湯蘇陽(yáng)等

湯蘇陽(yáng)艾玉峰董兆麟楊青芳司徒鎮(zhèn)強(qiáng)

摘要目的:本文對(duì)新研制的聚β羥基丁酯(PHB)這一生物可降解材料進(jìn)行細(xì)胞相容性研究。方法:采用L929小鼠成纖維細(xì)胞,經(jīng)材料高溫浸提液、常溫浸出液與L929細(xì)胞接觸及直接在PHB膜片上種植該細(xì)胞,運(yùn)用四唑鹽比色法(MTT assay)測(cè)定其1,3,5天L929細(xì)胞的相對(duì)增殖率及其毒性程度。結(jié)果:PHB材料在高溫及常溫下物理、化學(xué)性質(zhì)穩(wěn)定。結(jié)論:PHB細(xì)胞相容性好,細(xì)胞毒性程度0級(jí)。

關(guān)鍵詞PHB生物降解材料細(xì)胞相容性比色法

THE EVALUATION AT THE CYTOTOXITY OFPOLY-β-HYDROXYBUTYRATE AT THE FIBROBLASTS OF MICE IN VITRO

Tang Suyang,Ai Yufeng,Dong Zhaolin,et al

Chinese PLA 205 Hospital (Jingzhou 121000)

AbstractAin:Application of MTT assay to evaluate the cytotoxity of poly-3-hydroxybutgrate at the fibroblasts of mice.Methods:Applicaioh tion logarithmic growth of L929