張?jiān)?/p>

摘 要：當(dāng)前，微生物發(fā)酵過(guò)程研究正逐漸由胞外深入到胞內(nèi)，由宏觀擴(kuò)展到微觀。建立能檢測(cè)并綜合利用細(xì)胞內(nèi)外的宏觀和微觀代謝信息的方法非常必要。我們探討并建立了微觀和宏觀相結(jié)合的綜合檢測(cè)和分析方法。同時(shí)我們還建立了利用通過(guò)同位素標(biāo)記實(shí)驗(yàn)，對(duì)發(fā)酵過(guò)程階段的代謝通量的精確分析。

關(guān)鍵詞：細(xì)胞代謝 微生物發(fā)酵 代謝工程 高通量篩選

Abstract：Industrial systems bioprocess engineering by integrating the multi scale physiological metabolism information is more essential. In the present work， we established the methods for determing and analyzing the macro- and micro-metabolic information. In addition， the 13C-labeling experiments were developed and all the online parameters reflecting the physiological characters can be monitored and controlled.

Key Words：Metabolic bioprocess；Metabolic engineering；High-throughput screening；Microbial fermentation

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