Shaofen Huang， Jiajian Liang， Gary Hin-Fai Yam， Zhihao Lu， Chi Pui Pang，2， Haoyu Chen，2，*

1 Joint Shantou International Eye Center，Shantou University＆ Chinese University of Hong Kong，Shantou，China

2 Department of Ophthalmology and Visual Sciences， Chinese University of Hong Kong， Hong Kong， China

3 Singapore Eye Research Institute，Singapore

Introduction

Materials and methods

The mouse model of oxygen-induced retinopathy

Intravitreal injection of the GSI-B4 Alexa Fluor 568 conjugate

Cardiac infusion of the FITC-dextran conjugate

Retinal whole mounts

Measurements of retinopathy and statistical analysis

Results

Figure 1 Retinal flat-mount of oxygen-induced retinopathy mouse model．A．D．G： FITC-dextran perfusion； B．E．H： isolectin staining； C．F．I： coincidence of the two measures； A-C： overview of the retinal flat-mount； D-F： some areas stained with isolectin but not perfused with FITC dextran．G-I： some areas perfused with FITC dextran but not stained with isolectin．

Discussion

Figure 2 Bland-Altman plot of the retinal neovascularization area in the oxygen induced retinopathy mouse model measured by FITC-dextran perfusion and isolectin staining．