.; Ding, Y.H.; Sun, L.; Qian, F.Protostemonine effectively attenuates lipopolysaccharide-induced acute lung injury in mice.Acta Pharmacol.Sin.2018, 39, 85–96.

        (7) Umsumarng, S.; Pitchakarn, P.; Yodkeeree, S.; Punfa, W.; Mapoung, S.; Ramli, R.A.; Pyne, S.G.; Limtrakul, P.Modulation of P-glycoprotein by Stemona alkaloids in human multidrug resistance leukemic cells and structural relationships.Phytomedicine 2017, 34,182–190.

        (8) Sakulpanich, A.; Attrapadung, S.; Gritsanapan, W.Insecticidal activity of Stemona collinsiae root extract against Parasarcophaga ruficornis (Diptera: Sarcophagidae).Acta Trop.2017, 173, 62–68.

        (9) Greger, H.Structural relationships, distribution and biological activities of Stemona alkaloids.Planta Med.2006, 72, 99–113.

        (10) Pilli, R.A.; Rossoa, G.B.; Oliveira, M.C.The chemistry of Stemona alkaloids: An update.Nat.Prod.Rep.2010, 27, 1908–1937.

        (11) Jiang, R.W.; Hon, P.M.; But, P.P.H.; Chung, H.S.; Lin, G.; Ye, W.C.; Mak, T.C.W.Isolation and stereochemistry of two new alkaloids fromStemona tuberoseLour.Tetrahedron2002, 58, 6705–6712.

        (12) Jiang, R.W.; Hon, P.M.; Xu, Y.T.; Chan, Y.M.; Xu, H.X.; Shaw, P.C.; But, P.P.H.Isolation and chemotaxonomic significance of tuberostemospironine-type alkaloids from Stemona tuberosa.Phytochemistry2006, 67, 52–57.

        (13) Zhang, R.R.; Tian, H.Y.; Wu, Y.; Sun, X.H.; Zhang, J.L.; Ma, Z.G.; Jiang, R.W.Isolation and chemotaxonomic significance of stenine- and stemoninine-type alkaloids from the roots of Stemona tuberosa.Chin.J.Struct.Chem.2014, 25, 1252–1255.

        (14) Jiang, R.W.; Hon, P.M.; Zhou, Y.; Chan, Y.M.; Xu, Y.T.; Xu, H.X.; Greger, H.; Shaw, P.C.; But, P.P.H.Alkaloids and chemical diversity ofStemona tuberosa.J.Nat.Prod.2006, 69, 749–754.

        (15) Jiang, R.W.; Ye, W.C.; Shaw, P.C.; But, P.P.H.; Mak, T.C.W.Absolute configuration of neostenine.J.Mol.Struct.2010, 966, 18–22.

        (16) Yi, M.; Xia, X.; Wu, H.Y.; Tian, H.Y.; Huang, C.; But, P.P.H.; Shaw, P.C.; Jiang, R.W.Structures and chemotaxonomic significance of stemona alkaloids from Stemona japonica.Nat.Prod.Commun.2015, 10, 2097–2099.

        (17) Xu, Y.T.; Hon, P.M.; Jiang, R.W.; Cheng, L.; Li, S.H.; Chan, Y.P.; Xu, H.X.; Shaw, P.C.; But, P.P.H.Antitussive effects ofStemonatuberosa with different chemical profiles.J.Ethnopharmacol.2006, 108, 46–53.

        (18) Xu, Y.H.; Xu, J.; Jiang, X.Y.; Chen, Z.H.; Xie, Z.J.; Jiang, R.W.; Feng, F.Isolation, crystal structure and Na+/K+-ATPase inhibitory activity of 1β-hydroxydigitoxigenin.Chin.J.Struct.Chem.2016, 35, 1024–1030.

        (19) Xu, Y.T.; Shaw, P.C.; Jiang, R.W.; Hon, P.M.; Chan, Y.M.; But, P.P.H.Antitussive and central respiratory depressant effects of Stemona tuberosa.J.Ethnopharmacol.2010, 128, 679–684.

        (20) Chung, H.S.; Hon, P.M.; Lin, G.; But, P.P.; Dong, H.Antitussive activity of Stemona alkaloids from Stemona tuberosa.Planta Med.2003, 69, 914–920.

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