夏海威，施國(guó)新，黃 敏，吳 娟

南京師范大學(xué)生命科學(xué)學(xué)院 江蘇省生物多樣性與生物技術(shù)重點(diǎn)實(shí)驗(yàn)室, 南京 210023

一氧化氮對(duì)植物重金屬脅迫抗性的影響研究進(jìn)展

夏海威，施國(guó)新*，黃 敏，吳 娟

南京師范大學(xué)生命科學(xué)學(xué)院 江蘇省生物多樣性與生物技術(shù)重點(diǎn)實(shí)驗(yàn)室, 南京 210023

一氧化氮(NO)作為一種重要的信號(hào)分子，在調(diào)節(jié)植物重金屬脅迫抗性方面上起著非常重要的作用。綜述了NO在植物體內(nèi)的產(chǎn)生途徑，重金屬脅迫下植物體內(nèi)內(nèi)源NO含量的變化以及外源NO與內(nèi)源NO對(duì)植物重金屬脅迫抗性的影響。大量研究表明外源NO能夠增強(qiáng)植物對(duì)重金屬脅迫的抗性，一方面是通過(guò)增強(qiáng)植物細(xì)胞的抗氧化系統(tǒng)或直接清除活性氧，另一方面是通過(guò)影響植物對(duì)重金屬的吸收以及重金屬在植物細(xì)胞內(nèi)的分布。然而內(nèi)源NO在調(diào)節(jié)植物重金屬脅迫抗性上的功能角色仍存在爭(zhēng)議。有些研究表明內(nèi)源NO是有益的，能夠緩解重金屬脅迫誘導(dǎo)的毒性；但是也有證據(jù)表明內(nèi)源NO是有害的，能夠通過(guò)促進(jìn)植物對(duì)重金屬的吸收以及對(duì)植物螯合素進(jìn)行S-亞硝基化弱化其解毒功能，從而參與重金屬誘導(dǎo)的毒害反應(yīng)和細(xì)胞凋亡過(guò)程。

外源NO; 內(nèi)源NO; 重金屬脅迫; 抗性

一氧化氮(Nitric Oxide, NO)是一種廣泛存在于生物體內(nèi)的水溶性和脂溶性氣體小分子信號(hào)物質(zhì)，在植物體內(nèi)參與多種生理過(guò)程，如誘導(dǎo)種子萌發(fā)[1]、抑制種子休眠[2], 調(diào)節(jié)植物光合作用[3]、花的形成[4]以及植物的根向地性生長(zhǎng)[5]，延緩植物的衰老過(guò)程[6]等。同時(shí)，NO也參與調(diào)節(jié)植物對(duì)一系列非生物和生物脅迫的抗性，在非生物脅迫抗性方面，NO能夠增強(qiáng)植物抗鹽性，抗旱性，抗?jié)承?，抗極端溫度，抗紫外線輻等[7]；在生物脅迫抗性方面，NO能通過(guò)增加3′5′-環(huán)-磷酸鳥(niǎo)苷(cGMP)和水楊酸(SA)的水平來(lái)增強(qiáng)植物的抗病性[8]。

對(duì)重金屬脅迫抗性而言，已有大量文獻(xiàn)表明NO能夠調(diào)節(jié)植物對(duì)重金屬脅迫抗性，但對(duì)NO在植物重金屬脅迫抗性方面的具體機(jī)制做系統(tǒng)總結(jié)和概括的研究較少并且對(duì)內(nèi)源NO在調(diào)節(jié)植物重金屬脅迫抗性上的功能角色也很少涉及。本文結(jié)合國(guó)內(nèi)外最新的研究進(jìn)展，綜述了NO調(diào)節(jié)植物重金屬脅迫抗性的相關(guān)機(jī)制，為研究植物重金屬脅迫抗性機(jī)制提供了參考。

