熊大芾(Xiong Dafu， 2nd Peop Hosp Zhuhai City，Zhuhai，Guangdong 519020)…∥Chin J Clin Basic Orthop Res.－2010，2(2).－148～152

The establishment of a stable PC-3 cell line overexpressing micro RNA-145

熊大芾(Xiong Dafu， 2nd Peop Hosp Zhuhai City，Zhuhai，Guangdong 519020)…∥Chin J Clin Basic Orthop Res.－2010，2(2).－148～152

ObjectiveTo establish stable prostate cancer bone metastasis cell line overexpressing microRAN-145(miR-145)for the study of the mechanism of miR-145 in bone metastasis.MethodspMSCV-miR-145 plasmids and retroviruses of pMSCV-vector or pMSCV-miR-145 were constructed.Both retrovirucese were transfected into bone metastasis PC-3 cell line respectively.After selection and identification，the stable cell lines named PC-3/pMSCV-vector and PC-3/pMSCV-miR-145 were obtained.ResultsThe proportion of transfected cells were approximately 80%－90%in PC-3/pMSCV-miR-145 cell line group(experimental group)and PC-3/pMSCV-vector cell line group(control group).Real-time fluorescent quantitative RT-PCR showed miR-145 expression level of experimental group was 2 000 folds higher than that of control group，and the difference had statistical significance between two groups(P ＜ 0.05).ConclusionThe establishment of stable prostate cancer bone metastasis cell line overexpressing miR-145 provides foundations to investigate the role of miR-145 in the mechanjsm of prostate cancer metastasis to bone.21 refs，3 figs，1 tab.

(Authors)