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        兒童新甲型(H1N1)流感疫苗接種后遠(yuǎn)期疫苗活化Th1/Th2細(xì)胞因子分泌水平分析

        2017-05-17 08:36:59夏洪波陳珺汝郭錦錦
        中國(guó)當(dāng)代醫(yī)藥 2017年7期
        關(guān)鍵詞:兒童

        夏洪波+陳珺汝+郭錦錦

        [摘要]目的 檢測(cè)兒童新甲型(H1N1)流感疫苗接種后其遠(yuǎn)期疫苗活化Th1/Th2細(xì)胞因子分泌水平的變化情況。方法 選取本院2009年12月~2010年1月收治的自愿接受新甲型H1N1流感疫苗接種的31名健康兒童,取3 ml外周血分離淋巴細(xì)胞,分別設(shè)新甲型H1N1流感疫苗刺激組(實(shí)驗(yàn)組)和正常細(xì)胞組(對(duì)照組),進(jìn)行細(xì)胞培養(yǎng)后,采用流式CBA法及ELISA法檢測(cè)Th細(xì)胞因子分泌水平。結(jié)果 流式CBA技術(shù)檢查顯示,試驗(yàn)組的TNF-α、IL-10水平明顯低于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P<0.05)。ELISA方法檢測(cè)顯示,兩組的TNF-α、IL-4水平比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P>0.05);實(shí)驗(yàn)組的IL-10水平顯著低于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P<0.05)。對(duì)照組和實(shí)驗(yàn)組流式CBA方法檢測(cè)的IL-10 水平明顯高于ELISA方法檢測(cè)結(jié)果,差異有統(tǒng)計(jì)學(xué)意義(P<0.05),且兩種方法檢測(cè)結(jié)果的相關(guān)性具有統(tǒng)計(jì)學(xué)意義,分別得到回歸方程Y=206.59+0.082X和Y=136.70+0.168X。結(jié)論 新甲流疫苗再次刺激未能很好地活化Th1及Th2細(xì)胞,即未產(chǎn)生較好的Th1型細(xì)胞免疫反應(yīng)和較好的體液免疫,提示新甲流疫苗特異性免疫記憶功能欠佳;流式CBA方法與傳統(tǒng)ELISA方法檢測(cè)細(xì)胞因子均具有較高的靈敏度且具有相同的結(jié)果規(guī)律。

        [關(guān)鍵詞]新甲型H1N1流感疫苗;Th1/ Th2細(xì)胞因子;兒童;免疫記憶

        [中圖分類(lèi)號(hào)] R511.7 [文獻(xiàn)標(biāo)識(shí)碼] A [文章編號(hào)] 1674-4721(2017)03(a)-0011-05

        [Abstract]Objective To test the changes of secretion levels of long-term vaccine activation of Th1/Th2 cytokine after new influenza A (H1N1) vaccination in children.Methods A total of 31 healthy children who were on their own willing for new influenza A (H1N1) vaccination from December 2009 to January 2010 in our hospital were selected,lymphocyte cells were separated from 3 ml peripheral blood for culture,and then were divided into new influenza A (H1N1) stimulation group (the experimental group) and normal cell group (the control group).The levels of Th cytokine secretion were measured by flow CBA and enzyme-linked immunosorbent assay (ELISA).Results The technical examination of the flow CBA showed that the levels of TNF-α and IL-10 in the experimental group was significantly lower than those in the control group,with significant difference (P<0.05).The ELISA method displayed no significant difference in the level of TNF-α and IL-4 between the experimental group and the control group (P>0.05).The level of IL-10 in the experimental group was significantly lower than that in the control group,which was in statistical differences (P<0.05).The levels of IL-10 in both the control group and the experimental group tested by flow CBA was significantly higher than those by the ELISA test,with statistical differences (P<0.05).The correlation of test outcomes by the two methods was displayed a statistical significance in equation of Y=206.59+0.082X and Y=136.70+0.168X respectively.Conclusion Re-stimulation of new influenza A H1N1 vaccine cannot activate Th1 and Th2 cells in a great success,that′s to say,no satisfactory Th1 cellular immune response and humoral immunity suggesting that the specific immune memory function of the new influenza A H1N1 is poor.The higher sensitivity and the same test results are obtained by flow CBA and the traditional ELISA method in detecting cytokines.

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