1 植物體內(nèi)NO的產(chǎn)生途徑

1.1 酶促反應(yīng)途徑

1.1.1 一氧化氮合酶(NOS)途徑

在動(dòng)物體內(nèi)，NO主要通過(guò)NOS以L-精氨酸、O2及NADPH為底物催化而成，F(xiàn)AD、FMN、血紅素、四氫葉酸、Ca2+/CaM和Zn2+為NOS的輔基[9]。在植物體內(nèi)也有類(lèi)似的NOS，Neill等[7]發(fā)現(xiàn)植物能夠通過(guò)氧化L-精氨酸形成瓜氨酸而產(chǎn)生NO，并且動(dòng)物NOS抑制劑L-硝基精氨酸甲酯(L-NAME)能夠抑制擬南芥NOS活性并減少NO的產(chǎn)生[10]，另外人們已經(jīng)在植物組織如豌豆的根、莖、葉中[11]以及各種細(xì)胞器如過(guò)氧化物酶體[12]和葉綠體[13]中都檢測(cè)到了NOS活性。然而植物NOS的基因和蛋白序列與動(dòng)物卻并不相同，如最近發(fā)現(xiàn)的綠藻(Ostreococcustauri)中的NOS蛋白序列已經(jīng)被鑒定出來(lái)，結(jié)果其與動(dòng)物的NOS蛋白序列僅有45%的相似性，而其結(jié)構(gòu)模型與動(dòng)物的反應(yīng)區(qū)域卻高等相似[14]，另外Guo等發(fā)現(xiàn)擬南芥中AtNOS1基因編碼的蛋白與蝸牛中參與NO合成的蛋白有相似序列，但這一蛋白與典型的動(dòng)物NOS蛋白序列沒(méi)有相似性[15]，并且體外重組的AtNOS1也沒(méi)有NOS活性[16]，實(shí)際上AtNOS1是一種GTPase而不是NOS，因而命名為AtNOA1[17]，該蛋白可能參與線粒體核糖體的生物合成以及翻譯過(guò)程，并在此過(guò)程中間接參與NO的合成[16]。

1.1.2 硝酸還原酶(NR)和亞硝酸還原酶(Ni-NOR)途徑

1.1.3 其他酶促途徑

另外，植物體內(nèi)還有其他的酶也參與NO的產(chǎn)生，如辣根過(guò)氧化物酶[21]、黃嘌呤氧化酶(XOR)以及黃嘌呤脫氫酶(XDH)[22]、細(xì)胞色素P450[23]等。

1.2 非酶促途徑

2 重金屬脅迫下植物體內(nèi)內(nèi)源NO含量的變化

重金屬脅迫下，植物細(xì)胞內(nèi)源NO含量往往會(huì)發(fā)生顯著變化，然而內(nèi)源NO含量的變化卻有眾多的影響因素。

如100 μmol/L的銅(Cu)、鎘(Cd)、鋅(Zn)短期處理24 h以后，豌豆根內(nèi)的內(nèi)源NO含量升高[27]，而100 μmol/L的Cu、Cd、Zn長(zhǎng)期處理14 d以后，豌豆根內(nèi)的內(nèi)源NO含量卻下降[28]，說(shuō)明重金屬處理時(shí)間的長(zhǎng)短能夠影響植物體內(nèi)內(nèi)源NO含量的變化。

Leterier等用不同濃度的砷(As)對(duì)擬南芥處理7 d以后發(fā)現(xiàn)，當(dāng)砷濃度小于250 μmol/L時(shí)，擬南芥根內(nèi)的NO含量沒(méi)有增加，甚至在250 μmol/L時(shí)出現(xiàn)了下降；而當(dāng)砷濃度超過(guò)500 μmol/L時(shí)，擬南芥根內(nèi)的NO含量大幅增加，并且在500 μmol/L時(shí)達(dá)到最大[29]，表明植物體內(nèi)內(nèi)源NO含量的變化與重金屬的濃度有關(guān)。

100 μmol/L的Cd處理24 h以后，水稻根內(nèi)的NO含量下降[30]，而在同樣的100 μmol/L的Cd處理24 h以后豌豆根內(nèi)的NO卻增加[27]；另外，Chen等在研究大麥的兩種基因型weishuobuzhi和dong17對(duì)5 μmol/L的Cd脅迫反應(yīng)時(shí)發(fā)現(xiàn)，兩種基因型大麥體內(nèi)的NO含量變化不同，其中weishuobuzhi型大麥根內(nèi)的NO含量在第1天達(dá)到最大值，而后隨著處理時(shí)間的延長(zhǎng)，NO含量迅速下降，而對(duì)Cd敏感的dong17型大麥根內(nèi)的NO含量在第10天達(dá)到最大，另外，weishuobuzhi和dong17葉內(nèi)的NO含量都在第1天達(dá)到最大[31]。這些發(fā)現(xiàn)說(shuō)明重金屬脅迫下，植物體內(nèi)內(nèi)源NO含量的變化與植物的種類(lèi)以及基因型和植物組織類(lèi)型有關(guān)。

