Zi-ru YU,Li LI,Jin-hua WANG,Zhen-zhong WANG,Wei XIAO,Guan-hua DU

(1.Beijing Key Laboratory of Drug Targets Identification and Drug Screening，Institute of Materia Medica，Chinese Academy of Medical Sciences and Peking Union Medical College，Beijing 100050，China;2.State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process,Jiangsu Kanion Pharmaceutical Co,Ltd,Lianyungang 222001,China)

T2-40

Effect of Guizhi Fuling capsule and its extracts on human breast cancer cells proliferation

Zi-ru YU1,Li LI1,Jin-hua WANG1,Zhen-zhong WANG2,Wei XIAO2,Guan-hua DU1

(1.Beijing Key Laboratory of Drug Targets Identification and Drug Screening，Institute of Materia Medica，Chinese Academy of Medical Sciences and Peking Union Medical College，Beijing 100050，China;2.State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process,Jiangsu Kanion Pharmaceutical Co,Ltd,Lianyungang 222001,China)

OBJECTIVETo evaluate the effect of Guizhi Fuling Capsule active pharmaceutical ingredient(API)and its fractions on human breast cancer cells proliferation by high-throughput screening assay.METHODSThe crude fractions were obtained from the extraction and elution of the API of Guizhi Fuling Capsule,and 929 standard fractions were obtained by the optimal separation conditions.Sulforhodamine B(SRB)method was used to evaluate the effects of the Guizhi Fuling capsule API and 929 kinds of fractions on the proliferation of human breast cancer cells MCF-7 and MDA-MB-231.RESULTSThe Guizhi Fuling capsule API had a strong ability to inhibit the proliferation of MCF-7 cells at high concentration and the ability to inhibit MDA-MB-231 cells′proliferate at low concentration follow?ing 72 h treatment；some samples of 929 fractions(5 μg·mL-1)was found to have a breast cancer cell growth inhibition rate above 50%,without toxicity on HUVECs proliferation.CONCLUSIONThe API of Guizhi Fuling capsule had significant cytotoxicity effects on these two human breast cancer cells,with significant concentration-and time-dependent manner.

high throughput screening;Guizhi Fuling capsule;breast cancer;MCF-7 cell;MDAMB-231 cell;sulforhodamine B

The project supported by National Science and Technology Major Projects of China（2013ZX09402203,2013ZX09508104）；Medical and Health Science and Technology Innovation Engineering of Chinese Academy of Medical Sciences（2016-I2M-3-007);and National Natural Science Foundation of China（81573454）

Guan-hua DU,Tel:(010)63165184,E-mail：dugh@imm.ac.cn