盡管大量研究證明植物在重金屬脅迫下內(nèi)源NO含量的變化受植物種類(lèi)與基因型，重金屬濃度以及處理時(shí)間等因素的影響，然而對(duì)植物懸浮細(xì)胞而言，重金屬脅迫下其內(nèi)源NO含量總會(huì)增加[32- 35]，出現(xiàn)這一現(xiàn)象的原因可能是植物懸浮細(xì)胞缺少細(xì)胞與細(xì)胞之間的網(wǎng)絡(luò)調(diào)控。

3 外源NO增強(qiáng)植物對(duì)重金屬脅迫的抗性

3.1 外源NO緩解重金屬對(duì)植物細(xì)胞造成的氧化脅迫

金屬硫蛋白(Metallothioneins, MTs)是一種小分子的富含半胱氨酸的金屬結(jié)合蛋白，通過(guò)巰基與重金屬結(jié)合形成無(wú)毒或低毒絡(luò)合物，從而避免有害重金屬對(duì)植物體的潛在毒性[59]。在動(dòng)物細(xì)胞中，有研究發(fā)現(xiàn)在肝細(xì)胞中添加NO的供體V-PYRRO/NO，能夠大幅增強(qiáng)MTs基因的表達(dá)，從而減弱Cd的毒性，增強(qiáng)肝細(xì)胞對(duì)Cd脅迫的抗性[60]。原因可能是NO能夠替換與MTs結(jié)合的重金屬[61- 62]，并且釋放的重金屬能夠進(jìn)一步促進(jìn)MTs基因的表達(dá)[62]。在植物細(xì)胞中可能也存在類(lèi)似的機(jī)制，Wang等研究發(fā)現(xiàn)外源NO能夠提高M(jìn)Ts的含量并增強(qiáng)番茄對(duì)Cu脅迫的抗性，而對(duì)MTs敏感型番茄而言，外源NO并不能明顯地增強(qiáng)番茄對(duì)Cu脅迫的抗性，表明MTs在介導(dǎo)NO緩解重金屬脅迫上起著至關(guān)重要的作用[42]。

3.2 外源NO影響植物對(duì)重金屬的吸收以及重金屬在植物體內(nèi)的分布

研究發(fā)現(xiàn)外源NO能夠影響植物對(duì)重金屬的吸收，從而調(diào)節(jié)植物對(duì)重金屬脅迫的抗性。如外源NO能夠減少苜蓿對(duì)Cd的吸收[47]，減少小麥和豌豆對(duì)Zn的過(guò)量積累[63]，抑制決明對(duì)鋁(Al)的吸收[64]。但也有研究表明外源NO緩解重金屬脅迫并不是通過(guò)抑制植物對(duì)重金屬的吸收。如外源NO緩解過(guò)量Cu對(duì)小麥種子萌發(fā)的抑制作用，并且通過(guò)增強(qiáng)SOD和CAT的活性緩解過(guò)量Cu對(duì)小麥種子造成的氧化脅迫，然而外源NO并不能抑制小麥種子對(duì)Cu的吸收[65]；外源NO能夠緩解過(guò)量Cu對(duì)人參造成的毒害作用，但并不能顯著降低人參根細(xì)胞對(duì)Cu的吸收[46]；外源NO供體硝普鈉(SNP)處理能夠緩解鉛(Pb)對(duì)擬南芥的毒害，但不能減少擬南芥對(duì)Pb的積累[66]。Xu等在研究中發(fā)現(xiàn)，對(duì)Cd的超富集植物龍葵而言，Cd脅迫能夠誘導(dǎo)顯著的生長(zhǎng)抑制，促進(jìn)H2O2的積累并破壞膜的完整性，而添加外源的NO供體亞硝基谷胱甘肽(GSNO)能夠增強(qiáng)SOD和CAT的活性，增加Pro含量，抑制H2O2的積累，提高膜的完整性，從而緩解Cd對(duì)龍葵的毒害，并且添加NO清除劑c-PTIO后能夠加重Cd對(duì)龍葵的毒害作用，這表明外源NO能夠緩解Cd脅迫對(duì)龍葵造成的傷害，增強(qiáng)龍葵對(duì)Cd脅迫的抗性。但另一方面添加NO清除劑c-PTIO能夠減少龍葵對(duì)Cd的吸收，添加外源的NO供體GSNO能夠增加龍葵對(duì)Cd的吸收，說(shuō)明NO促進(jìn)龍葵根部對(duì)Cd的吸收[67]，這些研究暗示了外源NO可能存在相應(yīng)的調(diào)節(jié)機(jī)制調(diào)節(jié)重金屬在植物體內(nèi)的分布。

面對(duì)重金屬脅迫，植物細(xì)胞也有一系列的防御機(jī)制，以最大程度減少重金屬可能造成的傷害，其中作為抗重金屬脅迫的第一道屏障，細(xì)胞壁對(duì)吸收的重金屬具有束縛作用[68]。研究表明進(jìn)入水稻細(xì)胞的Al 80%—85%分布在細(xì)胞壁，從而有效地阻止Al進(jìn)入水稻細(xì)胞的細(xì)胞質(zhì)[69]，因此重金屬在植物細(xì)胞壁的沉積是一種十分重要的重金屬抗性機(jī)制。纖維素、半纖維素和果膠等是細(xì)胞壁的主要組成成分，研究發(fā)現(xiàn)外源NO能夠調(diào)節(jié)植物細(xì)胞細(xì)胞壁成分的代謝，如低濃度的NO供體SNP能夠促進(jìn)番茄根部細(xì)胞壁纖維素的合成[70]，增加土豆嫩葉胼胝質(zhì)的積累和沉積，而高濃度的NO卻起相反的效果[71]；另外外源NO能夠減少煙草BY- 2懸浮細(xì)胞細(xì)胞壁果膠、半纖維素和纖維素的含量[72]，這些研究都暗示了外源NO可能通過(guò)調(diào)節(jié)植物細(xì)胞壁成分含量進(jìn)而調(diào)節(jié)植物細(xì)胞對(duì)重金屬脅迫的抗性。實(shí)際上，Xiong等證實(shí)了在Cd脅迫下，外源NO能夠增加水稻根細(xì)胞細(xì)胞壁果膠和半纖維素的含量，增加Cd在根和莖細(xì)胞壁中的積累，減少Cd在葉片可溶性成分中的分布，從而增強(qiáng)水稻對(duì)Cd脅迫的抗性[73]；Zhang等也發(fā)現(xiàn)外源NO能夠逆轉(zhuǎn)Al脅迫誘導(dǎo)的水稻根細(xì)胞細(xì)胞壁果膠和半纖維素含量的增加，并且顯著降低Al在水稻根尖和水稻幼苗細(xì)胞壁中的積累，從而減少Al脅迫誘導(dǎo)的根的生長(zhǎng)抑制和膜脂過(guò)氧化，增強(qiáng)水稻對(duì)Al脅迫的抗性[74]；另外外源NO能夠降低小麥和黑麥根尖細(xì)胞細(xì)胞壁對(duì)Al的吸附，增強(qiáng)Al脅迫抗性[75]。因此上述的外源NO緩解重金屬脅迫，但并不減少植物對(duì)重金屬的吸收可能是因?yàn)橥庠碞O能夠調(diào)節(jié)植物細(xì)胞細(xì)胞壁成分，促進(jìn)了重金屬在細(xì)胞壁的分布，減少了在細(xì)胞可溶性成分中的分布，但總體上并沒(méi)有減少植物體內(nèi)的重金屬，但也可能是因?yàn)榇嬖谄渌恼{(diào)節(jié)機(jī)制。

4 內(nèi)源NO參與調(diào)節(jié)植物重金屬脅迫抗性

盡管大量的研究表明低濃度的外源NO能夠緩解重金屬脅迫對(duì)植物造成的傷害，增強(qiáng)植物對(duì)重金屬脅迫的抗性，然而內(nèi)源NO在調(diào)節(jié)植物重金屬脅迫抗性上的功能角色仍存在爭(zhēng)議。有研究發(fā)現(xiàn)內(nèi)源NO是有益的，能夠緩解重金屬脅迫誘導(dǎo)的毒性，如Tian等發(fā)現(xiàn)Al脅迫能夠減少芙蓉葵體內(nèi)的內(nèi)源NO含量，并且抑制芙蓉葵根的伸長(zhǎng)，添加NO的供體SNP能夠緩解Al對(duì)根的伸長(zhǎng)抑制，而NO清除劑c-PTIO、NOS抑制劑L-NAME以及NR抑制劑鎢酸鹽(Tungstate)卻能夠加重Al的抑制作用或者抑制外源NO的緩解作用，表明內(nèi)源NO能夠促進(jìn)根的伸長(zhǎng)，增強(qiáng)Al脅迫抗性[76]；Qiu等研究發(fā)現(xiàn)微波處理能夠提高小麥抗氧化酶SOD、POD、CAT、APX、GPX和GR活性以及GSH、ASA和Car含量，緩解Cd脅迫對(duì)小麥的氧化傷害，而NO清除劑c-PTIO則能夠逆轉(zhuǎn)微波的緩解效果，表明內(nèi)源NO能夠增強(qiáng)小麥體內(nèi)的抗氧化系統(tǒng)，參與微波增強(qiáng)植物對(duì)重金屬脅迫的抗性反應(yīng)[77]；Talukdar等發(fā)現(xiàn)內(nèi)源NO與外源NO都能緩解As對(duì)菜豆造成的生長(zhǎng)抑制和氧化脅迫，增強(qiáng)菜豆As脅迫抗性[78]；在薺菜中也有類(lèi)似的發(fā)現(xiàn)，即外源NO與內(nèi)源NO都能夠緩解Cd誘導(dǎo)的膜質(zhì)過(guò)氧化，增強(qiáng)薺菜對(duì)Cd脅迫的抗性[79]。

植物螯合素(Phytochelatins, PCs)是一種由半胱氨酸、谷氨酸和甘氨酸組成的含巰基螯合多肽，GSH是植物螯合素的前體，在植物螯合素合成酶催化下合成植物螯合素，植物螯合素能夠與進(jìn)入細(xì)胞內(nèi)的重金屬離子螯合，并把重金屬隔離在液泡內(nèi)，從而減輕重金屬對(duì)細(xì)胞質(zhì)中活性物質(zhì)的毒害，增強(qiáng)植物對(duì)重金屬脅迫的抗性[59]。NO能夠促進(jìn)γ-ecs和gshs基因的表達(dá)，從而增加苜蓿根內(nèi)GSH的含量[82]，而GSH是植物螯合素的前體，因此可以推斷出NO也可能促進(jìn)植物螯合素的合成，但事實(shí)并非如此，在100 μmol/L和150 μmol/L Cd脅迫下，擬南芥懸浮細(xì)胞內(nèi)的植物螯合素含量大幅升高，而添加NOS抑制劑L-NAME后，植物螯合素含量進(jìn)一步升高，表明內(nèi)源NO抑制植物螯合素含量的升高，進(jìn)一步研究發(fā)現(xiàn)是因?yàn)镃d誘導(dǎo)的內(nèi)源NO能夠通過(guò)s-亞硝基化作用與植物螯合素的半胱氨酸殘基(Cys)結(jié)合形成NO-PC2、NO-PC3和NO-PC4，從而弱化植物螯合素對(duì)Cd的解毒作用，促進(jìn)擬南芥懸浮細(xì)胞的細(xì)胞凋亡[33]，Elviri等也證實(shí)了NO與植物螯合素的s-亞硝基化結(jié)合[83]。由此可見(jiàn)，內(nèi)源NO能夠使植物螯合素s-亞硝基化，減弱植物螯合素對(duì)重金屬脅迫的解毒功能，從而促進(jìn)重金屬對(duì)植物的毒害。

5 研究展望

盡管?chē)?guó)內(nèi)外已對(duì)NO在調(diào)節(jié)植物重金屬脅迫抗性方面做了大量研究，但是NO的具體作用機(jī)制仍然不是很清楚。建議未來(lái)相關(guān)研究應(yīng)該加強(qiáng)以下幾個(gè)方面的工作：

(1) 重視NO與其他信號(hào)分子如Ca2+、茉莉酸(JA)、水楊酸(SA)和乙烯(ET)等存在的交叉調(diào)控。研究表明其他的信號(hào)分子也參與調(diào)節(jié)植物重金屬脅迫抗[84]，并且NO可能與其他信號(hào)分子共同作用來(lái)調(diào)節(jié)植物重金屬脅迫抗性，因此加強(qiáng)NO與其他信號(hào)分子的交叉調(diào)控尤為必要。

(2) 加強(qiáng)對(duì)NO靶標(biāo)分子的研究。NO作為一種信號(hào)分子，必然通過(guò)刺激靶標(biāo)分子而發(fā)揮作用，而NO正是通過(guò)靶標(biāo)分子進(jìn)而調(diào)節(jié)植物對(duì)重金屬脅迫的抗性，因此加強(qiáng)對(duì)NO靶標(biāo)分子的研究很有意義。

(3) 加強(qiáng)對(duì)內(nèi)源NO作用機(jī)制的探討。重金屬脅迫下，內(nèi)源NO對(duì)植物重金屬脅迫抗性起著更為重要的作用，并且研究發(fā)現(xiàn)內(nèi)源NO對(duì)重金屬脅迫具有雙重作用，因此內(nèi)源NO在重金屬脅迫抗性上具體的功能角色有待研究。

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Advances on effects of nitric oxide on resistances of plants to heavy metal stress

XIA Haiwei, SHI Guoxin*, HUANG Min, WU Juan

JiangsuKeyLaboratoryofBiodiversityandBiotechnology，CollegeofLifeScience，NanjingNormalUniversity，Nanjing210023,China

Heavy metal pollution has become an increasingly serious environmental problem because heavy metal can be easily taken up by plants, leading to inhibition of plant growth and development. Hence, it is necessary to investigate resistances of plants to heavy metal stress. As an important signaling molecule, nitric oxide (NO) is involved in the regulation of multiple plant responses to a variety of abiotic and biotic stresses. Recently, an increasing number of articles have reported the effects of NO on resistances of plants to heavy metal stress. However, studies which systematically summarize the molecular mechanisms of NO on resistances of plants to heavy metal stress are quite limited. This research mainly reviews the pathways of NO production, changes of endogenous NO contents under heavy metal stress and influences of exogenous and endogenous NO on resistances to heavy metal stress. The sources of NO production in plants involve not only enzymatic reaction pathways which include nitric oxide synthase (NOS), nitrate reductase (NR), nitrite reductase (Ni-NOR) pathways and etc. but also non-enzymatic reaction pathways. Many authors have noted discrepant reports on the effects of heavy metal stress on endogenous NO content in plants and the observed differences in endogenous NO accumulation are frequently ascribed to the use of different duration of treatment, heavy metal concentrations, species and genotypes of plants and varieties of plant tissues. Interestingly, all plant cell suspensions show a visible increase in endogenous NO accumulation under heavy metal stress, which is attributed to the fact that they are lacking of network regulation between cells and cells. Meanwhile, it has been demonstrated that exogenous NO could enhance antioxidant defence system of plant cells, act as an antioxidant promoting direct scavenging of reactive oxygen species, induce metallothioneins (MTs) gene expression by displacing heavy metal bound to MTs, and affect the uptake of heavy metal into plants and the distribution of heavy metal in plant cells though regulating the metabolism of cell wall composition, consequently relieve heavy metal toxicity and enhance resistances of plants to heavy metal stress. However, the functional roles of endogenous NO in regulating resistances of plants to heavy metal stress are controversial. Some research show that endogenous NO is helpful for alleviating heavy metal-induced toxicity. On the contrary, some evidences indicate that endogenous NO is harmful, and participates in heavy metal-induced cell toxicity and programmed cell death through accelerating the absorption of heavy metal andS-nitrosylation of phytochelatins. These conflicting data suggest that NO may have a dual effect on resistances to heavy metal stress, but great efforts are required in order to clarify this speculation. Finally, interrelated perspectives are also discussed to further study the relationship between NO and resistances to heavy metal stress.

exogenous NO; endogenous NO; heavy metal stress; resistances

江蘇高校優(yōu)秀學(xué)科建設(shè)工程資助項(xiàng)目(164320H106)

2013- 06- 24;

2014- 05- 30

10.5846/stxb201306241770

*通訊作者Corresponding author.E-mail: gxshi@njnu.edu.cn

夏海威，施國(guó)新，黃敏，吳娟.一氧化氮對(duì)植物重金屬脅迫抗性的影響研究進(jìn)展.生態(tài)學(xué)報(bào),2015,35(10):3139- 3147.

Xia H W, Shi G X, Huang M, Wu J.Advances on effects of nitric oxide on resistances of plants to heavy metal stress.Acta Ecologica Sinica,2015,35(10):3139- 3147.